Association of STAT5A Gene Variants with Milk Production Traits in Agerolese Cattle

The objective of this study was to obtain genetic marker information in the Gyr breed by analyzing bGH and Pit-1 gene polymorphisms and to verify their association with milk production traits. One sample including 40 Gyr bulls were genotyped at two bGH gene restriction sites (bGH- AluI and bGH-MspI) and at one restriction site in the Pit-1 gene (Pit-1 HinfI). The bGH-MspI(-) allele was favorable for fat milk percentage. The heterozigous Pit-1 HinfI (+/-) bulls were superior for fat milk production, in relation to homozigous Pit-1 HinfI (+/+). The Pit-1 and bGH genes are strong candidates in the dairy cattle QTL search, and zebuine populations are promising samples for this purpose.

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A Study on the Effect of Prolactin Gene Variants on Milk Production Traits of Holstein Cattle

Russian Journal of Genetics ◽

10.1134/s1022795419040082 ◽

2019 ◽

Vol 55 (4) ◽

pp. 480-486 ◽

Cited By ~ 1

Author(s):

S. Bayıl Oğuzkan ◽

A. S. Bozkurt

Keyword(s):

Milk Production ◽

Holstein Cattle ◽

Gene Variants ◽

Production Traits ◽

Milk Production Traits ◽

Prolactin Gene

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Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle

Journal of Dairy Research ◽

10.1017/s0022029909990070 ◽

2009 ◽

Vol 76 (4) ◽

pp. 441-445 ◽

Cited By ~ 20

Author(s):

Maria Selvaggi ◽

Cataldo Dario ◽

Giovanni Normanno ◽

Gaetano V Celano ◽

Marco Dario

Keyword(s):

Statistical Analysis ◽

Milk Production ◽

Farm Animals ◽

Target Cells ◽

Production Traits ◽

Milk Production Traits ◽

Protein Percentage ◽

Significant Difference ◽

Brown Cattle ◽

Stat5a Gene

STATs are a group of transcription factors that mediate actions of a variety of peptide hormones and cytokines within target cells (for example, prolactin and growth hormone). Therefore, STAT5A gene is a candidate marker for quantitative traits in farm animals with respect to milk production traits. In this study the STAT5A/AvaI polymorphism was investigated with PCR-RFLP in a sample of 233 Italian Brown cattle. This polymorphism is localized in the coding region of the bovine STAT5A gene. It is a substitution C→T at position 6853 within exon 7. All three possible genotypes for the C/T polymorphism were identified. The overall frequencies of alleles C and T were 0·83 and 0·17 respectively; the Hardy-Weinberg equilibrium was verified. In order to study the relationship between STAT5A/AvaI polymorphism and milk performance traits, the data for a 305-d milk production that included milk yield, protein and fat yield, fat and protein percentage were used. Significant differences between the two genotypes were found in yields of milk, fat and protein and protein percentage (P<0·01). CC cows produced more milk than CT (5418·68 v. 5149·54 kg). Protein content was higher in milk from CC compared with CT genotypes (3·40 v. 3·21%). No significant difference was found in fat content. Owing to the low number of TT cows in the studied population, this genotype was not included in the statistical analysis; in fact the number of TT cows was not enough to provide an accurate statistical analysis. Although more studies are needed to better clarify the role of this SNP on production traits, STAT5A/AvaI polymorphism appears to be a promising indirect marker to improve milk production traits in cattle.

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Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits

Czech Journal of Animal Science ◽

10.17221/6862-cjas ◽

2013 ◽

Vol 58 (No. 7) ◽

pp. 321-327 ◽

Cited By ~ 7

Author(s):

X.P. An ◽

J.X. Hou ◽

H.B. Zhao ◽

L. Bai ◽

J.Y. Peng ◽

...

Keyword(s):

Dna Sequencing ◽

Milk Production ◽

Milk Yield ◽

Milk Fat ◽

Production Traits ◽

Milk Production Traits ◽

Dgat1 Gene ◽

Pcr Rflp ◽

Stat5a Gene ◽

T Locus

Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the DGAT1 gene, AJ237937:g.6798C>T and g.6852C>T in the STAT5A gene. In DGAT1 g.407_408insC locus, the frequencies of C– allele were 0.79–0.85, and frequencies of C+ allele were 0.21–0.15. At STAT5A g.6852C>T locus, frequencies of C allele were 0.70–0.72, and frequencies of T allele were 0.30–0.28. Compared with goats with DGAT1 C–C–, those with C–C+ genotype had greater milk fat (P < 0.05). The goats with STAT5A CT had greater milk yield than those with CC genotype (P < 0.05). The results showed that does with C–C–CT and C–C+CT yielded more milk than those with C–C–CC(P < 0.05). In addition, does with C–C+CT had the highest milk fat in comparison with other combination genotypes (P < 0.05).

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Effect of Breed and Some Non - Genetic Factors on Milk Production and Some Proportions of Its Chemical Components in Two Breeds of Local Sheep

Al-Qadisiyah Journal For Agriculture Sciences (QJAS) (P-ISSN 2077-5822 E-ISSN 2617-1479) ◽

10.33794/qjas.vol10.iss1.102 ◽

2020 ◽

Vol 10 (1) ◽

pp. 227-231

Author(s):

Rahman Hussein AL-Qasimi ◽

Shatha Mohammed Abbas ◽

Allawi L.D. AL-Khauzai

Keyword(s):

Milk Production ◽

Genetic Factors ◽

Chemical Components ◽

Lactation Period ◽

Production Traits ◽

Milk Production Traits ◽

Fat Percentage ◽

Awassi Sheep ◽

Total Milk ◽

Milk Components

The study was carried out on 19 ewes of local Awassi sheep and 12ewes local Arabi sheep in the Al-kafeel sheep station Karbala, to determine the effect of breed and some non-genetic factors such as (sex of the lamb, type of birth, age and weight of ewes at birth) on daily and total milk production and lactation period and some of milk components (fat, protein and lactose). The results showed that a significant effect (P <0.05) of the breed on milk production traits where Awassi sheep recorded the highest mean (0.91 kg , 101.63 kg , 104.86 day) compared to the Arabi sheep she was means (0.77 kg , 88.15 kg , 99.15 day) respectively. As well as in proportions of milk components with mean( 5.1 , 4.90 , 5.51) % respectively compared to the Arabi sheep (4.70 . 4.20 . 4.89) ewes with male lambs also exceeded superior ewes with female lambs in daily and total milk production and the lactation period the sex of the lamb did not affect the proportions of milk components the weight of the ewes had a significant effect (P <0.05) in milk production attributes with superior weight of ewes on lower ewes and did not affect the proportions of milk ingredients except for lactose. The type of birth and the age of the ewes did not have a significant effect in all the studied traits except for the superiority (P<0.05) of young ewes on age ewes in the fat percentage of milk.

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An association analysis between PRL genotype and milk production traits in Italian Mediterranean river buffalo

Journal of Dairy Research ◽

10.1017/s0022029917000693 ◽

2017 ◽

Vol 84 (4) ◽

pp. 430-433 ◽

Cited By ~ 6

Author(s):

Jun Li ◽

Aixin Liang ◽

Zipeng Li ◽

Chao Du ◽

Guohua Hua ◽

...

Keyword(s):

Milk Production ◽

Milk Yield ◽

Milk Fat ◽

Fat Content ◽

Phenotypic Variance ◽

River Buffalo ◽

Production Traits ◽

Milk Production Traits ◽

Exon 2 ◽

Mediterranean River

This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy–Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r2intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.

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