Agrobacterium tumefaciens-mediated transgenic plant production via direct shoot bud organogenesis from pre-plasmolyzed leaf explants of Catharanthus roseus

2011 ◽  
Vol 33 (5) ◽  
pp. 1053-1060 ◽  
Author(s):  
Priyanka Verma ◽  
Ajay Kumar Mathur
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plant ◽  
Catharanthus Roseus ◽  
Leaf Explants ◽  
Plant Production ◽  
Shoot Bud ◽  
Direct Shoot

scholarly journals Confirmation of “pre-plasmolysis mediated ex-osmosis hypothesis” to obtain shoot bud morphogenesis in Catharanthus roseus

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Vyoma Mistry ◽  
Abhishek Sharma ◽  
Ajay Kumar Mathur
Keyword(s):  
Genetic Engineering ◽  
Catharanthus Roseus ◽  
Cultured Cells ◽  
De Novo ◽  
Leaf Explants ◽  
Direct Regeneration ◽  
Engineering Process ◽  
Shoot Bud ◽  
Regeneration Response

AbstractThe antineoplastic herb, Catharanthus roseus is a classified high-value low-volume medicinal herb which is in global attention of scientific research for modulation of its monoterpenoid indole alkaloids (MIA) pathway through genetic engineering. These secondary metabolites are generally stored in specific types of structures/compartments due to their cytotoxic nature and designated roles in plant defense response. However, their presence can hinder the genetic engineering process used to develop transgenic plants through de novo morphogenesis and regeneration of plants from cultured cells/tissues and hence, it always remained a critical impediment in transgenic research in C. roseus. The pre-plasmolysis treatment of leaf explants can help to tackle the recalcitrant nature of leaf explant and can support the direct regeneration response by ex-osmosis that minimizes the concentration of alkaloids. Therefore, this study was performed to chase the effect of osmotic conditions on recalcitrant leaves of C. roseus engaged in vitro plant regeneration and hypothesis of alkaloids ex-osmosis is confirmed by HPLC analysis.

Direct shoot regeneration from leaf explants of Rosa damascena Mill.

2004 ◽  
Vol 40 (2) ◽  
pp. 192-195 ◽  
Author(s):  
Pratap Kumar Pati ◽  
Madhu Sharma ◽  
Anil Sood ◽  
P. S. Ahuja
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Rosa Damascena ◽  
Direct Shoot Regeneration ◽  
Direct Shoot

scholarly journals Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants

2010 ◽  
Vol 13 (4) ◽  
Author(s):  
Guadalupe Fabiola Arcos-Ortega ◽  
Rafael Antonio Chan-Kuuk ◽  
Wilma Aracely González-Kantún ◽  
Ramón Souza-Perera ◽  
Yumi Elena Nakazawa-Ueji ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Leaf Explants ◽  
Capsicum Chinense ◽  
Habanero Pepper
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