A 3D tubular structure with droplet generation and temperature control for DNA amplification

2021 ◽  
Vol 25 (7) ◽  
Author(s):  
Shaw-Hwa Parng ◽  
Ping-Jung Wu ◽  
Yu-Yin Tsai ◽  
Ruey-Shyan Hong ◽  
Su-Jan Lee
Keyword(s):  
Temperature Control ◽  
Dna Amplification ◽  
Tubular Structure ◽  
Droplet Generation

SIZE-CONTROLLED DROPLET GENERATION IN A MICROFLUIDIC DEVICE FOR RARE DNA AMPLIFICATION BY OPTIMIZING ITS EFFECTIVE PARAMETERS

2018 ◽  
Vol 18 (01) ◽  
pp. 1850002 ◽  
Author(s):  
ALI LASHKARIPOUR ◽  
ALI ABOUEI MEHRIZI ◽  
MASOUD GOHARIMANESH ◽  
MOHAMMADREZA RASOULI ◽  
SAJAD RAZAVI BAZAZ
Keyword(s):  
Single Molecule ◽  
Dominant Role ◽  
Dna Amplification ◽  
Microfluidic Devices ◽  
Sample Volume ◽  
Droplet Generation ◽  
Droplet Radius ◽  
Generation Process ◽  
Effective Parameters ◽  
Precise Control

Versatility and portability of microfluidic devices play a dominant role in their widespread use by researchers. Droplet-based microfluidic devices have been extensively used due to their precise control over sample volume, and ease of manipulating and addressing each droplet on demand. Droplet-based polymerase chain reaction (PCR) devices are particularly desirable in single DNA amplification. If the droplets are small enough to contain only one DNA molecule, single molecule amplification becomes possible, which can be advantageous in several cases such as early cancer detection. In this work, flow-focusing microfluidic droplet generation’s parameters are numerically investigated and optimized for generating the smallest droplet possible, while considering fabrication limits. Taguchi design of experiment method is used to study the effects of key parameters in droplet generation. By exploiting this approach, a droplet with a radius of 111[Formula: see text]nm is generated using a 3[Formula: see text][Formula: see text]m orifice. Since the governing physics of the droplet generation process is not totally understood yet, by means of analysis of variance (ANOVA) analysis, a generalized linear model (GLM) is proposed to predict the droplet radius, given the values of eight major parameters affecting the droplet size. The proposed model shows a correlation of 95.3% and 64.95% for droplets of radius greater than and lower than 5[Formula: see text][Formula: see text]m, respectively. Finally, the source of this variation of behavior in different size scales is identified.

A Disposable Polydimethylsiloxane Microdevice for DNA Amplification

2010 ◽  
Vol 93-94 ◽  
pp. 105-108 ◽  
Author(s):  
A. Lekwichai ◽  
S. Porntheeraphat ◽  
Win Bunjongpru ◽  
W. Sripumkhai ◽  
J. Supadech ◽  
...  
Keyword(s):  
Thin Film ◽  
Temperature Control ◽  
Mass Production ◽  
Temperature Sensor ◽  
Dna Amplification ◽  
The Other ◽  
Si Substrate ◽  
Mixture Volume ◽  
Cmos Compatible ◽  
Pdms Microchip

In this study, we demonstrate the disposable polydimethylsiloxane (PDMS) microchip provided for DNA amplification. The device consists of two main parts. The first part is PDMS/glass stationary chamber, the other part is a temperature-control microdevice on SiO2/Si substrate. This device consists of a thin film Pt-microheater and a Pt-temperature sensor, which were fabricated with CMOS compatible process. The performance of the device in the DNA amplification shows that, with 10 μl of PCR mixture volume, the approximately 700 bp DNA were successfully amplified within 50 minutes by 30 PCR cycles. The amplified products were comparable with those of a conventional method using electrophoresis. The PCR chip is also suitable for mass production.

MEMS-based Temperature Control Systems for DNA Amplification

2002 ◽  
Vol 3 (3-4) ◽  
Author(s):  
Chia-Yen Lee ◽  
Gwo-Bin Lee ◽  
Heng-Hui Liu ◽  
Fu-Chun Huang
Keyword(s):  
Control Systems ◽  
Temperature Control ◽  
Dna Amplification

A High Angle, Double Tilting Cold Stage for the AEI EM7

1974 ◽  
Vol 32 ◽  
pp. 450-451
Author(s):  
P.R. Swann ◽  
A.E. Lloyd
Keyword(s):  
Habit Plane ◽  
Temperature Control ◽  
Tilt Angle ◽  
Cooling System ◽  
Thermal Drift ◽  
High Angle ◽  
Cold Stage ◽  
High Degree ◽  
Better Than

Figure 1 shows the design of a specimen stage used for the in situ observation of phase transformations in the temperature range between ambient and −160°C. The design has the following features a high degree of specimen stability during tilting linear tilt actuation about two orthogonal axes for accurate control of tilt angle read-out high angle tilt range for stereo work and habit plane determination simple, robust construction temperature control of better than ±0.5°C minimum thermal drift and transmission of vibration from the cooling system.

Behavioral Temperature Control

1981 ◽  
Vol 26 (8) ◽  
pp. 636-637
Author(s):  
James M. Lipton
Keyword(s):  
Temperature Control

INVESTIGATING THE USE OF PHASE-CHANGE MATERIALS FOR TEMPERATURE CONTROL DURING FAST FILLING OF HYDROGEN CYLINDERS

2018 ◽  
Vol 22 (2-3) ◽  
pp. 73-97
Author(s):  
Vishagen Ramasamy ◽  
Edward S. Richardson ◽  
Philippa Reed ◽  
Warren Hepples ◽  
Andrew Wheeler
Keyword(s):  
Phase Change ◽  
Temperature Control ◽  
Phase Change Materials
Sign in / Sign up

Export Citation Format

Share Document