An Assay On Potential of Local Trichoderma Spp. to Control White Root Rot Disease Caused by Rigidoporus Microporus in Rubber Plant Stump

The preventive control of white root rot disease in small holder plantation using botanical, biological, and chemical agents. A field and laboratory experiment were conducted from June 2008 to December 2009 in Panumangan, Tulang Bawang - Lampung. The field experiment was intended to evaluate the effect of botanical plants (Alpinia galanga, Sansiviera auranthii, and Marantha arundinacea), biological agents (organic matter and Trichoderma spp.), and chemical agents (lime and natural sulphur) on the incidence of white root rot disease and population of some soil microbes. The laboratory experiment was conducted to observe the mechanism of botanical agents in controlling white root rot disease. In the field experiment, the treatments were applied in the experimental plot with cassava plant infection as the indicator. The variables examined were the incidence of white root rot and population of soil microbes. In the laboratory experiment, culture of R. microporus was grown in PDA containing root exudate of the antagonistic plant (botanical agent). The variable examined was colony diameter of R. microporus growing in the PDA plates. The results of the field experiment showed that planting of the botanical agents, and application of Trichoderma spp., as well as natural sulphur, decreased the incidence of white root rot disease. The effectiveness of M. arundinacea and Trichoderma spp. was comparable to natural sulphur. The laboratory experiment showed only root exudate of A. galanga and S. auranthii that were significantly inhibit the growth of R. microporus.

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Response of the Biocontrol AgentPseudomonas pseudoalcaligenesAVO110 toRosellinia necatrixExudate

Applied and Environmental Microbiology ◽

10.1128/aem.01741-18 ◽

2018 ◽

Vol 85 (3) ◽

Author(s):

Clara Pliego ◽

José Ignacio Crespo-Gómez ◽

Adrián Pintado ◽

Isabel Pérez-Martínez ◽

Antonio de Vicente ◽

...

Keyword(s):

Root Rot ◽

Molecular Mechanisms ◽

Dna Helicase ◽

Root Tip ◽

Rosellinia Necatrix ◽

Pseudomonas Pseudoalcaligenes ◽

White Root Rot ◽

Content Type ◽

Root Rot Disease ◽

Rot Disease

ABSTRACTThe rhizobacteriumPseudomonas pseudoalcaligenesAVO110, isolated by the enrichment of competitive avocado root tip colonizers, controls avocado white root rot disease caused byRosellinia necatrix. Here, we applied signature-tagged mutagenesis (STM) during the growth and survival of AVO110 in fungal exudate-containing medium with the goal of identifying the molecular mechanisms linked to the interaction of this bacterium withR. necatrix. A total of 26 STM mutants outcompeted by the parental strain in fungal exudate, but not in rich medium, were selected and namedgrowth-attenuatedmutants (GAMs). Twenty-one genes were identified as being required for this bacterial-fungal interaction, including membrane transporters, transcriptional regulators, and genes related to the metabolism of hydrocarbons, amino acids, fatty acids, and aromatic compounds. The bacterial traits identified here that are involved in the colonization of fungal hyphae include proteins involved in membrane maintenance (a dynamin-like protein and ColS) or cyclic-di-GMP signaling and chemotaxis. In addition, genes encoding a DNA helicase (recB) and a regulator of alginate production (algQ) were identified as being required for efficient colonization of the avocado rhizosphere.IMPORTANCEDiseases associated with fungal root invasion cause a significant loss of fruit tree production worldwide. The bacteriumPseudomonas pseudoalcaligenesAVO110 controls avocado white root rot disease caused byRosellinia necatrixby using mechanisms involving competition for nutrients and niches. Here, a functional genomics approach was conducted to identify the bacterial traits involved in the interaction with this fungal pathogen. Our results contribute to a better understanding of the multitrophic interactions established among bacterial biocontrol agents, the plant rhizosphere, and the mycelia of soilborne pathogens.

