Plasticity responses of an invasive macrophyte species to inorganic carbon availability and to the interaction with a native species

The presence of different carbonic anhydrase (EC 4.2.1.1) activities has been investigated in the intertidal macroalgae Gracilaria sp. and Enteromorpha intestinalis (L.) Nees by using fractionation techniques. Activities, measured potentiometrically, were recorded for all fractions in both species, including those containing proteins associated with chloroplast membranes. In Gracilaria sp., most of the total activity was present in the soluble fraction, while similar activities were obtained for all fractions in E. intestinalis. By using inhibitors with a different capacity to enter the cell (acetazolamide and 6-ethoxyzolamide, inhibitors of external and total activity, respectively), a surface-accessible location was indicated for a high proportion of the soluble activity obtained in Gracilaria sp. In E. intestinalis, the inhibitor assays showed a substantial dependence of photosynthesis on intracellular activity. The short-term regulation of the extracellular activity in response to inorganic carbon availability was also examined in both macroalgae. Rapid repression (after 2 h) of the activity was recorded when Gracilaria sp. was transferred from limited to replete carbon conditions, while a fairly constant activity was recorded for E. intestinalis. In contrast, an increase of external activity was obtained for both macroalgae after being transferred to carbon-limited conditions, this response being more pronounced in E. intestinalis. Our results suggest the occurrence of a species-specific carbonic anhydrase system.

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Acclimation of photosynthetic pigments and photosynthesis of the cyanobacterium Nostoc sp. strain UAM206 to combined fluctuations of irradiance, pH, and inorganic carbon availability

Journal of Plant Physiology ◽

10.1078/0176-1617-00555 ◽

2001 ◽

Vol 158 (11) ◽

pp. 1455-1461 ◽

Cited By ~ 30

Author(s):

César Poza-Carrión ◽

Eduardo Fernández-Valiente ◽

Francisca Fernández Piñas ◽

Francisco Leganés

Keyword(s):

Photosynthetic Pigments ◽

Inorganic Carbon ◽

Carbon Availability

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Growth and photosynthetic acclimation by Ranunculus aquatilis L. in response to inorganic carbon availability

New Phytologist ◽

10.1111/j.1469-8137.1993.tb03919.x ◽

1993 ◽

Vol 125 (4) ◽

pp. 707-715 ◽

Cited By ~ 24

Author(s):

TOM VINDAEK MADSEN

Keyword(s):

Inorganic Carbon ◽

Photosynthetic Acclimation ◽

Carbon Availability

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Growth limitation of three Arctic sea ice algal species: effects of salinity, pH, and inorganic carbon availability

Polar Biology ◽

10.1007/s00300-011-0976-3 ◽

2011 ◽

Vol 34 (8) ◽

pp. 1157-1165 ◽

Cited By ~ 19

Author(s):

Dorte Haubjerg Søgaard ◽

Per Juel Hansen ◽

Søren Rysgaard ◽

Ronnie Nøhr Glud

Keyword(s):

Sea Ice ◽

Inorganic Carbon ◽

Algal Species ◽

Arctic Sea Ice ◽

Growth Limitation ◽

Carbon Availability ◽

Species Effects ◽

Arctic Sea

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Vanishing coccolith vital effects with alleviated CO<sub>2</sub> limitation

Biogeosciences Discussions ◽

10.5194/bgd-12-15835-2015 ◽

2015 ◽

Vol 12 (18) ◽

pp. 15835-15866 ◽

Cited By ~ 1

Author(s):

M. Hermoso ◽

I. Z. X. Chan ◽

H. L. O. McClelland ◽

A. M. C. Heureux ◽

R. E. M. Rickaby

Keyword(s):

