Molecular characterization of ginseng farnesyl diphosphate synthase gene and its up-regulation by methyl jasmonate

2010 ◽  
Vol 54 (1) ◽  
pp. 47-53 ◽  
Author(s):  
O. T. Kim ◽  
K. H. Bang ◽  
S. J. Jung ◽  
Y. C. Kim ◽  
D. Y. Hyun ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Molecular Characterization ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase

Methyl jasmonate-induced enhancement of expression activity of Am-FaPS-1, a putative farnesyl diphosphate synthase gene from Aquilaria microcarpa

2010 ◽  
Vol 65 (1) ◽  
pp. 194-197 ◽  
Author(s):  
Yoshihide Kenmotsu ◽  
Shinjiro Ogita ◽  
Yasuo Katoh ◽  
Yoshimi Yamamura ◽  
Yasumasa Takao ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Expression Activity

Cloning, expression and characterization of a dipteran farnesyl diphosphate synthase

2007 ◽  
Vol 37 (11) ◽  
pp. 1198-1206 ◽  
Author(s):  
Stephanie E. Sen ◽  
Corey Trobaugh ◽  
Catherine Béliveau ◽  
Thenesha Richard ◽  
Michel Cusson
Keyword(s):  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase

scholarly journals Characterization of Potential Drug Targets Farnesyl Diphosphate Synthase and Geranylgeranyl Diphosphate Synthase in Schistosoma mansoni

2013 ◽  
Vol 57 (12) ◽  
pp. 5969-5976 ◽  
Author(s):  
Peter D. Ziniel ◽  
Janish Desai ◽  
Cynthia L. Cass ◽  
Craig Gatto ◽  
Eric Oldfield ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Drug Targets ◽  
Protein Characterization ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Potential Drug ◽  
Geranylgeranyl Diphosphate Synthase ◽  
Geranylgeranyl Diphosphate ◽  
Potential Drug Targets

ABSTRACTSchistosomiasis affects over 200 million people worldwide, with over 200,000 deaths annually. Currently, praziquantel is the only drug available against schistosomiasis. We report here thatSchistosoma mansonifarnesyl diphosphate synthase (SmFPPS) and geranylgeranyl diphosphate synthase (SmGGPPS) are potential drug targets for the treatment of schistosomiasis. We expressed active, recombinantSmFPPS andSmGGPPS for subsequent kinetic characterization and testing against a variety of bisphosphonate inhibitors. RecombinantSmFPPS was found to be a soluble 44.2-kDa protein, whileSmGGPPS was a soluble 38.3-kDa protein. Characterization of the substrate utilization of the two enzymes indicates that they have overlapping substrate specificities. AgainstSmFPPS, several bisphosphonates had 50% inhibitory concentrations (IC50s) in the low micromolar to nanomolar range; these inhibitors had significantly less activity againstSmGGPPS. Several lipophilic bisphosphonates were active againstex vivoadult worms, with worm death occurring over 4 to 6 days. These results indicate that FPPS and GGPPS could be of interest in the context of the emerging resistance to praziquantel in schistosomiasis therapy.

scholarly journals Cloning and Molecular Characterization of CfMYBs Associated with the Regulation of Methyl Jasmonate Biosynthesis in Cymbidium faberi

2020 ◽  
Vol 89 (5) ◽  
pp. 593-601
Author(s):  
Yanqin Xu ◽  
Junjiang Zhou ◽  
Song Lu ◽  
Songtai Wang ◽  
Yin Zhou
Keyword(s):  
Methyl Jasmonate ◽  
Molecular Characterization
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