Immune responses and protective efficacy of outer membrane protein ExbB of Pseudomonas fluorescens against Aeromonas hydrophila and Pseudomonas fluorescens affecting Carassius auratus

Aeromonas hyrophila strains AHV1, AHV2 and AH3 were isolated and identified from Muscle tissue, intestine, body fluid and gills of infected gold fish Carassius auratus. In order to study their virulence, LD50 tests against normal gold fish, proteolytic, haemolytic and challenge studies were performed. The virulence studies revealed that, both AHV1 and AHV2 strains are highly positive for proteolytic and haemolytic properties. The LD50 data showed that, the fish C. auratus are highly susceptible to A. hyrophila strains AHV1 and AHV2 at cent percent lethal rate. The survival of C. auratus significantly (P<0.05) decreased when challenged with virulent strains of A. hydrophila AHV1 and AHV2. The outer membrane protein (OMP-TS) gene was successfully amplified generating an the amplicon size of 1008 bp. The amplified product from the genomic DNA of AHV1 strain was cloned in to pTZ57R/T vector, transformed into DH5? cells and sequenced. The sequenced clone is resembling to various A. hydrophila isolates and submitted to NCBI GenBank database (accession no:HQ331525). DOI: http://dx.doi.org/10.3329/bjm.v28i2.11819 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 70-75

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A Recombinant Chlamydia trachomatis MOMP Vaccine Elicits Cross-serogroup Protection in Mice Against Vaginal Shedding and Infertility

The Journal of Infectious Diseases ◽

10.1093/infdis/jiz438 ◽

2019 ◽

Vol 221 (2) ◽

pp. 191-200 ◽

Cited By ~ 2

Author(s):

Delia F Tifrea ◽

Sukumar Pal ◽

Luis M de la Maza

Keyword(s):

Chlamydia Trachomatis ◽

Membrane Protein ◽

Immune Responses ◽

Outer Membrane ◽

Outer Membrane Protein ◽

Subunit Vaccine ◽

Bacterial Pathogen ◽

Male Mice ◽

Fertility Rates ◽

Sexually Transmitted

Abstract Background Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen worldwide. Here, we determined the ability of a C. trachomatis recombinant major outer membrane protein (rMOMP) vaccine to elicit cross-serogroup protection. Methods Female C3H/HeN mice were vaccinated by mucosal and systemic routes with C. trachomatis serovar D (UW-3/Cx) rMOMP and challenged in the ovarian bursa with serovars D (UW-3/Cx), D (UCI-96/Cx), E (IOL-43), or F (N.I.1). CpG-1826 and Montanide ISA 720 were used as adjuvants. Results Immune responses following vaccination were more robust against the most closely related serovars. Following a genital challenge (as determined by number of mice with positive vaginal cultures, number of positive cultures, number of inclusion forming units recovered, and number of days with positive cultures) mice challenged with C. trachomatis serovars of the same complex were protected but not those challenged with serovar F (N.I.1) from a different subcomplex. Females were caged with male mice. Based on fertility rates, number of embryos, and hydrosalpinx formation, vaccinated mice were protected against challenges with serovars D (UW-3/Cx), D (UCI-96/Cx), and E (IOL-43) but not F (N.I.1). Conclusions This is the first subunit vaccine shown to protect mice against infection, pathology, and infertility caused by different C. trachomatis serovars.

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