White root rot disease of the lacquer tree Toxicodendron vernicifluum caused by Rosellinia necatrix

2011 ◽  
Vol 78 (1) ◽  
pp. 77-79 ◽  
Author(s):  
Shuhei Takemoto ◽  
Hitoshi Nakamura ◽  
Masanobu Tabata ◽  
Atsuko Sasaki ◽  
Yu Ichihara ◽  
...  
Keyword(s):  
Root Rot ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Rot Disease ◽  
Toxicodendron Vernicifluum

scholarly journals Response of the Biocontrol AgentPseudomonas pseudoalcaligenesAVO110 toRosellinia necatrixExudate

2018 ◽  
Vol 85 (3) ◽  
Author(s):  
Clara Pliego ◽  
José Ignacio Crespo-Gómez ◽  
Adrián Pintado ◽  
Isabel Pérez-Martínez ◽  
Antonio de Vicente ◽  
...  
Keyword(s):  
Root Rot ◽  
Molecular Mechanisms ◽  
Dna Helicase ◽  
Root Tip ◽  
Rosellinia Necatrix ◽  
Pseudomonas Pseudoalcaligenes ◽  
White Root Rot ◽  
Content Type ◽  
Root Rot Disease ◽  
Rot Disease

ABSTRACTThe rhizobacteriumPseudomonas pseudoalcaligenesAVO110, isolated by the enrichment of competitive avocado root tip colonizers, controls avocado white root rot disease caused byRosellinia necatrix. Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudate-containing medium with the goal of identifying the molecular mechanisms linked to the interaction of this bacterium withR. necatrix. A total of 26 STM mutants outcompeted by the parental strain in fungal exudate, but not in rich medium, were selected and namedgrowth-attenuatedmutants (GAMs). Twenty-one genes were identified as being required for this bacterial-fungal interaction, including membrane transporters, transcriptional regulators, and genes related to the metabolism of hydrocarbons, amino acids, fatty acids, and aromatic compounds. The bacterial traits identified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) or cyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase (recB) and a regulator of alginate production (algQ) were identified as being required for efficient colonization of the avocado rhizosphere.IMPORTANCEDiseases associated with fungal root invasion cause a significant loss of fruit tree production worldwide. The bacteriumPseudomonas pseudoalcaligenesAVO110 controls avocado white root rot disease caused byRosellinia necatrixby using mechanisms involving competition for nutrients and niches. Here, a functional genomics approach was conducted to identify the bacterial traits involved in the interaction with this fungal pathogen. Our results contribute to a better understanding of the multitrophic interactions established among bacterial biocontrol agents, the plant rhizosphere, and the mycelia of soilborne pathogens.

scholarly journals Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis

2019 ◽  
Author(s):  
Adela Zumaquero ◽  
Satoko Kanematsu ◽  
Hitoshi Nakayashiki ◽  
Antonio Matas ◽  
Elsa Martínez-Ferri ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Root Rot ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Infection Process ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Host Interaction ◽  
Rot Disease

Abstract Background White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado `Dusa´ roots with that obtained from the fungus cultured in rich medium. Results The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1,937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. Conclusions The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

scholarly journals Genetic Diversity and Biocontrol of Rosellinia necatrix Infecting Apple in Northern Italy

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 444-452 ◽  
Author(s):  
Luca Pasini ◽  
Daniele Prodorutti ◽  
Sandro Pastorelli ◽  
Ilaria Pertot
Keyword(s):  
Root Rot ◽  
Biocontrol Agent ◽  
Organic Fertilizers ◽  
Trichoderma Atroviride ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Wood Residues ◽  
Main Factors ◽  
Rot Disease

The soilborne fungus Rosellinia necatrix is the causal agent of white root rot disease on numerous plant species, including apple, which, together with the ability to survive in soil for long periods, makes this pathogen difficult to control. To understand the origins of pathogen infestation, a survey of diseased apple orchards in the northeast of Italy was conducted and 35 isolates of R. necatrix were characterized with intersimple sequence repeat markers. High genetic heterogeneity among the collected isolates suggested multiple preexisting sources of inoculum and not movement of infected soil or plant material from a single source. Greenhouse trials confirmed that, as with some other crops, soil water content and temperature were the main factors influencing infection of apple plants, while organic fertilizers and the incorporation of apple wood residues were less important. The efficacy of Trichoderma atroviride SC1 as a biocontrol agent against R. necatrix greatly depended on the timing of application. It reduced white root rot incidence on apple seedlings only if treatment was applied at least 1 week before planting.

scholarly journals Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
A. Zumaquero ◽  
S. Kanematsu ◽  
H. Nakayashiki ◽  
A. Matas ◽  
E. Martínez-Ferri ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Root Rot ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Infection Process ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Host Interaction ◽  
Rot Disease

Abstract Background White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado ‘Dusa’ roots with that obtained from the fungus cultured in rich medium. Results The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. Conclusions The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

scholarly journals Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis

2019 ◽  
Author(s):  
Adela Zumaquero ◽  
Satoko Kanematsu ◽  
Hitoshi Nakayashiki ◽  
Antonio Matas ◽  
Elsa Martínez-Ferri ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Root Rot ◽  
Plant Cell Wall ◽  
Infection Process ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Host Interaction ◽  
Wide Range ◽  
Rot Disease

Abstract Background White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado `Dusa´ roots with that obtained from the fungus cultured in rich medium. Results The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1,937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. Conclusions The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of a wide range of compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among different plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

scholarly journals Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis

2019 ◽  
Author(s):  
Adela Zumaquero ◽  
Satoko Kanematsu ◽  
Hitoshi Nakayashiki ◽  
Antonio Matas ◽  
Elsa Martínez-Ferri ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Root Rot ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Infection Process ◽  
Rosellinia Necatrix ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Host Interaction ◽  
Rot Disease

Abstract Background White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado `Dusa´ roots with that obtained from the fungus cultured in rich medium. Results The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1,937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. Conclusions The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

White root rot disease suppression in rubber plant with microbial co-inoculants and silicon addition

Rhizosphere ◽  
2020 ◽  
Vol 15 ◽  
pp. 100221
Author(s):  
Imran Shabbir ◽  
Mohd Yusoff Abd Samad ◽  
Radziah Othman ◽  
Mui-Yun Wong ◽  
Zulkefly Sulaiman ◽  
...  
Keyword(s):  
Root Rot ◽  
Disease Suppression ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Rubber Plant ◽  
Silicon Addition ◽  
Rot Disease

scholarly journals An Assay On Potential of Local Trichoderma Spp. to Control White Root Rot Disease Caused by Rigidoporus Microporus in Rubber Plant Stump

2017 ◽  
Vol 11 (2) ◽  
pp. 717-723
Author(s):  
Dwi Suryanto ◽  
Rani Artha Munthe ◽  
Isnaini Nurwahyuni ◽  
Erman Munir
Keyword(s):  
Root Rot ◽  
Trichoderma Spp ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Rubber Plant ◽  
Rot Disease
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