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Host Range ofDematophora necatrix,the Cause of White Root Rot Disease in Fruit Trees

Plant Disease ◽

10.1094/pd-64-662 ◽

1980 ◽

Vol 64 (7) ◽

pp. 662 ◽

Cited By ~ 56

Author(s):

A. Sztejnberg

Keyword(s):

Host Range ◽

Root Rot ◽

Fruit Trees ◽

White Root Rot ◽

Root Rot Disease ◽

Rot Disease

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Role of Bradyrhizobium japonicum and Trichoderma spp. in the control of root rot disease of soybean

Acta Mycologica ◽

10.5586/am.1995.003 ◽

2014 ◽

Vol 30 (1) ◽

pp. 35-40 ◽

Cited By ~ 1

Author(s):

Syed Ehteshamul-Haque ◽

Abdul Ghaffar

Keyword(s):

Grain Yield ◽

Rhizoctonia Solani ◽

Trichoderma Harzianum ◽

Root Rot ◽

Bradyrhizobium Japonicum ◽

Seed Treatment ◽

Trichoderma Spp ◽

Root Rot Disease ◽

Rot Disease

Seed treatment of soybean with Bndyrhizobium japonicum, Trichoderma harzianum, T. viride, T. hamatum, T. koningii and T. pseudokoningii significantly controlled the infection of 30-day-old seedlingsby Maerophomina phaseolina, Rhizoctonia solani and Fusarium spp. In 60-day-old plants Trichoderma spp.. and B. japonicum inhibited the grouth of R. solani and Fusarium spp., whereas the use of B. japonicum (TAL-102) with T. harzianum. T. viride, T. koningii and T. pseudokoningii controlled the infection by M. phaseolina. Greater grain yield was recorded when B. japonium (TAI-102) was used with T. hamatum.

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Transcriptome analysis of the fungal pathogen Rosellinia necatrix during infection of a susceptible avocado rootstock identifies potential mechanisms of pathogenesis

10.21203/rs.2.12746/v2 ◽

2019 ◽

Author(s):

Adela Zumaquero ◽

Satoko Kanematsu ◽

Hitoshi Nakayashiki ◽

Antonio Matas ◽

Elsa Martínez-Ferri ◽

...

Keyword(s):

Transcriptome Analysis ◽

Root Rot ◽

Molecular Mechanisms ◽

Plant Cell Wall ◽

Infection Process ◽

Rosellinia Necatrix ◽

White Root Rot ◽

Root Rot Disease ◽

Host Interaction ◽

Rot Disease

Abstract Background White root rot disease caused by Rosellinia necatrix is one of the most important threats affecting avocado productivity in tropical and subtropical climates. Control of this disease is complex and nowadays, lies in the use of physical and chemical methods, although none have proven to be fully effective. Detailed understanding of the molecular mechanisms underlying white root rot disease has the potential of aiding future developments in disease resistance and management. In this regard, this study used RNA-Seq technology to compare the transcriptomic profiles of R. necatrix during infection of susceptible avocado `Dusa´ roots with that obtained from the fungus cultured in rich medium. Results The transcriptomes from three biological replicates of R. necatrix colonizing avocado roots (RGA) and R. necatrix growing on potato dextrose agar media (RGPDA) were analyzed using Illumina sequencing. A total of 12,104 transcripts were obtained, among which 1,937 were differentially expressed genes (DEG), 137 exclusively expressed in RGA and 160 in RGPDA. During the root infection process, genes involved in the production of fungal toxins, detoxification and transport of toxic compounds, hormone biosynthesis, gene silencing and plant cell wall degradation were overexpressed. Interestingly, 24 out of the 137 contigs expressed only during R. necatrix growth on avocado roots, were predicted as candidate effector proteins (CEP) with a probability above 60%. The PHI (Pathogen Host Interaction) database revealed that three of the R. necatrix CEP showed homology with previously annotated effectors, already proven experimentally via pathogen-host interaction. Conclusions The analysis of the full-length transcriptome of R. necatrix during the infection process is suggesting that the success of this fungus to infect roots of diverse crops might be attributed to the production of different compounds which, singly or in combination, interfere with defense or signaling mechanisms shared among distinct plant families. The transcriptome analysis of R. necatrix during the infection process provides useful information and facilitates further research to a more in -depth understanding of the biology and virulence of this emergent pathogen. In turn, this will make possible to evolve novel strategies for white root rot management in avocado.

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