Influence of different irrigation regimens on the dentinal tubule penetration of a bioceramic-based root canal sealer: a confocal analysis study

Abstract This study aimed to evaluate penetrability on dentinal tubule of a new bioceramic sealer through confocal laser scanning microscopy (CLSM). A specific fluorophore (Fluo-3) was mixed with the sealer. Forty distobuccal roots from maxillary molars were selected, and root canal preparation was carried out with Wave One Gold # 35.06 instruments. Roots were randomly assigned to 4 groups according to the filling procedures: Bioceramic/Lentulo (Sealer Plus BC); Bioceramic/EasyClean group, three activation of the sealer (3x20 s) with Easy Clean instrument; Bioceramic/Irrisonic: ultrasonic activation for 30 s; and AHplus/Lentulo: epoxy resin based sealer (AH Plus) was utilized with the same protocol as the BC/LE group. After 72 h, specimens were transversally sectioned at 2 and 7 mm from root apex and then analyzed through CLSM. Sealer penetration area on dentinal tubule was measured by Adobe Photoshop CC2018. Kruskal Wallis and Wilcoxon T tests were carried out. Penetrability results were similar for both sealers regardless of which technique was performed to activate them inside the root canal (p>0.05). It is reasonable to conclude that penetration of bioceramic and epoxy resin based sealers occurred unimpressively. The type of instrument used to activate bioceramic sealer did not affect penetrability. Fluo 3 should be recommended as the fluorophore to evaluate dentinal tubule penetration of bioceramic sealers.

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Effect of Calcium Hydroxide Dressing on the Dentinal Tubule Penetration of 2 Different Root Canal Sealers: A Confocal Laser Scanning Microscopic Study

Journal of Endodontics ◽

10.1016/j.joen.2018.02.016 ◽

2018 ◽

Vol 44 (6) ◽

pp. 1018-1023 ◽

Cited By ~ 13

Author(s):

Emel Uzunoglu-Özyürek ◽

Özge Erdoğan ◽

Sevinç Aktemur Türker

Keyword(s):

Calcium Hydroxide ◽

Microscopic Study ◽

Root Canal ◽

Laser Scanning ◽

Confocal Laser Scanning ◽

Confocal Laser ◽

Dentinal Tubule ◽

Tubule Penetration ◽

Root Canal Sealers

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Push-out bond strength and dentinal tubule penetration of different root canal sealers used with coated core materials

Restorative Dentistry & Endodontics ◽

10.5395/rde.2016.41.2.114 ◽

2016 ◽

Vol 41 (2) ◽

pp. 114 ◽

Cited By ~ 9

Author(s):

Derya Deniz Sungur ◽

Nuhan Purali ◽

Erdal Coşgun ◽

Semra Calt

Keyword(s):

Bond Strength ◽

Root Canal ◽

Dentinal Tubule ◽

Tubule Penetration ◽

Root Canal Sealers ◽

Push Out ◽

Core Materials

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Evaluation of dentinal tubule penetration depth and push-out bond strength of AH 26, BioRoot RCS, and MTA Plus root canal sealers in presence or absence of smear layer

Journal of Dental Research Dental Clinics Dental Prospects ◽

10.15171/joddd.2018.046 ◽

2018 ◽

Vol 12 (4) ◽

pp. 294-298 ◽

Cited By ~ 3

Author(s):

Sevinç Aktemur Türker ◽

Emel Uzunoğlu ◽

Nuhan Purali

Keyword(s):

Bond Strength ◽

Penetration Depth ◽

Root Canal ◽

Smear Layer ◽

Dentinal Tubule ◽

Limited Effect ◽

Gutta Percha ◽

Tubule Penetration ◽

Root Canal Sealers ◽

Push Out

Background.This study compared the effect of smear layer on the penetration depth and push-out bond strength of various root canal sealers. Methods.A total of 90 extracted human mandibular premolars were assigned into 2 groups: smear layer preserved and smear layer removed. Then the roots were further divided into 3 subgroups according to the sealer tested: AH 26, BioRoot RCS and MTA Plus. Obturation was performed with gutta-percha and the relevant sealer was mixed with 0.1% rhodamine B. Three 1-mm-thick slices were obtained from the mid-third area of each root. Two slices were selected for the push-out test and the remaining slice was used to calculate the dentinal tubule penetration depth and percentage. Results.The retention of MTA Plus and BioRoot RCS was higher than that of AH 26 when the smear layer was preserved (P<0.05). BioRoot RCS showed the lowest penetration depth when the smear layer was removed (P<0.05). Conclusion.Dentinal tubule penetration of root canal sealers had a limited effect on their adhesion to root canal wall.

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