Feasibility of intravoxel incoherent motion in the assessment of tumor microvasculature and blood–brain barrier integrity: a case-based evaluation of gliomas

2021 ◽  
Author(s):  
Andre Monteiro Paschoal ◽  
Maria Clara Zanon Zotin ◽  
Lucas Murilo da Costa ◽  
Antonio Carlos dos Santos ◽  
Renata Ferranti Leoni
Keyword(s):  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Intravoxel Incoherent Motion ◽  
Barrier Integrity ◽  
Tumor Microvasculature ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity ◽  
Case Based

scholarly journals AIM2 deletion enhances blood‐brain barrier integrity in experimental ischemic stroke

2021 ◽  
Author(s):  
Si‐yi Xu ◽  
Hui‐jie Bian ◽  
Shu Shu ◽  
Sheng‐nan Xia ◽  
Yue Gu ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

scholarly journals Sex differences in CSF biomarkers for neurodegeneration and blood‐brain barrier integrity

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Tobias Skillbäck ◽  
Kaj Blennow ◽  
Henrik Zetterberg ◽  
Sara Shams ◽  
Alejandra Machado ◽  
...  
Keyword(s):  
Sex Differences ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Csf Biomarkers ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

scholarly journals A novel multi-frequency trans-endothelial electrical resistance (MTEER) sensor array to monitor blood-brain barrier integrity

2021 ◽  
Vol 334 ◽  
pp. 129599
Author(s):  
Maider Badiola-Mateos ◽  
Davide Di Giuseppe ◽  
Roberto Paoli ◽  
Maria Jose Lopez-Martinez ◽  
Arianna Mencattini ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Electrical Resistance ◽  
Sensor Array ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

Abstract TP256: Mouse Model of Uremic Cerebral Microbleeds

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Wei Ling Lau ◽  
Mary Tarbiat-Boldaji ◽  
Hayley Smalls ◽  
Ane Nunes ◽  
Javad Savoj ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tight Junction ◽  
Blood Brain Barrier ◽  
Cerebral Microbleeds ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Increased Risk ◽  
Junction Protein ◽  
Blood Brain Barrier Integrity

Introduction: Cerebral microbleeds are more common in chronic kidney disease (CKD) and dialysis patients compared to the general population. Diminished kidney function alone appears to be a risk factor for microbleeds, independent of age and hypertension. Microbleed burden in CKD patients is associated with increased risk of future hemorrhagic stroke and with cognitive dysfunction. The mechanisms that drive uremic microbleed formation are unclear. Hypothesis: We hypothesized that CKD mice are predisposed to develop cerebral microhemorrhages (the pathologic substrate of microbleeds), and that a standardized inflammatory stimulus (lipopolysaccharide, LPS) will amplify microhemorrhage burden in CKD mice compared to non-CKD controls (CTL). We also hypothesized that uremia induces depletion of tight junction proteins, altering blood-brain barrier integrity and representing a potential mechanism of microbleed formation. Methods: Animal groups included CTL (n=3), CKD (n=3), CTL+LPS (n=5) and CKD+LPS (n=5). CKD induction in male C57BL/6 mice was achieved via nephrotoxic adenine diet x18 days. Two weeks following CKD induction, CKD and control mice were treated with LPS 1 mg/kg i.p. dosed at 0, 6 and 24 hours. Brains were harvested one week after LPS injections and 40-micron sections were stained using Prussian blue to identify microhemorrhages. Immunohistochemistry was performed for the blood-brain barrier tight junction protein claudin-5. Results: CKD mice had significantly elevated blood urea nitrogen, and tubulointerstitial fibrosis was present on kidney histology. Total number of microhemorrhages per brain was 2.3±1.5 (mean ± standard error of the mean) for CTL mice, 8.3±1.5 for CKD mice, 23.2±4.2 for CTL+LPS mice, and 27.6±6.2 for CKD+LPS mice (p<0.05 for CKD+LPS vs. CTL). Immunostaining showed decreased claudin-5 expression in CKD mice compared to CTL. Conclusions: We have generated a mouse model that will facilitate future mechanistic studies in the field of uremic microbleeds. Our initial findings suggest that CKD alters blood-brain barrier integrity and that inflammation amplifies development of microbleeds in CKD.

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