Canine parvovirus type 2c in Vietnam continues to produce distinct descendants with new mutations restricted to Vietnamese variants

2021 ◽  
Author(s):  
Huong Thi Thanh Doan ◽  
Xuyen Thi Kim Le ◽  
Roan Thi Do ◽  
Khue Thi Nguyen ◽  
Thanh Hoa Le
Keyword(s):  
Canine Parvovirus ◽  
New Mutations

scholarly journals Correction to: Canine parvovirus type 2c in Vietnam continues to produce distinct descendants with new mutations restricted to Vietnamese variants

2021 ◽  
Author(s):  
Huong Thi Thanh Doan ◽  
Xuyen Thi Kim Le ◽  
Roan Thi Do ◽  
Khue Thi Nguyen ◽  
Thanh Hoa Le
Keyword(s):  
Canine Parvovirus ◽  
New Mutations

scholarly journals Canine parvovirus type 2c in Vietnam continues to form distinct descendants with new mutations restricted to the Vietnamese variants

2021 ◽  
Author(s):  
THANH HOA LE ◽  
Huong Thi Thanh Doan ◽  
Xuyen Thi Kim Le ◽  
Roan Thi Do ◽  
Khue Thi Nguyen
Keyword(s):  
Amino Acids ◽  
Phylogenetic Tree ◽  
Viral Protein ◽  
Clinical Symptoms ◽  
Complete Sequence ◽  
Canine Parvovirus ◽  
The Novel ◽  
Vaccine Failure ◽  
The Common ◽  
New Mutations

Abstract The complete sequence for viral protein 2 (VP2) of canine parvovirus (CPV) was obtained from 33 isolates collected from dogs with clinical symptoms and a vaccine in Vietnam from Mar 2017– June 2019. Molecular analysis revealed the descent evolution in CPV-2c-“new”, forming a “new var.” sub-lineage of the substantial 5G, 447M mutations restricted to the Vietnamese isolates in the epitope stretches. These were found besides the common 80R, 87L, 93N, 101T, 103A, 232I, 267Y, 297A, 300G, 305Y, 323N, 324I, 334A, 341P, 370R, 426E, 440T, 447I, 555V, 564S, and 568G typical to the “new” CPV-2c. The novel 447M mutation seemed to emerge in recent years in Vietnam and was present in 38 of 58 CPV-2c strains isolated from December 2016 to June 2019 by our retrospective analysis, counting for 65.5%. With strong nodal support (98%), the topology of the phylogenetic tree revealed the substantially distinct Vietnamese sub-lineage of 2c-“new-var.” (5G/426E/447M) separated from the Vietnamese 2c-“new” (5G/426E/447I), within the 2c-(Asia)/Asia-2c. The Asia-2c strains of Vietnam were grouped with the recent Thai, Chinese, Mongolian, Taiwanese, Korean, and the Asian variants newly imported into Italy, Egypt, and Nigeria from Asia. The changes of amino acids in the epitope stretch in VP2 have effective alteration not only on the continual formation of substantial sub- or new genotypic variants but may be of subjects for antigenicity/immunogenicity and virulence to the CPV-2 strains and vaccine failure worldwide.

Peptide vaccine against canine parvovirus: identification of two neutralization subsites in the N terminus of VP2 and optimization of the amino acid sequence.

1995 ◽  
Vol 69 (11) ◽  
pp. 7274-7277 ◽  
Author(s):  
J I Casal ◽  
J P Langeveld ◽  
E Cortés ◽  
W W Schaaper ◽  
E van Dijk ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Peptide Vaccine ◽  
Canine Parvovirus ◽  
N Terminus

scholarly journals Cerebellar hypoplasia and dysplasia in a juvenile raccoon with parvoviral infection

2020 ◽  
Vol 32 (3) ◽  
pp. 463-466 ◽  
Author(s):  
Arno Wünschmann ◽  
Robert Lopez-Astacio ◽  
Anibal G. Armien ◽  
Colin R. Parrish
Keyword(s):  
Granule Cells ◽  
Nonstructural Protein ◽  
Canine Parvovirus ◽  
Cerebellar Hypoplasia ◽  
Diagnostic Laboratory ◽  
Vp2 Gene ◽  
Nonstructural Protein 1 ◽  
Cell Processes ◽  
Ns1 Gene ◽  
Pcr Targeting

A juvenile raccoon ( Procyon lotor) was submitted dead to the Minnesota Veterinary Diagnostic Laboratory for rabies testing without history. The animal had marked hypoplasia of the cerebellum. Histology demonstrated that most folia lacked granule cells and had randomly misplaced Purkinje cells. Immunohistochemistry revealed the presence of parvoviral antigen in a few neurons and cell processes. PCR targeting feline and canine parvovirus yielded a positive signal. Sequencing analyses from a fragment of the nonstructural protein 1 ( NS1) gene and a portion of the viral capsid protein 2 ( VP2) gene confirmed the presence of DNA of a recent canine parvovirus variant (CPV-2a–like virus) in the cerebellum. Our study provides evidence that (canine) parvovirus may be associated with cerebellar hypoplasia and dysplasia in raccoons, similar to the disease that occurs naturally and has been reproduced experimentally by feline parvoviral infection of pregnant cats, with subsequent intrauterine or neonatal infections of the offspring.

scholarly journals Identification of new mutations in sterol 27-hydroxylase gene in Japanese patients with cerebrotendinous xanthomatosis (CTX).

1994 ◽  
Vol 35 (6) ◽  
pp. 1031-1039
Author(s):  
K S Kim ◽  
S Kubota ◽  
M Kuriyama ◽  
J Fujiyama ◽  
I Björkhem ◽  
...  
Keyword(s):  
Japanese Patients ◽  
Cerebrotendinous Xanthomatosis ◽  
Hydroxylase Gene ◽  
New Mutations

scholarly journals Mutational Analysis of a Histone Deacetylase in Drosophila melanogaster: Missense Mutations Suppress Gene Silencing Associated With Position Effect Variegation

Genetics ◽  
2000 ◽  
Vol 154 (2) ◽  
pp. 657-668 ◽  
Author(s):  
Randy Mottus ◽  
Richard E Sobel ◽  
Thomas A Grigliatti
Keyword(s):  
Drosophila Melanogaster ◽  
Histone Deacetylase ◽  
Transcriptional Activity ◽  
Mutational Analysis ◽  
Position Effect ◽  
Transcriptional Regulator ◽  
Position Effect Variegation ◽  
Missense Mutations ◽  
Effect Variegation ◽  
New Mutations

Abstract For many years it has been noted that there is a correlation between acetylation of histones and an increase in transcriptional activity. One prediction, based on this correlation, is that hypomorphic or null mutations in histone deacetylase genes should lead to increased levels of histone acetylation and result in increased levels of transcription. It was therefore surprising when it was reported, in both yeast and fruit flies, that mutations that reduced or eliminated a histone deacetylase resulted in transcriptional silencing of genes subject to telomeric and heterochromatic position effect variegation (PEV). Here we report the first mutational analysis of a histone deacetylase in a multicellular eukaryote by examining six new mutations in HDAC1 of Drosophila melanogaster. We observed a suite of phenotypes accompanying the mutations consistent with the notion that HDAC1 acts as a global transcriptional regulator. However, in contrast to recent findings, here we report that specific missense mutations in the structural gene of HDAC1 suppress the silencing of genes subject to PEV. We propose that the missense mutations reported here are acting as antimorphic mutations that “poison” the deacetylase complex and propose a model that accounts for the various phenotypes associated with lesions in the deacetylase locus.

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