Arginine–malate-based dual-emission carbon dots for uric acid determination in human serum with a miniaturized device

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.

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An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

Electroanalysis ◽

10.1002/elan.200403142 ◽

2004 ◽

Vol 17 (8) ◽

pp. 701-705 ◽

Cited By ~ 12

Author(s):

R. F. Dutra ◽

K. A. Moreira ◽

M. I. P. Oliveira ◽

A. N. Araújo ◽

M. C. B. S. Montenegro ◽

...

Keyword(s):

Uric Acid ◽

Human Serum ◽

Flow Injection ◽

Flow Injection Analysis ◽

Uric Acid Determination ◽

Acid Determination

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THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v11i2.724 ◽

2016 ◽

Vol 11 (2) ◽

pp. 192

Author(s):

Abdi Naufal Ramadhan ◽

Luluil Maknun ◽

Noerma Juli Azhari

Keyword(s):

Uric Acid ◽

Human Serum ◽

Acid Concentration ◽

Enzyme Concentration ◽

Membrane Thickness ◽

Uric Acid Concentration ◽

Serum Samples ◽

Uric Acid Determination ◽

Conductometric Biosensor ◽

Acid Determination

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.

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Fabrication of an electrochemical biosensor based on Fe3O4 nanoparticles and uricase modified carbon paste electrode for uric acid determination

Monatshefte für Chemie - Chemical Monthly ◽

10.1007/s00706-021-02749-z ◽

2021 ◽

Author(s):

Zehra Ozden Erdogan ◽

Semahat Kucukkolbası

Keyword(s):

Uric Acid ◽

Carbon Paste Electrode ◽

Fe3o4 Nanoparticles ◽

Electrochemical Biosensor ◽

Modified Carbon Paste Electrode ◽

Carbon Paste ◽

Uric Acid Determination ◽

Paste Electrode ◽

Acid Determination

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Improved automated kinetic determination of uric acid in serum by use of uricase/catalase/aldehyde dehydrogenase.

Clinical Chemistry ◽

10.1093/clinchem/25.4.619 ◽

1979 ◽

Vol 25 (4) ◽

pp. 619-621 ◽

Cited By ~ 9

Author(s):

K Bartl ◽

M Brandhuber ◽

J Ziegenhorn

Keyword(s):

Uric Acid ◽

Aldehyde Dehydrogenase ◽

Alcohol Dehydrogenase Isoenzymes ◽

Kinetic Determination ◽

Enzymatic Determination ◽

Other Substances ◽

Inhibitor System ◽

Uric Acid Determination ◽

Acid Determination

Abstract The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydrogenase isoenzymes. Among other substances, a mixture of pyrazole and oxalate can be used to eliminate these interferences. This inhibitor system gives good results when used in the automated kinetic uric acid determination, as is shown by a comparison with the manual assay for uric acid according to Kageyama [Clin. Chim. Acta 31, 421 (1971)].

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