Complete genome sequences of anemone mosaic virus and ranunculus mild mosaic virus isolated from anemone imported from the Netherlands into Japan

2021 ◽  
Author(s):  
Yuya Imamura ◽  
Moritsugu Oishi ◽  
Yuji Fujiwara ◽  
Hironobu Yanagisawa
Keyword(s):  
The Netherlands ◽  
Mosaic Virus ◽  
Complete Genome ◽  
Mild Mosaic ◽  
Genome Sequences ◽  
Mild Mosaic Virus

scholarly journals First Report of the Complete Genome Sequences of Anemone Mosaic Virus and Ranunculus Mild Mosaic Virus Isolated From Anemone Imported From the Netherlands

2021 ◽  
Author(s):  
Yuya Imamura ◽  
Moritsugu Oishi ◽  
Yuji Fujiwara ◽  
Hironobu Yanagisawa
Keyword(s):  
The Netherlands ◽  
Mosaic Virus ◽  
Complete Genome ◽  
Mild Mosaic ◽  
Coding Region ◽  
Genome Sequences ◽  
Coat Protein Region ◽  
Complete Sequences ◽  
Mild Mosaic Virus ◽  
Sequence Identities

Abstract The anemone mosaic virus (AnMV) and ranunculus mild mosaic virus (RanMMV) infects the anemone plant with characteristic mosaic patterns on leaves. Two complete genome sequences of the two viruses imported from the Netherlands, were determined based on deep sequencing for the first time. Each of AnMV and RanMMV had 9,698 and 9,537 nucleotides (nt), excluding the poly(A) tail. They shared 80.0% nt/amino acid (aa) sequence identities or more, which are above the species demarcation value, with only AnMV and RanMMV reported previously in coat protein region, but having 68.0% nt/aa sequence identities or less with other potyviruses in each coding region of the complete sequences. Additionally, phylogenetic analysis showed that AnMV and RanMMV were included in other known potyviruses. These results suggest that both of AnMV and RanMMV were independent species belonging to the genus Potyvirus.

scholarly journals Complete genome sequence of a novel zantedeschia mild mosaic virus isolate: the first report from Australia and from Alocasia sp.

2016 ◽  
Vol 161 (4) ◽  
pp. 1079-1082 ◽  
Author(s):  
Dawit B. Kidanemariam ◽  
Adane D. Abraham ◽  
Amit C. Sukal ◽  
Timothy A. Holton ◽  
James L. Dale ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Isolate ◽  
Mild Mosaic ◽  
First Report ◽  
Mild Mosaic Virus

The complete genome sequence of Lamium mild mosaic virus, a member of the genus Fabavirus

2013 ◽  
Vol 158 (11) ◽  
pp. 2405-2408 ◽  
Author(s):  
Ezequiel A. Rangel ◽  
Inmaculada Ferriol ◽  
Stefano Panno ◽  
Salvatore Davino ◽  
Antonio Olmos ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

The complete genome sequence of a Brazilian isolate of yam mild mosaic virus

2012 ◽  
Vol 158 (2) ◽  
pp. 515-518 ◽  
Author(s):  
Francisco de Assis Câmara Rabelo Filho ◽  
Cícero Nicolini ◽  
Renato de Oliveira Resende ◽  
Genira Pereira de Andrade ◽  
Gilvan Pio-Ribeiro ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Mild Mosaic ◽  
Brazilian Isolate ◽  
Mild Mosaic Virus

The truth about Pea mild mosaic virus

2014 ◽  
Vol 43 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Z. Perez-Egusquiza ◽  
J. Z. Tang ◽  
L. I. Ward ◽  
J. D. Fletcher
Keyword(s):  
Mosaic Virus ◽  
Mild Mosaic ◽  
Mild Mosaic Virus

Detection of Banana mild mosaic virus and Banana virus X by polyvalent degenerate oligonucleotide RT-PCR (PDO-RT-PCR)

2007 ◽  
Vol 142 (1-2) ◽  
pp. 41-49 ◽  
Author(s):  
Pierre-Yves Teycheney ◽  
Isabelle Acina ◽  
Benham E.L. Lockhart ◽  
Thierry Candresse
Keyword(s):  
Mosaic Virus ◽  
Mild Mosaic ◽  
Rt Pcr ◽  
Degenerate Oligonucleotide ◽  
Mild Mosaic Virus

scholarly journals Mealybug (Hemiptera: Pseudococcidae) Species Associated with Cacao Mild Mosaic Virus and Evidence of Virus Acquisition

Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 994
Author(s):  
Alina S. Puig ◽  
Sarah Wurzel ◽  
Stephanie Suarez ◽  
Jean-Philippe Marelli ◽  
Jerome Niogret
Keyword(s):  
Mosaic Virus ◽  
Management Strategies ◽  
Mild Mosaic ◽  
Effective Management ◽  
Coat Protein Region ◽  
Maconellicoccus Hirsutus ◽  
Virus Acquisition ◽  
Mild Mosaic Virus ◽  
Pseudococcus Comstocki ◽  
Genus One

