Optimization for allitol production from d-glucose by using immobilized glucose isomerase and recombinant E. coli expressing d-psicose-3-epimerase, ribitol dehydrogenase and formate dehydrogenase

2020 ◽  
Vol 42 (11) ◽  
pp. 2135-2145
Author(s):  
Xin Wen ◽  
Huibin Lin ◽  
Yilin Ren ◽  
Can Li ◽  
Chengjia Zhang ◽  
...  
Keyword(s):  
Formate Dehydrogenase ◽  
Glucose Isomerase ◽  
E Coli ◽  
Immobilized Glucose Isomerase ◽  
Ribitol Dehydrogenase

scholarly journals Facile synthesis of bioactive Allitol from D-psicose by coexpression of ribitol dehydrogenase and formate dehydrogenase in Escherichia Coli

2018 ◽  
Vol 4 ◽  
Author(s):  
Hinawi A.M. Hassanin ◽  
Mohammed A.A. Eassa ◽  
Bo Jiang
Keyword(s):  
Escherichia Coli ◽  
Substrate Concentration ◽  
Formate Dehydrogenase ◽  
Maximum Yield ◽  
Optimum Conditions ◽  
Facile Synthesis ◽  
Nadh Regeneration ◽  
E Coli ◽  
Ribitol Dehydrogenase

Coexpression of formate dehydrogenase (FDH) and ribitol dehydrogenase (RDH) in Escherichia coli was used for the synthesis of Allitol from D-psicose. FDH was coexpressed with RDH for continuous NADH regeneration. The results revealed that the optimum conditions for allitol production occurred at pH 7.0 and 30 °C. Allitol reached the maximum yield of 19.2 mg at 2.0% substrate concentration after 48 hours. Using D-psicose as a substrate, allitol was successfully produced using an engineered E. coli coexpressed with RDH and FDH.

scholarly journals Structural genes for nitrate-inducible formate dehydrogenase in Escherichia coli K-12.

Genetics ◽  
1990 ◽  
Vol 125 (4) ◽  
pp. 691-702 ◽  
Author(s):  
B L Berg ◽  
V Stewart
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Formate Dehydrogenase ◽  
Recombinant Dna ◽  
Structural Genes ◽  
Respiratory Pathway ◽  
E Coli ◽  
Formate Oxidation ◽  
Operon Expression ◽  
K 12

Abstract Formate oxidation coupled to nitrate reduction constitutes a major anaerobic respiratory pathway in Escherichia coli. This respiratory chain consists of formate dehydrogenase-N, quinone, and nitrate reductase. We have isolated a recombinant DNA clone that likely contains the structural genes, fdnGHI, for the three subunits of formate dehydrogenase-N. The fdnGHI clone produced proteins of 110, 32 and 20 kDa which correspond to the subunit sizes of purified formate dehydrogenase-N. Our analysis indicates that fdnGHI is organized as an operon. We mapped the fdn operon to 32 min on the E. coli genetic map, close to the genes for cryptic nitrate reductase (encoded by the narZ operon). Expression of phi(fdnG-lacZ) operon fusions was induced by anaerobiosis and nitrate. This induction required fnr+ and narL+, two regulatory genes whose products are also required for the anaerobic, nitrate-inducible activation of the nitrate reductase structural gene operon, narGHJI. We conclude that regulation of fdnGHI and narGHJI expression is mediated through common pathways.

Co-expression of l-arabinose isomerase and d-glucose isomerase in E. coli and development of an efficient process producing simultaneously d-tagatose and d-fructose

2007 ◽  
Vol 40 (6) ◽  
pp. 1531-1537 ◽  
Author(s):  
Moez Rhimi ◽  
Ezzedine Ben Messaoud ◽  
Mohamed Ali Borgi ◽  
Khalifa Ben khadra ◽  
Samir Bejar
Keyword(s):  
Glucose Isomerase ◽  
E Coli ◽  
Efficient Process ◽  
Arabinose Isomerase
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