Comparative study of decellularization techniques to obtain natural extracellular matrix scaffolds of human peripheral-nerve allografts

2021 ◽  
Author(s):  
Paula Hansen Suss ◽  
Victoria Stadler Tasca Ribeiro ◽  
Carlos Eduardo Motooka ◽  
Letícia Corso de Melo ◽  
Felipe Francisco Tuon
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Comparative Study ◽  
Extracellular Matrix Scaffolds

scholarly journals Augmentation Cystoplasty and Extracellular Matrix Scaffolds: An Ex Vivo Comparative Study with Autogenous Detubularised Ileum

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e20323 ◽  
Author(s):  
Niall F. Davis ◽  
Rory Mooney ◽  
Anthony Callanan ◽  
Hugh D. Flood ◽  
Tim M. McGloughlin
Keyword(s):  
Extracellular Matrix ◽  
Comparative Study ◽  
Ex Vivo ◽  
Augmentation Cystoplasty ◽  
Extracellular Matrix Scaffolds

Closure of Fetoscopic Access Sites with Amniotic Extracellular Matrix Scaffolds in an In Vivo Model

2006 ◽  
Vol 66 (S 01) ◽  
Author(s):  
N Ochsenbein-Kölble ◽  
J Jani ◽  
G Verbist ◽  
L Lewi ◽  
K Marquardt ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vivo Model ◽  
Extracellular Matrix Scaffolds

Experimental Tracheal Patching Using Extracellular Matrix Scaffolds

2009 ◽  
Vol 87 (4) ◽  
pp. 1321-1322 ◽  
Author(s):  
Johanna Schanz ◽  
Martina Hampel ◽  
Heike Mertsching ◽  
Thorsten Walles
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Scaffolds

scholarly journals The Physicochemical Properties of Decellularized Extracellular Matrix-Coated 3D Printed Poly(ε-caprolactone) Nerve Conduits for Promoting Schwann Cells Proliferation and Differentiation

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1665 ◽  
Author(s):  
Chung-Chia Chen ◽  
Joyce Yu ◽  
Hooi-Yee Ng ◽  
Alvin Lee ◽  
Chien-Chang Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Schwann Cells ◽  
Alternative Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Class ◽  
Nerve Grafting ◽  
Decellularized Extracellular Matrix ◽  
Nerve Conduits ◽  
3D Printed

Although autologous nerve grafting remains the gold standard treatment for peripheral nerve injuries, alternative methods such as development of nerve guidance conduits have since emerged and evolved to counter the many disadvantages of nerve grafting. However, the efficacy and viability of current nerve conduits remain unclear in clinical trials. Here, we focused on a novel decellularized extracellular matrix (dECM) and polydopamine (PDA)-coated 3D-printed poly(ε-caprolactone) (PCL)-based conduits, whereby the PDA surface modification acts as an attachment platform for further dECM attachment. We demonstrated that dECM/PDA-coated PCL conduits possessed higher mechanical properties when compared to human or animal nerves. Such modifications were proved to affect cell behaviors. Cellular behaviors and neuronal differentiation of Schwann cells were assessed to determine for the efficacies of the conduits. There were some cell-specific neuronal markers, such as Nestin, neuron-specific class III beta-tubulin (TUJ-1), and microtubule-associated protein 2 (MAP2) analyzed by enzyme-linked immunosorbent assay, and Nestin expressions were found to be 0.65-fold up-regulated, while TUJ1 expressions were 2.3-fold up-regulated and MAP2 expressions were 2.5-fold up-regulated when compared to Ctl. The methodology of PDA coating employed in this study can be used as a simple model to immobilize dECM onto PCL conduits, and the results showed that dECM/PDA-coated PCL conduits can as a practical and clinically viable tool for promoting regenerative outcomes in larger peripheral nerve defects.

A Comparative Study of the Toxicity of n-Pentane, n-Hexane, and n-Heptane to the Peripheral Nerve of the Rat

1981 ◽  
Vol 18 (12) ◽  
pp. 1395-1402 ◽  
Author(s):  
Y. Takeuchi ◽  
Y. Ono ◽  
N. Hisanaga ◽  
J. Kitoh ◽  
Y. Sugiura
Keyword(s):  
Peripheral Nerve ◽  
Comparative Study
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