The impact of inducible promoters in transgenic plant production and crop improvement

Plant Gene ◽  
2021 ◽  
pp. 100300
Author(s):  
Saireet Misra ◽  
Markkandan Ganesan
Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 583
Author(s):  
Reda E. Abdelhameed ◽  
Nagwa I. Abu-Elsaad ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Rabab A. Metwally

Important gaps in knowledge remain regarding the potential of nanoparticles (NPs) for plants, particularly the existence of helpful microorganisms, for instance, arbuscular mycorrhizal (AM) fungi present in the soil. Hence, more profound studies are required to distinguish the impact of NPs on plant growth inoculated with AM fungi and their role in NP uptake to develop smart nanotechnology implementations in crop improvement. Zinc ferrite (ZnFe2O4) NPs are prepared via the citrate technique and defined by X-ray diffraction (XRD) as well as transmission electron microscopy for several physical properties. The analysis of the XRD pattern confirmed the creation of a nanocrystalline structure with a crystallite size equal to 25.4 nm. The effects of ZnFe2O4 NP on AM fungi, growth and pigment content as well as nutrient uptake of pea (Pisum sativum) plants were assessed. ZnFe2O4 NP application caused a slight decrease in root colonization. However, its application showed an augmentation of 74.36% and 91.89% in AM pea plant shoots and roots’ fresh weights, respectively, compared to the control. Moreover, the synthesized ZnFe2O4 NP uptake by plant roots and their contents were enhanced by AM fungi. These findings suggest the safe use of ZnFe2O4 NPs in nano-agricultural applications for plant development with AM fungi.


Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1675
Author(s):  
Hamada AbdElgawad ◽  
Walid Abuelsoud ◽  
Mahmoud M. Y. Madany ◽  
Samy Selim ◽  
Gaurav Zinta ◽  
...  

The use of actinomycetes for improving soil fertility and plant production is an attractive strategy for developing sustainable agricultural systems due to their effectiveness, eco-friendliness, and low production cost. Out of 17 species isolated from the soil rhizosphere of legume crops, 4 bioactive isolates were selected and their impact on 5 legumes: soybean, kidney bean, chickpea, lentil, and pea were evaluated. According to the morphological and molecular identification, these isolates belong to the genus Streptomyces. Here, we showed that these isolates increased soil nutrients and organic matter content and improved soil microbial populations. At the plant level, soil enrichment with actinomycetes increased photosynthetic reactions and eventually increased legume yield. Actinomycetes also increased nitrogen availability in soil and legume tissue and seeds, which induced the activity of key nitrogen metabolizing enzymes, e.g., glutamine synthetase, glutamate synthase, and nitrate reductase. In addition to increased nitrogen-containing amino acids levels, we also report high sugar, organic acids, and fatty acids as well as antioxidant phenolics, mineral, and vitamins levels in actinomycete treated legume seeds, which in turn improved their seed quality. Overall, this study shed the light on the impact of actinomycetes on enhancing the quality and productivity of legume crops by boosting the bioactive primary and secondary metabolites. Moreover, our findings emphasize the positive role of actinomycetes in improving the soil by enriching its microbial population. Therefore, our data reinforce the usage of actinomycetes as biofertilizers to provide sustainable food production and achieve biosafety.


2019 ◽  
Vol 14 (2) ◽  
pp. 492-505 ◽  
Author(s):  
Ian Lorenzo Quibod ◽  
Genelou Atieza-Grande ◽  
Eula Gems Oreiro ◽  
Denice Palmos ◽  
Marian Hanna Nguyen ◽  
...  

