Functional characterization of a geraniol synthase-encoding gene from Camptotheca acuminata and its application in production of geraniol in Escherichia coli

2016 ◽  
Vol 43 (9) ◽  
pp. 1281-1292 ◽  
Author(s):  
Fei Chen ◽  
Wei Li ◽  
Liangzhen Jiang ◽  
Xiang Pu ◽  
Yun Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Characterization ◽  
Camptotheca Acuminata ◽  
Geraniol Synthase ◽  
Encoding Gene

scholarly journals N-Acetylglucosamine-1-Phosphate Transferase, WecA, as a Validated Drug Target in Mycobacterium tuberculosis

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Stanislav Huszár ◽  
Vinayak Singh ◽  
Alica Polčicová ◽  
Peter Baráth ◽  
María Belén Barrio ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Target ◽  
Functional Characterization ◽  
Drug Candidate ◽  
Content Type ◽  
Chemical Libraries ◽  
Whole Cells ◽  
Encoding Gene

ABSTRACT The mycobacterial phosphoglycosyltransferase WecA, which initiates arabinogalactan biosynthesis in Mycobacterium tuberculosis, has been proposed as a target of the caprazamycin derivative CPZEN-45, a preclinical drug candidate for the treatment of tuberculosis. In this report, we describe the functional characterization of mycobacterial WecA and confirm the essentiality of its encoding gene in M. tuberculosis by demonstrating that the transcriptional silencing of wecA is bactericidal in vitro and in macrophages. Silencing wecA also conferred hypersensitivity of M. tuberculosis to the drug tunicamycin, confirming its target selectivity for WecA in whole cells. Simple radiometric assays performed with mycobacterial membranes and commercially available substrates allowed chemical validation of other putative WecA inhibitors and resolved their selectivity toward WecA versus another attractive cell wall target, translocase I, which catalyzes the first membrane step in the biosynthesis of peptidoglycan. These assays and the mutant strain described herein will be useful for identifying potential antitubercular leads by screening chemical libraries for novel WecA inhibitors.

In vitro functional characterization of overproduced Escherichia coli katF/rpoS gene product

Biochemistry ◽  
1993 ◽  
Vol 32 (41) ◽  
pp. 11112-11117 ◽  
Author(s):  
Lam H. Nguyen ◽  
Debra B. Jensen ◽  
Nancy E. Thompson ◽  
Daniel R. Gentry ◽  
Richard R. Burgess
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Functional Characterization ◽  
Rpos Gene
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