Theobroma cacao is affected by viruses on every continent where the crop is cultivated, with the most well-known ones belonging to the Badnavirus genus. One of these, cacao mild mosaic virus (CaMMV), is present in the Americas, and is transmitted by several species of Pseudococcidae (mealybugs). To determine which species are associated with virus-affected cacao plants in North America, and to assess their potential as vectors, mealybugs (n = 166) were collected from infected trees in Florida, and identified using COI, ITS2, and 28S markers. The species present were Pseudococcus jackbeardsleyi (38%; n = 63), Maconellicoccus hirsutus (34.3%; n = 57), Pseudococcus comstocki (15.7%; n = 26), and Ferrisia virgata (12%; n = 20). Virus acquisition was assessed by testing mealybug DNA (0.8 ng) using a nested PCR that amplified a 500 bp fragment of the movement protein–coat protein region of CaMMV. Virus sequences were obtained from 34.6 to 43.1% of the insects tested; however, acquisition did not differ among species, X2 (3, N = 166) = 0.56, p < 0.91. This study identified two new mealybug species, P. jackbeardsleyi and M. hirsutus, as potential vectors of CaMMV. This information is essential for understanding the infection cycle of CaMMV and developing effective management strategies.

scholarly journals First Report of Yam mild mosaic virus in Yam in Guangxi Province, China

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1320-1320 ◽  
Author(s):  
C. Zou ◽  
J. Meng ◽  
Z. Li ◽  
M. Wei ◽  
J. Song ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Viral Genome ◽  
Terminal Region ◽  
Mild Mosaic ◽  
Rt Pcr ◽  
Degenerate Primers ◽  
Germplasm Exchange ◽  
First Report ◽  
Pcr Products ◽  
Mild Mosaic Virus

Yams (Dioscorea spp.) are widely grown in China as vegetables and herbal medicine. However, studies on viral diseases on yams are still limited. As a pilot project of a government initiative for improving yam productivity, a small study was conducted in Guangxi, a southern province of China, on viral disease in yams. Incidence of virus-like disease for the three extensively grown D. alata cultivars, GH2, GH5, and GH6, were 12 to 40%, 12 to 29%, and 11 to 25%, respectively, as found in a field survey with a five-plot sampling method in 2010. A total of 112 leaf samples showing mosaic or mottling or leaves without symptoms were collected from the cvs. GH2, GH5, GH6, and seven additional cultivars (D. alata cvs. GY2, GY23, GY47, GY69, GY62, GY72, and D. batatas cv. Tiegun). To determine if the symptoms were caused by Yam mild mosaic virus (YMMV; genus Potyvirus, family Potyviridae), total RNA was extracted from leaves with a commercial RNA purification kit (TIANGEN, Beijing, China), and reverse-transcription (RT)-PCR was conducted with a YMMV-specific primer pair (4) that amplifies the 3′-terminal portion of the viral genome. A PCR product with the predicted size of 262 bp was obtained from samples of GH5 (number testing positive of total number of leaves = 5 of 12), GH6 (24 of 42), and GY72 (1 of 1), but not from asymptomatic leaves. PCR products from a GH5 sample (YMMV-Nanning) and a GH6 sample (YMMV-Luzhai) were cloned and sequenced using an ABI PRISM 3770 DNA Sequencer. The two PCR products were 97% identical at nucleotide (nt) level and with the highest homology (89% identity) to a YMMV isolate (GenBank Accession No. AJ305466). To further characterize the isolates, degenerate primers (2) were used to amplify viral genome sequence corresponding to the C-terminal region of the nuclear inclusion protein b (NIb) and the N-terminal region of the coat protein (CP). These 781-nt fragments were sequenced and a new primer, YMMV For1 (5′-TTCATGTCGCACAAAGCAGTTAAG-3′) corresponding to the NIb region, was designed and used together with primer YMMV UTR 1R to amplify a fragment that covers the complete CP region of YMMV by RT-PCR. These 1,278-nt fragments were sequenced (GenBank Accession Nos. JF357962 and JF357963). CP nucleotide sequences of the YMMV-Nanning and YMMV-Luzhai isolates were 94% similar, while amino acid sequences were 99% similar. BLAST searches revealed a nucleotide identity of 82 to 89% and a similarity of 88 to 97% for amino acids to sequences of YMMV isolates (AF548499 and AF548519 and AAQ12304 and BAA82070, respectively) in GenBank. YMMV is known to be prevalent on D. alata in Africa and the South Pacific, and has recently been identified in the Caribbean (1) and Colombia (3). To our knowledge, this is the first report of the natural occurrence of YMMV in China and it may have implications for yam production and germplasm exchange within China. References: (1) M. Bousalem and S. Dallot. Plant Dis. 84:200, 2000. (2) D. Colinet et al. Phytopathology 84:65, 1994. (3) S. Dallot et al. Plant Dis. 85:803, 2001. (4) R. A. Mumford and S. E. Seal. J. Virol. Methods 69:73, 1997.

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