Comparative evaluation of the novel IMMUNOCATCHTM Streptococcus pneumoniae (EIKEN CHEMICAL CO., LTD) test with the Uni-GoldTM Streptococcus pneumoniae assay and the BinaxNOW® Streptococcus pneumoniae antigen card for the detection of pneumococcal capsular antigen in urine samples

Author(s):  
F. Congestrì ◽  
M. Morotti ◽  
R. Vicari ◽  
M. F. Pedna ◽  
M. Sparacino ◽  
...  
2016 ◽  
Vol 54 (4) ◽  
pp. 1164-1166 ◽  
Author(s):  
Francesco Congestrì ◽  
Elisabetta Crepaldi ◽  
Marina Gagliardi ◽  
Maria Federica Pedna ◽  
Vittorio Sambri

A new immunochromatographic test (bioNexiaLegionella; bioMérieux) for the detection ofLegionella pneumophilaurinary antigen was evaluated in 255 urine samples. The results were compared with those obtained by the BinaxNOW and SofiaLegionellatests. The novel test compared well with those currently in use.


2015 ◽  
Vol 5 (2) ◽  
pp. 93-97
Author(s):  
Janaki Subramanian Iyer ◽  
Leela Poonja

ABSTRACT Objective To clinically evaluate various tobacco-associated lesions and to evaluate and compare the micronucleus (MN) assay in exfoliated urothelial cells in patients with smoking and smokeless tobacco-associated lesions. Materials and Methods This study was conducted in the Department of Oral Pathology and Microbiology, Mahatma Gandhi Mission's Dental College and Hospital, Navi Mumbai, from October 2012 to June 2013. One hundred cases having tobacco habits (smoking or smokeless) and clinically detectable tobacco-associated lesions were included. Exfoliated urothelial cytosmears were prepared, stained with Papanicolaou and slides were scored for MN. Results All cases (n = 100) were found to have tobaccoassociated lesions that were clinically detectable. Voided urine samples were collected from all cases (n = 100) who indulged in smoking and smokeless tobacco habit, with males (n = 71) and females (n = 29), whose ages ranged from 19 to 75 years. We observed that out of the 100 cases evaluated, 12 cases showed the presence of MN in the urine cytosmear. Of these 12 cases, n = 10 were bidi smokers and n = 2 were betel quid chewers. Owing to insufficient population of urothelial cells in the cytosmear, MN evaluation could not be statistically proved. Conclusion Although, MN score in the urothelial cells could not be statistically assessed, due to insufficient number of urothelial cells, our observations reveal that MN count seems to be increased in smokers than smokeless tobacco users. Thus, we urge the need for further studies to highlight the comparative evaluation of MN score between smokers and smokeless tobacco on urothelial cells. How to cite this article Iyer JS, Pathak J, Patel S, Poonja L, Swain N. Comparative Evaluation of Micronuclei in Exfoliated Urothelial Cells in Patients with Smoking and Smokeless Tobacco-associated Lesions: A Prospective Quantitative Study. J Contemp Dent 2015;5(2):93-97.


2011 ◽  
Vol 66 (4) ◽  
pp. 845-849 ◽  
Author(s):  
L. Drago ◽  
L. Nicola ◽  
V. Rodighiero ◽  
M. Larosa ◽  
R. Mattina ◽  
...  

2001 ◽  
Vol 45 (3) ◽  
pp. 789-793 ◽  
Author(s):  
Jari Jalava ◽  
Janne Kataja ◽  
Helena Seppälä ◽  
Pentti Huovinen

ABSTRACT The in vitro susceptibilities of 184 erythromycin-resistant streptococci to a novel ketolide, telithromycin (HMR 3647), were tested. These clinical isolates included 111 Streptococcus pyogenes, 18 group C streptococcus, 18 group G streptococcus, and 37 Streptococcus pneumoniae strains. The MICs for all but eight S. pyogenes strains were ≤0.5 μg/ml, indicating that telithromycin is active in vitro against erythromycin-resistant Streptococcus strains. All strains for which MICs were ≥1 μg/ml had an erm(B) resistance gene and six strains for which MICs were ≥4 μg/ml had a constitutiveerm(B) gene (MIC range, 4 to 64 μg/ml). Interestingly, for S. pneumoniae strains with a constitutiveerm(B) gene, MICs were ≤0.25 μg/ml (MIC range, ≤0.008 to 0.25 μg/ml). Our in vitro data show that for S. pyogenes strains which constitutively express theerm(B) methylase gene, MICs are so high that the strains might be clinically resistant to telithromycin.


2015 ◽  
Vol 54 (2) ◽  
pp. 328-332 ◽  
Author(s):  
Jesper Iversen ◽  
Gitta Stendal ◽  
Cecilie M. Gerdes ◽  
Christian H. Meyer ◽  
Christian Østergaard Andersen ◽  
...  

This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA instrument plating 10 μl of specimen, and on two WASP instruments, one plating 1 μl of specimen (WASP-1), and the second plating 10 μl of WASP (WASP-10). All samples were incubated, analyzed, and digitally imaged using the BD Kiestra total lab automation system. The results were evaluated using a quantitative protocol and assessed for the presence or absence of ≥5 distinct colonies. Separate studies were conducted using quality control (QC) organisms to determine the relative accuracy of WASP-1, WASP-10, and InoqulA instruments compared to the results obtained with a calibrated pipette. The results with QC organisms were calculated as the ratios of the counts of the automated instruments divided by the counts for the calibrated pipette (the gold standard method). The ratios for the InoqulA instrument were closest to 1.0, with the smallest standard deviations indicating that compared to a calibrated pipette, the InoqulA results were more accurate than those with the WASP instrument. For clinical samples, the WASP instruments produced higher colony counts and more commensals than the InoqulA instrument, with differences most notable for WASP-1. The InoqulA instrument was significantly better at dispersing organisms with counts of ≥105bacteria/ml of urine than were the WASP-1 and WASP-10 instruments (P< 0.05). Our results suggest that the InoqulA quantitative results are more accurate than the WASP results, and, moreover, the number of isolated colonies produced by the InoqulA instrument was significantly greater than that produced by the WASP instrument.


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