Inorganic Carbon ◽

Dissolved Inorganic Carbon ◽

Calcareous Nannofossils ◽

Carbon And Oxygen Isotopes ◽

Carbon Availability ◽

Vital Effect ◽

Vital Effects ◽

Multiple Species ◽

Atmospheric Co2 Concentrations

Abstract. By recreating a range of geologically relevant concentrations of dissolved inorganic carbon (DIC) in the laboratory, we demonstrate that the magnitude of the vital effects in both carbon and oxygen isotopes of coccolith calcite of multiple species relates to ambient DIC concentration. Under high DIC levels, all the examined coccoliths lacked any offset from inorganic calcite, whereas in low (present-day) DIC concentrations, these vital effects and interspecies differences become substantial. These laboratory observations support the recent hypothesis from field observations that the appearance of interspecific vital effect in coccolithophores coincides with the long-term Neogene decline of atmospheric CO2 concentrations. The present study brings further valuable constraints on coccolith isotopic compositions by demonstrating the threshold for the absence of vital effects under high DIC regimes. From a mechanistic viewpoint, we show that the vital effect is determined by physiology; growth rate, cell size and relative rates of photosynthesis and calcification, and a modulation of these parameters with ambient carbon availability. This study provides palaeoceanographers with a biogeochemical framework that can be utilised to further develop the use of calcareous nannofossils in palaeoceanography to derive sea surface temperature and pCO2 levels.

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Functional macrophyte trait variation as a response to the source of inorganic carbon acquisition

PeerJ ◽

10.7717/peerj.12584 ◽

2021 ◽

Vol 9 ◽

pp. e12584

Author(s):

Rafał Chmara ◽

Eugeniusz Pronin ◽

Józef Szmeja

Keyword(s):

Leaf Area ◽

Functional Traits ◽

Environmental Variables ◽

Inorganic Carbon ◽

Aquatic Macrophyte ◽

Secchi Depth ◽

Leaf Traits ◽

Wide Range ◽

Macrophyte Species ◽

Inorganic Carbon Acquisition

Background This study aims to compare variation in a range of aquatic macrophyte species leaf traits into three carbon acquisition groups: HCO3−, free CO2 and atmospheric CO2. Methods The leaf functional traits were measured for 30 species from 30 softwater lakes. Macrophyte species were classified into (1) free CO2, (2) atmospheric CO2 and (3) bicarbonate HCO3− groups. In each lake we collected water samples and measured eight environmental variables: depth, Secchi depth, photosynthetically active radiation (PAR), pH of water, conductivity, calcium concentration, total nitrogen and total phosphorus. In this study we applied the RLQ analysis to investigate the relationships between species functional traits (Q) and their relationship with environmental variables (R) constrained by species abundance (L). Results The results showed that: (1) Aquatic macrophytes exhibited high leaf trait variations as a response to different inorganic carbon acquisition; (2) Traits of leaves refer to the acquisition of carbon for photosynthesis and serve to maximise this process; (3) In the wide softwater habitat, macrophyte species exhibited an extreme range of leaf economic spectrum (leaf area, leaf dry weight and specific leaf area) and wide range of shape trait expressed as circularity; (4) Macrophyte leaf traits are the result of adaptation to carbon acquisition in ambient environment.

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First record of Eurasian Water-milfoil, Myriophyllum spicatum, for the Saint John River, New Brunswick

The Canadian Field-Naturalist ◽

10.22621/cfn.v132i3.1943 ◽

2019 ◽

Vol 132 (3) ◽

pp. 231-237 ◽

Cited By ~ 1

Author(s):

Meghann Bruce ◽

Tommi Linnansaari ◽

R. Allen Curry

Keyword(s):

New Brunswick ◽

Native Species ◽

Myriophyllum Spicatum ◽

River System ◽

First Record ◽

Large River ◽

Ecosystem Functions ◽

Macrophyte Species ◽

Saint John ◽

Invasive Aquatic Plants

Eurasian Water-milfoil (Myriophyllum spicatum L.) is regarded by conservation practitioners as one of the most challenging invasive aquatic plants to manage. Owing to its broad tolerance to environmental conditions, vegetative propagation, and rapid establishment and growth, M. spicatum introductions have the potential to drastically alter macrophyte species assemblages via a loss of native species and their respective ecosystem functions. Following the discovery of a single specimen of M. spicatum in the Saint John River, near Fredericton, New Brunswick (Canada) we further investigated the localized distribution of this nonindigenous species. Thirteen areas were identified as potential M. spicatum habitat and were surveyed by wading or snorkeling. Specimens of M. spicatum were collected and morphological identifications were verified through genetic analyses (ITS2; rbcLa). The results of our investigation confirm the presence of M. spicatum at six different locations within the Saint John River. Here we discuss the implications of this discovery in the context of the contiguous aquatic habitats along a large river system.

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