Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity

2015 ◽  
Vol 142 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Qian Li ◽  
Rong-Jun Guo ◽  
Shi-Dong Li ◽  
Shi-Fang Li ◽  
Hong-Qing Wang
Keyword(s):  
Real Time ◽  
Disease Severity ◽  
Real Time Pcr ◽  
Crown Gall ◽  
Pcr Assay ◽  
Crown Gall Disease

Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

2013 ◽  
Vol 27 (4) ◽  
pp. 328-332 ◽  
Author(s):  
Stellan Håkansson ◽  
Karin Källén ◽  
Maria Bullarbo ◽  
Per-Åke Holmgren ◽  
Katarina Bremme ◽  
...  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Group B

scholarly journals Determination of Sex Ratio In Bovine Semen Using SYBR Green Real-Time PCR

2022 ◽  
Author(s):  
Vemula Harshini ◽  
S.M.K. Karthickeyan ◽  
K.G P. Kumarasamy ◽  
Tirumurugaan ◽  
C. Jeevan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Pcr Assay ◽  
Mean Values ◽  
Bovine Semen ◽  
Routine Basis ◽  
Repeatability And Reproducibility ◽  
The Mean

Abstract A SYBR green real-time PCR assay was developed to find out the sex skewness in bovine sex-sorted semen samples. The qPCR assay of PLP and SRY genes revealed the mean values of X- and Y-bearing spermatozoa as 50.24 ± 0.65 and 49.75 ± 0.62 per cent in unsorted, and 91.80 ± 0.79 and 8.20 ± 0.73 per cent in X-enriched semen samples respectively.. The amplification efficiencies of the PLP and SRY primers were 99.25 and 98.03 per cent respectively. The method was validated by a series of repeatability and reproducibility assays which revealed low co-efficients of variations as 2.19 and 3.12 per cent respectively Thus becoming a reliable and inexpensive tool to evaluate the sorted semen on routine basis and validation of other sperm sexing technologies.

scholarly journals A Highly Sensitive Quantitative Real-Time PCR Assay for Determination of Mutant JAK2 Exon 12 Allele Burden

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33100 ◽  
Author(s):  
Lasse Kjær ◽  
Maj Westman ◽  
Caroline Hasselbalch Riley ◽  
Estrid Høgdall ◽  
Ole Weis Bjerrum ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Allele Burden ◽  
Highly Sensitive

scholarly journals Application of Real-Time PCR for Determination of Antiviral Drug Susceptibility of Herpes Simplex Virus

2002 ◽  
Vol 46 (9) ◽  
pp. 2943-2947 ◽  
Author(s):  
Růžena Stránská ◽  
Anton M. van Loon ◽  
Merjo Polman ◽  
Rob Schuurman
Keyword(s):  
Herpes Simplex ◽  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Drug ◽  
Drug Susceptibility ◽  
Pcr Assay ◽  
Simplex Virus ◽  
Antiviral Drug Susceptibility ◽  
Hsv 1

ABSTRACT A quantitative real-time PCR (TaqMan) assay was developed for determination of antiviral drug susceptibility of herpes simplex virus (HSV). After short-time culture of the virus, the antiviral drug susceptibility of HSV isolates for acyclovir (ACV) was determined by measuring the reduction of the HSV type 1 (HSV-1) DNA levels in culture supernatants using real-time PCR. The 50% inhibitory concentration was reported as the concentration of antiviral drug that reduced the number of HSV-1 DNA copies by 50%. A total of 15 well-characterized ACV-sensitive or -resistant strains and clinical isolates were used for assay evaluation. The new assay with real-time PCR readout permitted rapid (3 days), objective, and reproducible determination of HSV-1 drug susceptibilities with no need for stringent control of initial multiplicity of infection. Furthermore, the real-time PCR assay results showed good correlation (r = 0.86) with those for the plaque reduction assay. In conclusion, the real-time PCR assay described here is a suitable quantitative method for determination of antiviral susceptibility of HSV-1, amenable for use in the routine diagnostic virology laboratory.

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