Improvement of short-term hypothermic preservation of microencapsulated hepatocytes

The feasibility of University of Wisconsin (UW) solution in short-term hypothermic preservation of porcine hepatocyte spheroids was investigated, because they have great potential in bioartificial liver (BAL) systems. Porcine hepatocyte spheroids preserved for 3 days expressed almost comparable levels of albumin secretion as those without preservation, during 8 subsequent days of recultivation in continuous rotational culture, whereas isolated single cells did not reorganize into spheroids and completely lost their function in recultivation. Although for 3-day–preserved spheroids, the albumin secretion was lowered immediately after recultivation (Days 0–2), it was completely restored to that of nonpreserved ones. The function was completely lost in recultivation for 7-day–preserved ones. These results demonstrate that reorganization into spheroids is effective in preventing the functional loss of porcine hepatocytes occurring in hypothermic preservation, and that spheroid formation should precede the preservation as long as spheroid culture is finally used in BAL systems. Also, porcine hepatocyte spheroids are shown to be satisfactory stored in UW solution up to 3 days without significant cellular or functional loss.

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SUL-109 Protects Hematopoietic Stem Cells from Apoptosis Induced by Short-Term Hypothermic Preservation and Maintains Their Engraftment Potential

Biology of Blood and Marrow Transplantation ◽

10.1016/j.bbmt.2019.12.770 ◽

2020 ◽

Vol 26 (4) ◽

pp. 634-642

Author(s):

Fatima S.F. Aerts-Kaya ◽

Trudi P. Visser ◽

Burcu Pervin ◽

Aynura Mammadova ◽

Özgür Özyüncü ◽

...

Keyword(s):

Stem Cells ◽

Hematopoietic Stem Cells ◽

Short Term ◽

Hematopoietic Stem ◽

Hypothermic Preservation

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Short-term hypothermic preservation of human testicular tissue: the effect of storage medium and storage period

Fertility and Sterility ◽

10.1016/j.fertnstert.2016.01.018 ◽

2016 ◽

Vol 105 (5) ◽

pp. 1162-1169.e5 ◽

Cited By ~ 4

Author(s):

Katrien Faes ◽

Ellen Goossens

Keyword(s):

Storage Period ◽

Testicular Tissue ◽

Storage Medium ◽

Short Term ◽

Hypothermic Preservation ◽

And Storage

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139 Low temperature cell pausing: A short-term hypothermic preservation method for cells

Cryobiology ◽

10.1016/j.cryobiol.2013.09.145 ◽

2013 ◽

Vol 67 (3) ◽

pp. 437-438

Author(s):

Nathalie J. Robinson ◽

Andrew Picken ◽

Christopher J. Hewitt

Keyword(s):

Low Temperature ◽

Short Term ◽

Hypothermic Preservation ◽

Preservation Method

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Conceptual short-term memory (CSTM) supports core claims of Christiansen and Chater

Behavioral and Brain Sciences ◽

10.1017/s0140525x15000928 ◽

2016 ◽

Vol 39 ◽

Author(s):

Mary C. Potter

Keyword(s):

Working Memory ◽

Rapid Serial Visual Presentation ◽

Language Comprehension ◽

Short Term Memory ◽

Visual Presentation ◽

Long Term Memory ◽

Short Term ◽

Term Memory ◽

Use Of Knowledge

AbstractRapid serial visual presentation (RSVP) of words or pictured scenes provides evidence for a large-capacity conceptual short-term memory (CSTM) that momentarily provides rich associated material from long-term memory, permitting rapid chunking (Potter 1993; 2009; 2012). In perception of scenes as well as language comprehension, we make use of knowledge that briefly exceeds the supposed limits of working memory.

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Ultrastructural Changes of Canine Kidneys During 24-Hour Perfusion on a LI-400 Preservation System

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065626 ◽

1971 ◽

Vol 29 ◽

pp. 316-317

Author(s):

M. O. Magnusson ◽

D. G. Osborne ◽

T. Shimoji ◽

W. S. Kiser ◽

W. A. Hawk

Keyword(s):

Electron Microscopy ◽

Electrolyte Solution ◽

Ultrastructural Changes ◽

Midline Incision ◽

Short Term ◽

Pentobarbital Anesthesia ◽

Pulsatile Perfusion

Short term experimental and clinical preservation of kidneys is presently best accomplished by hypothermic continuous pulsatile perfusion with cryoprecipitated and millipore filtered plasma. This study was undertaken to observe ultrastructural changes occurring during 24-hour preservation using the above mentioned method.A kidney was removed through a midline incision from healthy mongrel dogs under pentobarbital anesthesia. The kidneys were flushed immediately after removal with chilled electrolyte solution and placed on a LI-400 preservation system and perfused at 8-10°C. Serial kidney biopsies were obtained at 0-½-1-2-4-8-16 and 24 hours of preservation. All biopsies were prepared for electron microscopy. At the end of the preservation period the kidneys were autografted.

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