The first identification and characterization of a histidine-specific amino acid racemase, histidine racemase from a lactic acid bacterium, Leuconostoc mesenteroides subsp. sake NBRC 102480

Amino Acids ◽  
2018 ◽  
Vol 51 (2) ◽  
pp. 331-343 ◽  
Author(s):  
Motoyasu Adachi ◽  
Rumi Shimizu ◽  
Shiro Kato ◽  
Tadao Oikawa
Keyword(s):  
Lactic Acid ◽  
Amino Acid ◽  
Lactic Acid Bacterium ◽  
Leuconostoc Mesenteroides ◽  
Specific Amino Acid ◽  
Amino Acid Racemase ◽  
Identification And Characterization

Characterization of a novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis

2021 ◽  
Author(s):  
Ryushi Kawakami ◽  
Chinatsu Kinoshita ◽  
Tomoki Kawase ◽  
Mikio Sato ◽  
Junji Hayashi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Substrate Specificity ◽  
Enzyme Stability ◽  
Hyperthermophilic Archaea ◽  
Thermococcus Litoralis ◽  
Hyperthermophilic Archaeon ◽  
Aminobutyrate Aminotransferase ◽  
Chaperone Protein ◽  
Amino Acid Racemase

Abstract The amino acid sequence of the OCC_10945 gene product from the hyperthermophilic archaeon Thermococcus litoralis DSM5473, originally annotated as γ-aminobutyrate aminotransferase, is highly similar to that of the uncharacterized pyridoxal 5ʹ-phosphate (PLP)-dependent amino acid racemase from Pyrococcus horikoshii. The OCC_10945 enzyme was successfully overexpressed in Escherichia coli by co-expression with a chaperone protein. The purified enzyme demonstrated PLP-dependent amino acid racemase activity primarily toward Met and Leu. Although PLP contributed to enzyme stability, it only loosely bound to this enzyme. Enzyme activity was strongly inhibited by several metal ions, including Co2+ and Zn2+, and non-substrate amino acids such as l-Arg and l-Lys. These results suggest that the underlying PLP-binding and substrate recognition mechanisms in this enzyme are significantly different from those of the other archaeal and bacterial amino acid racemases. This is the first description of a novel PLP-dependent amino acid racemase with moderate substrate specificity in hyperthermophilic archaea.

scholarly journals Identification and characterization of lactic acid bacteria isolated from traditional pickles in Sichuan, China

2012 ◽  
Vol 58 (3) ◽  
pp. 163-172 ◽  
Author(s):  
Jie Yu ◽  
Wa Gao ◽  
Manjun Qing ◽  
Zhihong Sun ◽  
Weihong Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Identification And Characterization

scholarly journals Characterization of γ-Aminobutyric acid(GABA) produced by a lactic acid bacterium from button mushroom bed

2013 ◽  
Vol 11 (4) ◽  
pp. 181-186 ◽  
Author(s):  
Yun-Seok Lee ◽  
Tae-Young Song ◽  
Won-Sik Kong ◽  
Min-Ho Yoon
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Aminobutyric Acid ◽  
Button Mushroom

Characterization of a new virulent phage infecting the lactic acid bacterium Oenococcus oeni

2016 ◽  
Vol 54 ◽  
pp. 167-177 ◽  
Author(s):  
Fety Jaomanjaka ◽  
Olivier Claisse ◽  
Mélanie Blanche-Barbat ◽  
Melina Petrel ◽  
Patricia Ballestra ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Oenococcus Oeni ◽  
Virulent Phage

scholarly journals Characterization of antifungal metabolites produced by novel lactic acid bacterium and their potential application as food biopreservatives

2019 ◽  
Vol 64 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Mohamed G. Shehata ◽  
Ahmed N. Badr ◽  
Sobhy A. El Sohaimy ◽  
Dalal Asker ◽  
Tarek S. Awad
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Potential Application ◽  
Antifungal Metabolites

scholarly journals Identification and characterization of lactic acid bacteria isolated from traditional cone yoghurt

2017 ◽  
Vol 26 (6) ◽  
pp. 1625-1632 ◽  
Author(s):  
Kamil Bostan ◽  
Ayla Unver Alcay ◽  
Semiha Yalçin ◽  
Ufuk Eren Vapur ◽  
Mustafa Nizamlioglu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Identification And Characterization

scholarly journals Identification and Characterization of a New Metallo-β-Lactamase, IND-5, from a Clinical Isolate of Chryseobacterium indologenes

2007 ◽  
Vol 51 (8) ◽  
pp. 2988-2990 ◽  
Author(s):  
Mariagrazia Perilli ◽  
Bibiana Caporale ◽  
Giuseppe Celenza ◽  
Cristina Pellegrini ◽  
Jean Denis Docquier ◽  
...  
Keyword(s):  
Amino Acid ◽  
Clinical Isolate ◽  
Amino Acid Homology ◽  
Chryseobacterium Indologenes ◽  
Identification And Characterization

ABSTRACT A new natural IND-type metallo-β-lactamase variant, IND-5, was identified in a clinical isolate of Chryseobacterium indologenes. IND-5 shared 92.8% and 92.4% amino acid homology with IND-1 and IND-3, respectively. Purified enzyme (pI = 8.8, M r = 25,000) was able to hydrolyze penicillins, some narrow- and expanded-spectrum cephalosporins, and carbapenems but not monobactams.

The complete genome sequence of a lactic acid bacterium Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484T

2016 ◽  
Vol 219 ◽  
pp. 3-4
Author(s):  
Gun-Seok Park ◽  
Sung-Jun Hong ◽  
Byung Kwon Jung ◽  
Changhee Lee ◽  
Choi Kyu Park ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Leuconostoc Mesenteroides
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