First Report of the Complete Genome Sequences of Anemone Mosaic Virus and Ranunculus Mild Mosaic Virus Isolated From Anemone Imported From the Netherlands

Abstract The anemone mosaic virus (AnMV) and ranunculus mild mosaic virus (RanMMV) infects the anemone plant with characteristic mosaic patterns on leaves. Two complete genome sequences of the two viruses imported from the Netherlands, were determined based on deep sequencing for the first time. Each of AnMV and RanMMV had 9,698 and 9,537 nucleotides (nt), excluding the poly(A) tail. They shared 80.0% nt/amino acid (aa) sequence identities or more, which are above the species demarcation value, with only AnMV and RanMMV reported previously in coat protein region, but having 68.0% nt/aa sequence identities or less with other potyviruses in each coding region of the complete sequences. Additionally, phylogenetic analysis showed that AnMV and RanMMV were included in other known potyviruses. These results suggest that both of AnMV and RanMMV were independent species belonging to the genus Potyvirus.

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Complete genome sequences of anemone mosaic virus and ranunculus mild mosaic virus isolated from anemone imported from the Netherlands into Japan

Archives of Virology ◽

10.1007/s00705-021-05133-8 ◽

2021 ◽

Author(s):

Yuya Imamura ◽

Moritsugu Oishi ◽

Yuji Fujiwara ◽

Hironobu Yanagisawa

Keyword(s):

The Netherlands ◽

Mosaic Virus ◽

Complete Genome ◽

Mild Mosaic ◽

Genome Sequences ◽

Mild Mosaic Virus

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Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination

Archives of Virology ◽

10.1007/s00705-021-05063-5 ◽

2021 ◽

Author(s):

Roberto Ramos-Sobrinho ◽

Mayra M. M. Ferro ◽

Tatsuya Nagata ◽

Alina S. Puig ◽

Cory Von Keith ◽

...

Keyword(s):

Puerto Rico ◽

Mosaic Virus ◽

Theobroma Cacao ◽

Complete Genome ◽

Mild Mosaic ◽

Genome Sequences ◽

Mild Mosaic Virus ◽

Virus Isolates ◽

Theobroma Cacao L

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Mealybug (Hemiptera: Pseudococcidae) Species Associated with Cacao Mild Mosaic Virus and Evidence of Virus Acquisition

Insects ◽

10.3390/insects12110994 ◽

2021 ◽

Vol 12 (11) ◽

pp. 994

Author(s):

Alina S. Puig ◽

Sarah Wurzel ◽

Stephanie Suarez ◽

Jean-Philippe Marelli ◽

Jerome Niogret

Keyword(s):

Mosaic Virus ◽

Management Strategies ◽

Mild Mosaic ◽

Effective Management ◽

Coat Protein Region ◽

Maconellicoccus Hirsutus ◽

Virus Acquisition ◽

Mild Mosaic Virus ◽

Pseudococcus Comstocki ◽

Genus One

Theobroma cacao is affected by viruses on every continent where the crop is cultivated, with the most well-known ones belonging to the Badnavirus genus. One of these, cacao mild mosaic virus (CaMMV), is present in the Americas, and is transmitted by several species of Pseudococcidae (mealybugs). To determine which species are associated with virus-affected cacao plants in North America, and to assess their potential as vectors, mealybugs (n = 166) were collected from infected trees in Florida, and identified using COI, ITS2, and 28S markers. The species present were Pseudococcus jackbeardsleyi (38%; n = 63), Maconellicoccus hirsutus (34.3%; n = 57), Pseudococcus comstocki (15.7%; n = 26), and Ferrisia virgata (12%; n = 20). Virus acquisition was assessed by testing mealybug DNA (0.8 ng) using a nested PCR that amplified a 500 bp fragment of the movement protein–coat protein region of CaMMV. Virus sequences were obtained from 34.6 to 43.1% of the insects tested; however, acquisition did not differ among species, X2 (3, N = 166) = 0.56, p < 0.91. This study identified two new mealybug species, P. jackbeardsleyi and M. hirsutus, as potential vectors of CaMMV. This information is essential for understanding the infection cycle of CaMMV and developing effective management strategies.

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Complete genome sequence of a novel zantedeschia mild mosaic virus isolate: the first report from Australia and from Alocasia sp.

Archives of Virology ◽

10.1007/s00705-015-2745-z ◽

2016 ◽

Vol 161 (4) ◽

pp. 1079-1082 ◽

Cited By ~ 3

Author(s):

Dawit B. Kidanemariam ◽

Adane D. Abraham ◽

Amit C. Sukal ◽

Timothy A. Holton ◽

James L. Dale ◽

...

Keyword(s):

Mosaic Virus ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Virus Isolate ◽

Mild Mosaic ◽

First Report ◽

Mild Mosaic Virus

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The complete genome sequence of Lamium mild mosaic virus, a member of the genus Fabavirus

Archives of Virology ◽

10.1007/s00705-013-1732-5 ◽

2013 ◽

Vol 158 (11) ◽

pp. 2405-2408 ◽

Cited By ~ 5

Author(s):

Ezequiel A. Rangel ◽

Inmaculada Ferriol ◽

Stefano Panno ◽

Salvatore Davino ◽

Antonio Olmos ◽

...

