Assessment of Genetic Diversity in Upland Cotton (Gossypium hirsutum L.) Breeding Lines by using Amplified Fragment Length Polymorphism (AFLP) Markers and Morphological Characteristics

2005 ◽  
Vol 52 (8) ◽  
pp. 989-997 ◽  
Author(s):  
M. K. Rana ◽  
V. P. Singh ◽  
K. V. Bhat
Keyword(s):  
Genetic Diversity ◽  
Gossypium Hirsutum ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Upland Cotton ◽  
Fragment Length Polymorphism ◽  
Morphological Characteristics ◽  
Aflp Markers ◽  
Gossypium Hirsutum L ◽  
Breeding Lines

Assessment of Genetic Diversity in 31 Species of Mangroves and their Associates through RAPD and AFLP Markers

2006 ◽  
Vol 61 (5-6) ◽  
pp. 413-420 ◽  
Author(s):  
Arup Kumar Mukherjee ◽  
Laxmikanta Acharya ◽  
Pratap Chandra Panda ◽  
Trilochan Mohapatra
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Aflp Markers ◽  
The Family ◽  
Associated Species ◽  
High Degree

Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers were used to assess the genetic diversity in 31 species of mangroves and mangrove associates. Four AFLP primer combinations resulted in the amplification of 840 bands with an average of 210 bands per primer combination and 11 RAPD primers yielded 319 bands with an average of 29 bands per primer. The percentage of polymorphism detected was too high indicating the high degree of genetic variability in mangrove taxa both at inter- and intra-generic levels. In the dendrogram, species belonging to a particular family/ genus, taxa inhabiting similar habitats or having similar adaptations tended to be together. There were exceptions too; as many unrelated species of mangroves form clusters. The intrafamilial classification and inter-relationships of genera in the family Rhizophoraceae could be confirmed by molecular analysis. Both the markers RAPD and AFLP were found equally informative and useful for a better understanding of the genetic variability and genome relationships among mangroves and their associated species.

Modified AFLP technique for fingerprinting of Anemone coronaria

2005 ◽  
Vol 3 (1) ◽  
pp. 73-75
Author(s):  
J. Fang ◽  
T. Paz
Keyword(s):  
Genetic Diversity ◽  
Restriction Enzyme ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Aflp Markers ◽  
The One ◽  
Cultivar Differentiation ◽  
Very High ◽  
Aflp Technique

We successfully generated clear amplified fragment length polymorphism (AFLP) profiles of Anemone coronaria using only a single restriction enzyme (EcoR I). Four primer combinations generated 571 bands in 37 anemone accessions with an average 142.75 bands per primer combination. Polymorphism among the 37 samples was very high (98.15%). This high polymorphism showed that the one-enzyme AFLP method is effective for the assessment of genetic diversity as well as cultivar differentiation of anemone. We observed 26 bands which differentiated between fingerprints derived from leaf and corm DNA of eight plant individuals. However, the low percentage of such bands showed that anemone corms can also be used to evaluate anemone germplasm using AFLP markers.

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