Clinical therapy with either interferon alpha or interferon gamma is associated with a febrile response. However, the effects of hyperthermia on the response to these interferons have not been elucidated fully. in this study, a cell line derived from a laryngeal squamous cell carcinoma (JHU-011-SCC-L-P) was grown in the presence of recombinant human interferon alpha (rHulFNα) or recombinant human Interferon gamma (rHulFNγ) and incubated at either 37° or 39° C, and cell growth rates were measured. Cells incubated with rHulFNα demonstrated no difference in growth rates from control cells. Cells treated with rHulFNγ showed significant inhibition of growth at both temperatures, and the ratio of decreased growth at 39° C was significantly greater than for the rHulFNα and control groups. This hyperthermic effect did not depend on the continued exposure to rHulFNγ, and the effect appeared to depend on the duration of hyperthermia instead of the time sequence of hyperthermic exposure. Moreover, initial treatment at 39° C for 24 hours was ineffective in producing the hyperthermic response produced with continuous hyperthermic exposure. These findings would indicate that the effect of rHulFNγ on this laryngeal squamous cell carcinoma cell line is enhanced significantly at 39° C compared with 37°C. There appears to be no similar hyperthermia-augmented antiproliferative effect of rHulFNα-treated cells.
EFFECT OF EUCALYPTUS OIL AND 5-FLUOROURACIL ON HEAD AND NECK SQUAMOUS CELL CARCINOMA CELL LINE” IN-VITRO STUDY