Keyword(s):

Mosaic Virus ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Mild Mosaic ◽

Mild Mosaic Virus

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High genetic variability and evidence for plant-to-plant transfer of Banana mild mosaic virus

Journal of General Virology ◽

10.1099/vir.0.81197-0 ◽

2005 ◽

Vol 86 (11) ◽

pp. 3179-3187 ◽

Cited By ~ 25

Author(s):

Pierre-Yves Teycheney ◽

Nathalie Laboureau ◽

Marie-Line Iskra-Caruana ◽

Thierry Candresse

Keyword(s):

Mosaic Virus ◽

Sequence Divergence ◽

High Rate ◽

Mild Mosaic ◽

Coding Region ◽

Related Sequence ◽

Synonymous Mutations ◽

Plant Transfer ◽

Mild Mosaic Virus ◽

High Level

A total of 154 partial nucleotide sequences within the Banana mild mosaic virus (BanMMV) ORF1, which encodes the viral RNA-dependent RNA polymerase (RdRp), was obtained from 68 distinct infected banana accessions originating from various locations worldwide. The 310 nt sequences displayed a high level of variability with a mean pairwise nucleotide sequence divergence level of 20·4 %. This situation resulted essentially from a high rate of synonymous mutations. A similar analysis was performed for a limited selection of 10 banana accessions (30 sequences) on the region comprising approximately the last 310 nt of the BanMMV genome. This region corresponds to the 3′ end of ORF5, which encodes the coat protein (234 nt), and to the 3′ non-coding region. This analysis confirmed the high level of diversity observed in the RdRp dataset, characterized by a high level of synonymous mutations. Analysis of intra-host diversity indicated the existence of two distinct situations, with some plants containing only closely related sequence variants, whereas others contained widely divergent isolates. Analyses indicated that BanMMV genetic diversity is not structured by the geographical origin of the infected Musa accessions or by their genotype. This situation may be, in part, explained by the exchange of banana germplasm between different parts of the world and also by plant-to-plant transfer of virus isolates, the evidence for which is, for the first time, provided by this study.

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Trichosanthes kirilowii: A New Host of Cucurbit mild mosaic virus in China

Plant Disease ◽

10.1094/pdis-04-13-0447-pdn ◽

2013 ◽

Vol 97 (10) ◽

pp. 1388-1388 ◽

Cited By ~ 2

Author(s):

H. Y. Fan ◽

T. Tao ◽

S. W. Dong ◽

D. W. Li ◽

J. L. Yu ◽

...

Keyword(s):

Mosaic Virus ◽

Amino Acid Identity ◽

Mild Mosaic ◽

Coding Region ◽

Potential Threat ◽

New Host ◽

Yellow Color ◽

Trichosanthes Kirilowii ◽

Mild Mosaic Virus ◽

The Impact

Chinese cucumber (Trichosanthes kirilowii Maxim.) is a type of perennial liana plant of the Cucurbitaceae family that is mainly distributed in East Asia and northern Australia. It is an important medicinal plant and commonly used in Chinese herbalism, where it is considered to be one of the 50 fundamental herbs (2). During the summer and autumn of 2012, T. kirilowii plants showing symptoms of mild mosaic on the upper leaves and bright yellow color on the lower leaves were observed in the Haidian district of Beijing, China. Recently similar symptoms induced by Cucurbit mild mosaic virus (CuMMV) on squash have been reported. CuMMV is a new member of the genus Fabavirus in the Comovirinae subfamily, discovered in China in 2006 (1). Total RNA was extracted from five leaf samples of independent plants and used for reverse transcription with an oligo (dT)18 primer, followed by PCR with a pair of CuMMV virus-specific primers FaR13012F (5′-CGAGTGCGAGTTAGAAATTGGGATG-3′) and FaR15783R (5′-TCACTTTGAGGTGATAAAACAATCC-3′) to amplify a 2,772-bp fragment including RNA-dependent RNA polymerase (RdRp) coding region. The expected target fragment was obtained in all symptomatic plant samples but not from an asymptomatic plant. Nucleotide sequence comparison analysis showed that the virus isolated from T. kirilowii (GenBank Accession No. KC959843) had 95.33% nucleotide identity and 99.15% amino acid identity in the RdRp sequence with a CuMMV isolate from squash (GenBank Accession No. FJ194941) (1). In addition, symptomatic samples tested positive for CuMMV by Western blot using CuMMV small coat protein (SCP) specific polyclonal antibody (1). To our knowledge, this is the first report of T. kirilowii as natural host of CuMMV in China. The impact of CuMMV on T. kirilowii production remains to be determined; however, the extended host range for this virus suggests a potential threat of CuMMV to cucurbit crops in China. References: (1) S. W. Dong et al. Arch. Virol.157:597, 2012. (2) J. H. Hong et al. China Pharmacist 7:561, 2004.

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