scholarly journals THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO

2016 ◽  
Vol 11 (2) ◽  
pp. 192
Author(s):  
Abdi Naufal Ramadhan ◽  
Luluil Maknun ◽  
Noerma Juli Azhari
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Acid Concentration ◽  
Enzyme Concentration ◽  
Membrane Thickness ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Uric Acid Determination ◽  
Conductometric Biosensor ◽  
Acid Determination

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.

scholarly journals THE DEVELOPMENT OF CONDUCTOMETRIC BIOSENSOR FOR DETERMINATION OF URIC ACID CONCENTRATION IN HUMAN SERUM USING SCREEN PRINTED CARBON ELECTRODE (SPCE) – NATA DE COCO

2016 ◽  
Vol 11 (2) ◽  
pp. 192
Author(s):  
Abdi Naufal Ramadhan ◽  
Luluil Maknun ◽  
Noerma Juli Azhari
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Acid Concentration ◽  
Enzyme Concentration ◽  
Membrane Thickness ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Uric Acid Determination ◽  
Conductometric Biosensor ◽  
Acid Determination

The control of uric acid is important. A high level of uric acid can cause gout disease. Therefore a simple, fast, and accurate method for uric acid determination is required. In this research a conductometric biosensor has been developed by SPCE – Nata de coco for uric acid determination. The prepared biosensor was optimized to get a good performance of biosensor and it is applicable for human serum samples. The optimized variables were enzyme concentration, membrane thickness and pH solution. The various enzyme concentration were 6 μg/mL; 12 μg/mL; 18μg/mL; 24 μg/mL. The various membrane thickness were 5 μm; 10 μm; 15 μm. Meanwhile, the various pH solution were 7; 7.5; 8; 8.5; 9. The optimum enzime concentration was 18 µg/mL with the membrane thickness and pH were 5 µm and 8, respectively. The prepared biosensor can determine the uric acid concentration at range of 0 – 1.2 ppm with the sensitivity of 7.74 µS/ppm and the limit detection is 0 ppm. The biosensor was applied to uric acid detection in human serum with accuracy of 95 %.

scholarly journals Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

2011 ◽  
Vol 6 ◽  
pp. ACI.S7346 ◽  
Author(s):  
Ani Mulyasuryani ◽  
Arie Srihardiastutie
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Candida Utilis ◽  
Medical Laboratory ◽  
Membrane Thickness ◽  
Serum Samples ◽  
Ph Change ◽  
Pt Electrode ◽  
Enzyme Biosensor

A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

Effect of urine storage on urinary uric acid concentrations

2000 ◽  
Vol 37 (3) ◽  
pp. 355-359 ◽  
Author(s):  
Jun-Ichi Yamakita ◽  
Tetsuya Yamamoto ◽  
Yuji Moriwaki ◽  
Sumio Takahashi ◽  
Zenta Tsutsumi ◽  
...  
Keyword(s):  
Uric Acid ◽  
Acid Concentration ◽  
Storage Temperature ◽  
Accurate Determination ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Diagnosis And Classification ◽  
Urine Storage

Accurate determination of serum and urinary uric acid concentrations is essential for the diagnosis and classification of gout according to uric acid metabolism derangement. Urine and/or serum samples are often kept at either 4°C or 20°C until assayed, when a large number of samples are handled simultaneously. Our preliminary study indicated a significant decrease in urinary uric acid concentration after preservation, regardless of the storage temperature. Uric acid crystals were often observed in these cases which showed a marked decrease in urinary uric acid concentration after storage. In the present study, we sought the factor(s) that might cause this decrease in urinary uric acid concentration, as well as measures to overcome the problem. High urinary uric acid concentration and low pH proved to play major roles in the decrease in urinary uric acid concentration after storage. In contrast, dilution of the urine samples before storage resulted in no significant change in urinary uric acid concentration. Based on these results, we recommend diluting urine before storage for determination of uric acid concentration and avoiding underestimation.

Standard addition method with cumulative spikes: uric acid determination in human serum by voltammetry with optimized uncertainty

2018 ◽  
Vol 23 (6) ◽  
pp. 337-348 ◽  
Author(s):  
Tony R. L. Dadamos ◽  
Airton J. Damaceno ◽  
Fernando L. Fertonani ◽  
Ricardo J. N. B. Silva
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Standard Addition ◽  
Standard Addition Method ◽  
Addition Method ◽  
Uric Acid Determination ◽  
Acid Determination

A CoOOH nanoflake-based light scattering probe for the simple and selective detection of uric acid in human serum

2018 ◽  
Vol 10 (40) ◽  
pp. 4951-4957 ◽  
Author(s):  
Zhi Yuan Mao ◽  
Lu Ning Zhu ◽  
Jie Gao ◽  
Jia Jun Liu ◽  
Yin Hui Wei ◽  
...  
Keyword(s):  
Uric Acid ◽  
Light Scattering ◽  
Human Serum ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Simple Strategy ◽  
Lower Light ◽  
Coooh Nanoflakes ◽  
Reduced Light ◽  
The Relationship

In the presence of uricase, uric acid generated H2O2, which further decomposed CoOOH nanoflakes to release Co2+, resulting in smaller nanoparticles with lower light scattering. Based on the relationship between the reduced light scattering and uric acid concentration, the simple strategy was applicable to uric acid sensing in human serum samples.

An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow-Injection Analysis

2004 ◽  
Vol 17 (8) ◽  
pp. 701-705 ◽  
Author(s):  
R. F. Dutra ◽  
K. A. Moreira ◽  
M. I. P. Oliveira ◽  
A. N. Araújo ◽  
M. C. B. S. Montenegro ◽  
...  
Keyword(s):  
Uric Acid ◽  
Human Serum ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Uric Acid Determination ◽  
Acid Determination
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