Over-expression of Catharanthus roseus tryptophan decarboxylase and strictosidine synthase in rol gene integrated transgenic cell suspensions of Vinca minor

PROTOPLASMA ◽  
2014 ◽  
Vol 252 (1) ◽  
pp. 373-381 ◽  
Author(s):  
Priyanka Verma ◽  
Abhishek Sharma ◽  
Shamshad Ahmad Khan ◽  
Karuna Shanker ◽  
Ajay K. Mathur
Keyword(s):  
Catharanthus Roseus ◽  
Cell Suspensions ◽  
Tryptophan Decarboxylase ◽  
Strictosidine Synthase ◽  
Over Expression ◽  
Transgenic Cell ◽  
Vinca Minor ◽  
Rol Gene

scholarly journals Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus

Planta ◽  
1998 ◽  
Vol 205 (3) ◽  
pp. 414-419 ◽  
Author(s):  
Camilo Canel ◽  
M. Inês Lopes-Cardoso ◽  
Serap Whitmer ◽  
Leslie van der Fits ◽  
Giancarlo Pasquali ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Catharanthus Roseus ◽  
Tryptophan Decarboxylase ◽  
Alkaloid Production ◽  
Strictosidine Synthase ◽  
Over Expression

scholarly journals Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets

2018 ◽  
Vol 19 (5) ◽  
pp. 1805-1810 ◽  
Author(s):  
ZAHRA NOORMOHAMMADI ◽  
MARYAM TABAN ◽  
FARAH FARAHANI
Keyword(s):  
Gamma Radiation ◽  
Catharanthus Roseus ◽  
Hplc Analysis ◽  
Short Communication ◽  
Indole Alkaloids ◽  
Tryptophan Decarboxylase ◽  
Strictosidine Synthase ◽  
Gene Expressions ◽  
The Impact ◽  
Regenerated Plantlets

Noormohammadi Z, Taban M, Farahani F. 2018. Short Communication: The impact of Gamma radiation on Tdc and Str gene expressions in Catharanthus roseus regenerated plantlets. Biodiversitas 19: 1805-1810. Catharanthus roseus L.G. Don, is the essential medicinal plant with considerable attention. This plant is a rich source of terpenoid indole alkaloids (TIAs). The main alkaloids in C. roseus are vinblastine, vincristine, and ajmalicine. The tryptophan decarboxylase (Tdc) and Strictosidine synthase (Str) are key enzymes in TIA biosynthesis. In the present study, Tdc and Str gene expressions, as well as vinblastine production were evaluated in tissue culture regenerated plantlets in 4 groups; control, 60 Gy irradiation, 50 mg/L putrescine and 60 Gy irradiation + 50mg/L putrescine treatments. The results revealed significant increase in Tdc and Str gene expressions in 60 Gy irradiation + 50mg/L putrescine treated plantlets in comparison with control samples by using qPCR methods. HPLC analysis showed a higher amount of vinblastine in 60 Gy + 59 mg/L putrescine treated plantlets. Gamma radiation and putrescine as elicitor and polyamine, respectively, are able to improve vinblastine production in C. roseus.

scholarly journals Artemisinic Acid Serves as a Novel ORCA3 Inducer to Enhance Biosynthesis of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100
Author(s):  
Mingxuan Wang ◽  
Jiachen Zi ◽  
Jianhua Zhu ◽  
Shan Chen ◽  
Pu Wang ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
Fold Increase ◽  
Tryptophan Decarboxylase ◽  
Terpenoid Indole Alkaloids ◽  
Strictosidine Synthase ◽  
Octadecanoid Pathway ◽  
Artemisinic Acid ◽  
Meristematic Cells ◽  
Ap2 Domain

To investigate the effect of artemisinic acid (AA) on improving the production of terpenoid indole alkaloids (TIAs) of Catharanthus roseus cambial meristematic cells (CMCs), feeding AA to C. roseus CMCs caused 2.35-fold and 2.51-fold increases in the production of vindoline and catharanthine, respectively, compared with those of the untreated CMCs. qRT-PCR experiments showed that AA resulted in a 1.36-8.52 fold increase in the transcript levels of several related genes, including octadecanoid-derivative responsive Catharanthus AP2-domain protein 3 (ORCA3), tryptophan decarboxylase (TDC), strictosidine synthase (STR) and desacetoxyvindoline 4-hydroxylase (D4H). However, no effect was observed on the concentration of either jasmonic acid (JA), or the octadecanoid-pathway inhibitors block TIA accumulation caused by AA. The results indicated that AA might serve as a novel ORCA3 inducer to manipulate biosynthesis of TIAs in C. roseus CMCs via an unknown mechanism.

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