A simple shoot multiplication procedure using internode explants, and its application for particle bombardment and Agrobacterium-mediated transformation in watercress

2009 ◽  
Vol 122 (4) ◽  
pp. 455-463 ◽  
Author(s):  
Shinjiro Ogita ◽  
Miki Usui ◽  
Nanae Shibutani ◽  
Yasuo Kato
Keyword(s):  
Particle Bombardment ◽  
Shoot Multiplication ◽  
Agrobacterium Mediated Transformation ◽  
Internode Explants

A simple and effective method for protein subcellular localization using Agrobacterium-mediated transformation of onion epidermal cells

Biologia ◽  
2007 ◽  
Vol 62 (5) ◽  
Author(s):  
Wei Sun ◽  
Ziyi Cao ◽  
Yan Li ◽  
Yanxiu Zhao ◽  
Hui Zhang
Keyword(s):  
Subcellular Localization ◽  
Particle Bombardment ◽  
Fusion Proteins ◽  
Fluorescent Proteins ◽  
Transformed Cells ◽  
Protein Subcellular Localization ◽  
Transformation Protocol ◽  
Agrobacterium Mediated Transformation ◽  
The Mean ◽  
Onion Epidermal Cells

AbstractA modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5±0.9%, while that of the particle bombardment method is at the range 2.0±0.4%. To compare with the prevailing method of micro-projectile bombardment, the modified Agrobacterium-mediated transformation may provide with higher efficiency and even more simplified manipulability on a lower budget.

Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation

2004 ◽  
Vol 13 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Huixia Shou ◽  
Bronwyn R. Frame ◽  
Steven A. Whitham ◽  
Kan Wang
Keyword(s):  
Particle Bombardment ◽  
Transgenic Maize ◽  
Agrobacterium Mediated Transformation

scholarly journals A Dark Incubation Period Is Important for Agrobacterium-Mediated Transformation of Mature Internode Explants of Sweet Orange, Grapefruit, Citron, and a Citrange Rootstock

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47426 ◽  
Author(s):  
Mizuri Marutani-Hert ◽  
Kim D. Bowman ◽  
Greg T. McCollum ◽  
T. Erik Mirkov ◽  
Terence J. Evens ◽  
...  
Keyword(s):  
Incubation Period ◽  
Sweet Orange ◽  
Dark Incubation ◽  
Agrobacterium Mediated Transformation ◽  
Internode Explants

Agrobacterium-Mediated Transformation Of Plant Cells

2018 ◽  
Author(s):  
Christine Kronfoth ◽  
◽  
Peter Grayson ◽  
Keyword(s):  
Plant Cells ◽  
Agrobacterium Mediated Transformation
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