Silica-gold nanoshells as potential intraoperative molecular probes for HER2-overexpression in ex vivo breast tissue using near-infrared reflectance confocal microscopy

2009 ◽  
Vol 120 (3) ◽  
pp. 547-555 ◽  
Author(s):  
Lissett R. Bickford ◽  
Germaine Agollah ◽  
Rebekah Drezek ◽  
Tse-Kuan Yu
Keyword(s):  
Confocal Microscopy ◽  
Breast Tissue ◽  
Near Infrared ◽  
Ex Vivo ◽  
Molecular Probes ◽  
Gold Nanoshells ◽  
Reflectance Confocal Microscopy ◽  
Her2 Overexpression ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance

Novel Virtual Biopsy of the Kidney With Near Infrared, Reflectance Confocal Microscopy: A Pilot Study In Vivo and Ex Vivo

2006 ◽  
Vol 175 (1) ◽  
pp. 327-336 ◽  
Author(s):  
Vanessa Campo-Ruiz ◽  
Gregory Y. Lauwers ◽  
R. Rox Anderson ◽  
Emilio Delgado-Baeza ◽  
Salvador González
Keyword(s):  
Pilot Study ◽  
Confocal Microscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Reflectance Confocal Microscopy ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance

scholarly journals In vivo and ex vivo virtual biopsy of the liver with near-infrared, reflectance confocal microscopy

2004 ◽  
Vol 18 (2) ◽  
pp. 290-300 ◽  
Author(s):  
Vanessa Campo-Ruiz ◽  
Gregory Y Lauwers ◽  
R Rox Anderson ◽  
Emilio Delgado-Baeza ◽  
Salvador González
Keyword(s):  
Confocal Microscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Reflectance Confocal Microscopy ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance

Virtual biopsy of the joint tissues using near-infrared, reflectance confocal microscopy. A pilot study

2006 ◽  
Vol 69 (10) ◽  
pp. 794-798 ◽  
Author(s):  
Vanessa Campo-Ruiz ◽  
Dinesh Patel ◽  
R. Rox Anderson ◽  
Emilio Delgado-Baeza ◽  
Salvador González
Keyword(s):  
Pilot Study ◽  
Confocal Microscopy ◽  
Near Infrared ◽  
Reflectance Confocal Microscopy ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance

Novel Virtual Biopsy of the Kidney With Near Infrared, Reflectance Confocal Microscopy

2006 ◽  
Vol 175 (1) ◽  
pp. 327???336
Author(s):  
Vanessa Campo-Ruiz ◽  
Gregory Y. Lauwers ◽  
R. Rox Anderson ◽  
Emilio Delgado-Baeza ◽  
Salvador Gonz??lez
Keyword(s):  
Confocal Microscopy ◽  
Near Infrared ◽  
Reflectance Confocal Microscopy ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance

scholarly journals Rapid Stereomicroscopic Imaging of HER2 Overexpression inEx VivoBreast Tissue Using Topically Applied Silica-Based Gold Nanoshells

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Lissett R. Bickford ◽  
Robert J. Langsner ◽  
Joseph Chang ◽  
Laura C. Kennedy ◽  
Germaine D. Agollah ◽  
...  
Keyword(s):  
Normal Tissue ◽  
Breast Tissue ◽  
Ex Vivo ◽  
Breast Conservation ◽  
Receptor Expression ◽  
Gold Nanoshells ◽  
Her2 Overexpression ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Near Infrared Reflectance

Tumor margin detection for patients undergoing breast conservation surgery primarily occurs postoperatively. Previously, we demonstrated that gold nanoshells rapidly enhance contrast of HER2 overexpression inex vivotissue sections. Our ultimate objective, however, is to discern HER2 overexpressing tissue from normal tissue in whole, nonsectioned, specimens to facilitate rapid diagnoses. Here, we use targeted nanoshells to quickly and effectively visualize HER2 receptor expression in intactex vivohuman breast tissue specimens. Punch biopsies of human breast tissue were analyzed after a brief 5-minute incubation with and without HER2-targeted silica-gold nanoshells using two-photon microscopy and stereomicroscopy. Labeling was subsequently verified using reflectance confocal microscopy, darkfield hyperspectral imaging, and immunohistochemistry to confirm levels of HER2 expression. Our results suggest that anti-HER2 nanoshells used in tandem with a near-infrared reflectance confocal microscope and a standard stereomicroscope may potentially be used to discern HER2-overexpressing cancerous tissue from normal tissue in near real time and offer a rapid supplement to current diagnostic techniques.

scholarly journals Visible near infrared reflectance molecular chemical imaging of human ex vivo carcinomas and murine in vivo carcinomas

2020 ◽  
Vol 25 (02) ◽  
pp. 1
Author(s):  
Shona Stewart ◽  
Marlena Darr ◽  
Heather Gomer ◽  
Aaron Smith ◽  
Arash Samiei ◽  
...  
Keyword(s):  
Near Infrared ◽  
Ex Vivo ◽  
Chemical Imaging ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance
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