Abstract The impact of modern agriculture on the evolutionary trajectory of plant pathogens is a central question for crop sustainability. The Green Revolution replaced traditional rice landraces with high-yielding varieties, creating a uniform selection pressure that allows measuring the effect of such intervention. In this study, we analyzed a unique historical pathogen record to assess the impact of a major resistance gene, Xa4, in the population structure of Xanthomonas oryzae pv. oryzae (Xoo) collected in the Philippines in a span of 40 years. After the deployment of Xa4 in the early 1960s, the emergence of virulent pathogen groups was associated with the increasing adoption of rice varieties carrying Xa4, which reached 80% of the total planted area. Whole genomes analysis of a representative sample suggested six major pathogen groups with distinctive signatures of selection in genes related to secretion system, cell-wall degradation, lipopolysaccharide production, and detoxification of host defense components. Association genetics also suggested that each population might evolve different mechanisms to adapt to Xa4. Interestingly, we found evidence of strong selective sweep affecting several populations in the mid-1980s, suggesting a major bottleneck that coincides with the peak of Xa4 deployment in the archipelago. Our study highlights how modern agricultural practices facilitate the adaptation of pathogens to overcome the effects of standard crop improvement efforts.


Author(s):  
Abdullah B. Makhzoum ◽  
Pooja Sharma ◽  
Mark A. Bernards ◽  
Jocelyne Trémouillaux-Guiller

1996 ◽  
Vol 32 (4) ◽  
pp. 445-460 ◽  
Author(s):  
J. R. Witcombe ◽  
A. Joshi ◽  
K. D. Joshi ◽  
B. R. Sthapit

SUMMARYFarmer participatory approaches for the identification or breeding of improved crop cultivars can be usefully categorized into participatory varietal selection (PVS) and participatory plant breeding (PPB). Various PVS and PPB methods are reviewed. PVS is a more rapid and cost-effective way of identifying farmer-preferred cultivars if a suitable choice of cultivars exists. If this is impossible, then the more resource-consuming PPB is required. PPB can use, as parents, cultivars that were identified in successful PVS programmes. Compared with conventional plant breeding, PPB is more likely to produce farmer-acceptable products, particularly for marginal environments. The impact of farmer participatory research on biodiversity is considered. The long-term effect of PVS is to increase biodiversity, but where indigenous variability is high it can also reduce it. PPB has a greater effect on increasing biodiversity although its impact may be limited to smaller areas. PPB can be a dynamic form of in situ genetic conservation.


Author(s):  
John Igo ◽  
Charles E. Andraka

Dish Stirling power generation systems have been identified by DOE, Sandia National Laboratories, and Stirling Energy Systems (SES) as having the capability of delivering utility-scale renewable energy to the nation’s electrical grid. SES has proposed large plants, 20,000 units or more (0.5 GW rated power) in one place, in order to rapidly ramp up production automation. With the large capital investment needed in such a plant it becomes critical to optimize the system at the field level, as well as at the individual unit level. In this new software model, we provide a tool that predicts the annual and monthly energy performance of a field of dishes, in particular taking into account the impact of dish-to-dish shading on the energy and revenue streams. The Excel-based model goes beyond prior models in that it incorporates the true dish shape (flexible to accommodate many dish designs), multiple-row shading, and a revenue stream model that incorporates time-of-day and time-of-year pricing. This last feature is critical to understanding key shading tradeoffs on a financial basis. The model uses TMY or 15-minute meteorological data for the selected location. It can incorporate local ground slope across the plant, as well as stagger between the rows of dish systems. It also incorporates field-edge effects, which can be significant on smaller plants. It also incorporates factors for measured degraded performance due to shading. This tool provides one aspect of the decision process for fielding many systems, and must be combined with land costs, copper layout and costs, and O&M predictions (driving distance issues) in order to optimize the loss of power due to shading against the added expense of a larger spatial array. Considering only the energy and revenue stream, the model indicates that a rectangular, unstaggered field layout maximizes field performance. We also found that recognizing and accounting for true performance degradation due to shading significantly impacts plant production, compared with prior modeling attempts.


