Standard addition method with cumulative spikes: uric acid determination in human serum by voltammetry with optimized uncertainty

2018 ◽  
Vol 23 (6) ◽  
pp. 337-348 ◽  
Author(s):  
Tony R. L. Dadamos ◽  
Airton J. Damaceno ◽  
Fernando L. Fertonani ◽  
Ricardo J. N. B. Silva
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Addition Method ◽  
Uric Acid Determination ◽  
Acid Determination

scholarly journals THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO

2016 ◽  
Vol 11 (2) ◽  
pp. 192
Author(s):  
Abdi Naufal Ramadhan ◽  
Luluil Maknun ◽  
Noerma Juli Azhari
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Acid Concentration ◽  
Enzyme Concentration ◽  
Membrane Thickness ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Uric Acid Determination ◽  
Conductometric Biosensor ◽  
Acid Determination

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.

scholarly journals Absolute Quantification of Uric Acid in Human Urine Using Surface Enhanced Raman Scattering with the Standard Addition Method

2017 ◽  
Vol 89 (4) ◽  
pp. 2472-2477 ◽  
Author(s):  
Chloe Westley ◽  
Yun Xu ◽  
Baskaran Thilaganathan ◽  
Andrew J. Carnell ◽  
Nicholas J. Turner ◽  
...  
Keyword(s):  
Uric Acid ◽  
Raman Scattering ◽  
Human Urine ◽  
Standard Addition ◽  
Absolute Quantification ◽  
Standard Addition Method ◽  
Addition Method ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

2004 ◽  
Vol 17 (8) ◽  
pp. 701-705 ◽  
Author(s):  
R. F. Dutra ◽  
K. A. Moreira ◽  
M. I. P. Oliveira ◽  
A. N. Araújo ◽  
M. C. B. S. Montenegro ◽  
...  
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Uric Acid Determination ◽  
Acid Determination

scholarly journals THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO

2016 ◽  
Vol 11 (2) ◽  
pp. 192
Author(s):  
Abdi Naufal Ramadhan ◽  
Luluil Maknun ◽  
Noerma Juli Azhari
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Acid Concentration ◽  
Enzyme Concentration ◽  
Membrane Thickness ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Uric Acid Determination ◽  
Conductometric Biosensor ◽  
Acid Determination

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.

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