Erratum to: Assessment of kidney function in children by enzymatic determination of 2-h or 24-h creatinine clearance: comparison with inulin clearance

SummaryOne hundred 125I-sodium iothalamate and endogenous creatinine clearances in 36 subjects were compared with standard inulin clearance measurements. There was a very close correlation of the urine/plasma ratios between any two of the three clearances studied, hence both labelled iothalamate and creatinine can be relied upon for the estimation of the glomerular filtration rate in clinical practice. The mean ratio of 125I-sodium iothalamate to inulin clearance was 1.01 and that of creatinine to inulin 1.03. It is concluded that the clearance of 125I-sodium iothalamate can be substituted for the clearance of inulin in clinical determinations of the glomerular filtration rate but that in centres without radioisotope facilities the endogenous creatinine clearance can be used instead.

Download Full-text

Enzymatic Determination of Hydroxysteroids in Human Skin Surface Lipids1

Journal of Investigative Dermatology ◽

10.1038/jid.1963.51 ◽

1963 ◽

Vol 41 (5) ◽

pp. 265-268 ◽

Cited By ~ 1

Author(s):

Thomas J Cook ◽

Allan L Lorincz ◽

Alan R Spector

Keyword(s):

Human Skin ◽

Skin Surface ◽

Enzymatic Determination

Download Full-text

Separate Enzymatic Determination of Hypoxanthine and Xanthine in Human Urine

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.3109/00365516509075359 ◽

1965 ◽

Vol 17 (5) ◽

pp. 454-459 ◽

Cited By ~ 16

Author(s):

Birte Brandt Petersen ◽

J. Jørni ◽

S. Jørgensen

Keyword(s):

Human Urine ◽

Enzymatic Determination

Download Full-text

Animal and vegetable fats and oils. Enzymatic determination of total sterols content

10.3403/30333398 ◽

2016 ◽

Keyword(s):

Fats And Oils ◽

Enzymatic Determination

Download Full-text

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

Clinical Chemistry ◽

10.1093/clinchem/29.8.1513 ◽

1983 ◽

Vol 29 (8) ◽

pp. 1513-1517 ◽

Cited By ~ 10

Author(s):

M W McGowan ◽

J D Artiss ◽

B Zak

Keyword(s):

Aqueous Solution ◽

Hydrogen Peroxide ◽

Amniotic Fluid ◽

Enzymatic Reaction ◽

Detergent Solution ◽

Enzymatic Determination ◽

Red Color ◽

Hydrolysis Of ◽

Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

Download Full-text

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

Analytical Methods ◽

10.1039/c9ay00718k ◽

2019 ◽

Vol 11 (30) ◽

pp. 3866-3873 ◽

Cited By ~ 1

Author(s):

R. Karthikeyan ◽

D. James Nelson ◽

S. Abraham John

Keyword(s):

Glassy Carbon ◽

Low Cost ◽

Phosphate Buffer Solution ◽

Purine Nucleotides ◽

Buffer Solution ◽

Solution Ph ◽

Carbon Dot ◽

Enzymatic Determination ◽

Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

Download Full-text