2006 ◽  
Vol 42 (2) ◽  
pp. 127-146 ◽  
Author(s):  
C. M. STIRLING ◽  
D. HARRIS ◽  
J. R. WITCOMBE

There is no one widely accepted method of managing international agricultural research and numerous different models exist. Here we review one in particular, referred to as the ‘institute without walls’, from the perspective of the UK Department for International Development's (DFID) Renewable Natural Resource (RNR) Research Strategy (1990–2006). We begin with a brief history of the RNR Research Strategy from 1990 to 2004. We then draw on nearly 15 years experience of managing one of the programmes within the RNR Research Strategy to assess critically the impact of externally and internally imposed organizational and management changes on the performance of the DFID Plant Sciences Programme (PSP). The current RNR Research Strategy (1995–2006), with its emphasis on demand-led research, has greatly increased the relevance and effectiveness of DFID's natural resources research. A comparison between the PSP in 2004 and the early 1990s inevitably concludes that the programme has been transformed: unlike in 1991, research is now firmly demand-driven, much is based in developing countries and farmers are benefiting from the research. Over time, the outputs of the long-term strategic research have been applied in practical plant breeding and participatory crop improvement programmes. Key to the success of the PSP has been the provision of continuous, long-term funding which has allowed projects time to develop and produce outputs of real value to end users. Alongside this, the ability of the PSP to build long-term, in-country partnerships has ensured the effective adoption of its research outputs. We conclude that the successes of the PSP have largely derived from (i) identification of research that is clearly demand driven, (ii) continuous long-term funding that has allowed research to move from the strategic to adaptive phase, (iii) continuity of management, and (iv) the flexibility to develop a wide range of partnerships, both in-country and overseas, based on their ability to deliver.


2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Naima Werfelli ◽  
Rayda Ben Ayed ◽  
Mejda Abassi ◽  
Zoubeir Béjaoui

The present work focused on the impact of crop irrigation by treated wastewater (TWW) on soil fertility, in germination, and growth of two species of cereals (T. turgidum and H. vulgare). This investigation was conducted at the germination stage (controlled condition) and in pots containing a soil irrigated with wastewater in comparison with controlled soil. Germination rate, vigor index, seedling growth, total fresh mass, chlorophyll content, proline, ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and catalase (CAT) activities were measured. Similar effects were shown on both species which emphasize the important role of antioxidant enzymes in the defense against oxidative stress induced by prolonged reuse of TWW. The disturbing effect of the reuse TWW on soil fertility, germination, and development of young plants (T. turgidum and H. vulgare) was linked to the presence of micropollutants in TWW. Data were analyzed by R language using a nonparametric statistical hypothesis test. These have caused the disorganization of many physiological mechanism targets, especially growth disorders observed under different abiotic stress conditions. In conclusion, high salt and heavy metal concentrations contained in the TWW are the major constraints related to the reuse of TWW. Hence, repetitive irrigation with this water can induce, at long term, soil contamination which can limit plant production and crop contamination.


2021 ◽  
Vol 57 (9) ◽  
pp. 6574-6583
Author(s):  
Muhammad Ayip Fathani, Erry Rimawan, Antonius Setyadi, Bambang Setiawan

In the industrialized world, increasing productivity and profitability is very important for companies as a benchmark of success in business processes. Some literature has defined the success factor of the successful application of the concept of TPM as a tool to improve the productivity performance of the company, therefore improving the productivity performance of the company becomes the main thing in improving long-term sustainable profitability for the company . In the manufacturing industry pulp and paper mills, the contribution of the highest production costs is in raw materials (Pulp and chemical raw materials), followed by energy and packaging. The energy sector occupies the top three in the cost contributor to variable costs, it is triggered because in the pulp paper industry sector, the consumption value for electrical energy and heat is very high. Therefore, success in eliminating eight major losses is a major success factor in improving the profitability of the company. To analyze the effects of elimination of eight major losses, reduction of production costs and increase in profitability can be solved by evaluating the influence of all indicators of eight major losses using PLS-SEM. The data used is operational data co-generation plant production of PT. XYZ in 2019. From the research conducted it is known that the variable Overall Plant Effectiveness (OPE) is significantly influenced by 2 (two) of the 3 (three) constituent indicators, namely Availability and Performance, both indicators affect: Productivity Improvement, Decreased Production Costs and Increased Profitability of the Company. The priority of improvement that must be done by considering the production cost performance data from the review of variable costs of energy is elimination: Loss Shutdown losses (A1), Loss Production adjustment loss (A2), Equipment failure (A3), Process failure ( A4), Normal Production (A5), and abnormal production (A6).


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