Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi

2003 ◽  
Vol 9 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Muhammad Nasrum Massi ◽  
Akinobu Gotoh ◽  
Acharya Bishnu ◽  
Masato Kawabata ◽  
Toshiro Shirakawa ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Salmonella Typhi ◽  
Rapid Diagnosis ◽  
Pcr Assay ◽  
Flagellin Gene

scholarly journals A Rapid and Sensitive Diagnosis of Typhoid Fever Based On Nested PCR-Voltammetric DNA Biosensor Using Flagellin Gene Fragment

2018 ◽  
Vol 16 (1) ◽  
pp. 87 ◽  
Author(s):  
Yeni Wahyuni Hartati ◽  
Santhy Wyantuti ◽  
M. Lutfi Firdaus ◽  
Nurul Auliany ◽  
Rini Surbakti ◽  
...  
Keyword(s):  
Typhoid Fever ◽  
Dna Sequences ◽  
Nested Pcr ◽  
Differential Pulse ◽  
Salmonella Typhi ◽  
Dna Biosensor ◽  
Pencil Graphite Electrode ◽  
Flagellin Gene ◽  
Complementary Sequence ◽  
Pcr Product

Typhoid fever caused by Salmonella typhi is an important issue for public health in the world. Laboratory methods for rapid and sensitive diagnosis are very important for disease management. The purpose of this study was to determine the performance of nested PCR–voltammetric DNA biosensor using flagellin gene (fla) of S. typhi as a marker. The differential pulse voltammetry using pencil graphite electrode was applied to measure the guanine oxidation signal of probes vs synthetic target stDNA and probes vs fla PCR product hybridizations. The probe DNA selectivity was examined by hybridized probes vs non-complementary sequence. The result showed that the first round nested PCR product can not be visualized by agarose electrophoresis, whereas using the voltammetric biosensor methods can be detected both for the first or second round nested PCR product. The average peak current of hybridized probe vs first and second round of PCR product was 2.32 and 1.47 μA respectively, at 0.9 V. Detection of the DNA sequences of the infectious diseases from PCR amplified real sample was also carried out using this voltammetric DNA biosensor methods.

scholarly journals Stool shedding of Salmonella Typhi in children with blood culture-confirmed typhoid fever

2020 ◽  
Vol 101 ◽  
pp. 123
Author(s):  
M. Srinivasan ◽  
S. Giri ◽  
S. Kulandaipalayam Natarajan ◽  
N. Kumar ◽  
V.R. Mohan ◽  
...  
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Salmonella Typhi

scholarly journals Typh fevers: a combination of Typhoid and Typhus

2017 ◽  
Vol 8 (6) ◽  
pp. 40-41
Author(s):  
Robin George Manappallil
Keyword(s):  
Typhoid Fever ◽  
Similar Pattern ◽  
Salmonella Typhi ◽  
Young Lady ◽  
Medical Sciences ◽  
Rickettsial Infection ◽  
History Of ◽  
Typhus Fever

Typhoid fever is caused by Salmonella typhi, while typhus fever is a rickettsial infection. In both cases the patients present with almost similar pattern of illness and are often misdiagnosed. This is the case of a young lady who presented with 10 days history of fever and was diagnosed to have typhoid fever. She continued to be febrile despite ceftriaxone therapy. She was later found to have a typhus coinfection and improved with addition of doxycycline.Asian Journal of Medical Sciences Vol.8(6) 2017 40-41

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