Resazurin Dye as a Reliable Tool for Determination of Cell Number and Viability in Mesenchymal Stem Cell Culture

2010 ◽  
Vol 150 (1) ◽  
pp. 157-159 ◽  
Author(s):  
T. Dienstknecht ◽  
K. Ehehalt ◽  
Zsuzsa Jenei-Lanzl ◽  
J. Zellner ◽  
Michael Müller ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Number ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture ◽  
Reliable Tool ◽  
Resazurin Dye

scholarly journals Re-using blood products as an alternative supplement in the optimisation of clinical-grade adipose-derived mesenchymal stem cell culture

2017 ◽  
Vol 6 (7) ◽  
pp. 414-422 ◽  
Author(s):  
J. Phetfong ◽  
T. Tawonsawatruk ◽  
K. Seenprachawong ◽  
A. Srisarin ◽  
C. Isarankura-Na-Ayudhya ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Clinical Grade ◽  
Blood Products ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture

scholarly journals A new method to confirm the absence of human and animal serum in mesenchymal stem cell culture media

2019 ◽  
Vol 16 (8) ◽  
pp. 1102-1106 ◽  
Author(s):  
Megumi Ota ◽  
Kentaro Takagaki ◽  
Sho Takaoka ◽  
Hideki Tanemura ◽  
Naoki Urushihata
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Culture Media ◽  
New Method ◽  
Cell Culture Media ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture

scholarly journals Feasibility of mesenchymal stem cell culture expansion for a phase I clinical trial in multiple sclerosis

2018 ◽  
Vol 4 (1) ◽  
pp. 205521731876528 ◽  
Author(s):  
Sarah M Planchon ◽  
Karen T Lingas ◽  
Jane Reese Koç ◽  
Brittney M Hooper ◽  
Basabi Maitra ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Phase I ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture ◽  
Multiple Sclerosis Patients

Background Multiple sclerosis is an inflammatory, neurodegenerative disease of the central nervous system for which therapeutic mesenchymal stem cell transplantation is under study. Published experience of culture-expanding multiple sclerosis patients’ mesenchymal stem cells for clinical trials is limited. Objective To determine the feasibility of culture-expanding multiple sclerosis patients’ mesenchymal stem cells for clinical use. Methods In a phase I trial, autologous, bone marrow-derived mesenchymal stem cells were isolated from 25 trial participants with multiple sclerosis and eight matched controls, and culture-expanded to a target single dose of 1–2 × 106 cells/kg. Viability, cell product identity and sterility were assessed prior to infusion. Cytogenetic stability was assessed by single nucleotide polymorphism analysis of mesenchymal stem cells from 18 multiple sclerosis patients and five controls. Results One patient failed screening. Mesenchymal stem cell culture expansion was successful for 24 of 25 multiple sclerosis patients and six of eight controls. The target dose was achieved in 16–62 days, requiring two to three cell passages. Growth rate and culture success did not correlate with demographic or multiple sclerosis disease characteristics. Cytogenetic studies identified changes on one chromosome of one control (4.3%) after extended time in culture. Conclusion Culture expansion of mesenchymal stem cells from multiple sclerosis patients as donors is feasible. However, culture time should be minimized for cell products designated for therapeutic administration.

scholarly journals Polydopamine and collagen coated micro-grated polydimethylsiloxane for human mesenchymal stem cell culture

2019 ◽  
Vol 4 ◽  
pp. 142-150 ◽  
Author(s):  
Dhavan Sharma ◽  
Wenkai Jia ◽  
Fei Long ◽  
Shweta Pati ◽  
Qinghui Chen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture

Impact of starting material (fresh versus cryopreserved marrow) on mesenchymal stem cell culture

Transfusion ◽  
2017 ◽  
Vol 57 (9) ◽  
pp. 2216-2219 ◽  
Author(s):  
Alesia Kaplan ◽  
Katie Sackett ◽  
Darin Sumstad ◽  
Dianne Kadidlo ◽  
David H. McKenna
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Starting Material ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture
Sign in / Sign up

Export Citation Format

Share Document