Correlation of serum galactomannan antigen with diagnosis and response to voriconazole in orbital/sino-orbital invasive aspergillosis

Building-work activities could cause dust contamination and dissemination of fungal spores. Significant relationship was found between building-work activities and the incidence of invasive aspergillosis, in highly immunocompromised patients. Renovation-works activities were carried out by four building sites of the hematology ward in a Teaching Hospital without the interruption of clinical activities. These sites were monitored by environmental sampling to determine the particles and fungi count. Clinical surveillance were made using galactomannan antigen test as a proxy for invasive aspergillosis diagnosis. The galactomannan antigen test showed no significant difference between presence (3,85%) or absence (5,76%) of renovation work activities (p=0,497). The particle counts showed higher values of small and big-diameter particles before the renovation works if compared to the end of the activities. It was probably due to the containment measures implemented during and immediately after the final phases of the building site. The Fungi counts showed no particular differences between the phase before and after of the renovation activities. Our finding show that is possible to perform renovation work, during clinical activities, by increasing the clinical and the environmental surveillance.

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Performance of Galactomannan Antigen, Beta-d-Glucan, and Aspergillus-Lateral-Flow Device for the Diagnosis of Invasive Aspergillosis

Indian Journal of Hematology and Blood Transfusion ◽

10.1007/s12288-016-0653-3 ◽

2016 ◽

Vol 33 (1) ◽

pp. 87-92 ◽

Cited By ~ 10

Author(s):

Gökhan Metan ◽

Muzaffer Keklik ◽

Gökçen Dinç ◽

Çiğdem Pala ◽

Afra Yıldırım ◽

...

Keyword(s):

Invasive Aspergillosis ◽

Lateral Flow ◽

Lateral Flow Device ◽

Flow Device ◽

Galactomannan Antigen

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Diagnostic potential of nested PCR, galactomannan EIA, and Beta-D-glucan for invasive aspergillosis in pediatric patients

The Journal of Infection in Developing Countries ◽

10.3855/jidc.2110 ◽

2012 ◽

Vol 6 (04) ◽

pp. 352-357 ◽

Cited By ~ 40

Author(s):

Parisa Badiee ◽

Abdolvahab Alborzi ◽

Mahammad Karimi ◽

Bahman Pourabbas ◽

Pedram Haddadi ◽

...

Keyword(s):

Invasive Aspergillosis ◽

Nested Pcr ◽

Pediatric Patients ◽

Clinical Samples ◽

Predictive Values ◽

Blood Samples ◽

Diagnostic Potential ◽

Increased Risk ◽

Galactomannan Antigen ◽

Noninvasive Tests

Introduction: Limited specific data and investigations are available for invasive aspergillosis (IA) in pediatric patients. We evaluated the diagnostic potential of three noninvasive tests including the Platelia Aspergillus EIA kit for using galactomannan antigen, (1,3)-β-D-glucan Detection Reagent Kit, and nested-PCR for Aspergillus DNA in sera. We evaluated the diagnostic potential of three noninvasive tests including EIA for galactomannan antigen (Platelia Aspergillus), nested PCR assay for Aspergillus DNA and test for (1→3)-β-D-glucan (Glucatell assay Kit). Methodology: All pediatric patients treated at the hematology/oncology unit who were at increased risk of developing invasive aspergillosis were enrolled. Clinical samples were examined for Aspergillus infections by mycological methods. Serial blood samples were collected twice weekly and evaluated by noninvasive tests. Results: We analyzed 230 consecutive blood samples from 62 pediatric patients. The incidence rate of invasive aspergillosis in the patients was found to be 27.4%, and the etiologic agents were Aspergillus flavus, Aspergillus fumigatus, and Aspergillus spp. The sensitivity, specificity, positive and negative predictive values, and likelihood ratios for positive and negative results of galactomannan in patients with proven and probable IA were 90%, 92%, 81.8%, 96%, 11.25, and 0.1; for beta-D-glucan they were 50%, 46%, 26%, 70.6%, 0.9, 0.9; and for nested-PCR they were 80%, 96.2%, 88.9%, 92.6%, 21, and 0.2, respectively. Conclusions: The conventional methods are not able to detect IA, due to the lack of valid and proper sampling. Galactomannan and nested-PCR tests in serum, with enough accuracy and reliability, can serve as noninvasive methods for the detection of IA in pediatric patients. However, the beta-D-glucan test cannot serve as an efficient diagnostic tool in those with hematologic disorders.

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Galactomannan antigen as one of the markers for the diagnosis of invasive aspergillosis

Acta Medica Leopoliensia ◽

10.25040/aml2019.04.057 ◽

2019 ◽

Vol 25 (4) ◽

pp. 57-65

Author(s):

O.O. Yastremska ◽

◽

H.B. Lebed ◽

Keyword(s):

Invasive Aspergillosis ◽

Galactomannan Antigen

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Faculty Opinions recommendation of Aspergillus galactomannan antigen in the bronchoalveolar lavage fluid for the diagnosis of invasive aspergillosis in lung transplant recipients.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1087821.540748 ◽

2007 ◽

Author(s):

Cornelia Lass-Floerl

Keyword(s):

Bronchoalveolar Lavage ◽

Invasive Aspergillosis ◽

Lung Transplant ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Transplant Recipients ◽

Galactomannan Antigen ◽

Lung Transplant Recipients

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Low Sensitivity of the (1-3)-Beta-D-Glucan Assay for Diagnosis of Invasive Aspergillosis in Hematologic Malignancy Patients.

Blood ◽

10.1182/blood.v114.22.2242.2242 ◽

2009 ◽

Vol 114 (22) ◽

pp. 2242-2242 ◽

Cited By ~ 1

Author(s):

Masakatsu Yonezumi ◽

Mitsutoshi Kurosawa ◽

Takashi Fukuhara

Keyword(s):

Invasive Aspergillosis ◽

Conflicts Of Interest ◽

Hematologic Malignancy ◽

Invasive Fungal Disease ◽

Fungal Disease ◽

Negative Group ◽

Test Range ◽

Common Opportunistic Infection ◽

Galactomannan Antigen ◽

Low Sensitivity

Abstract Abstract 2242 Poster Board II-219 Invasive aspergillosis (IA) is a common opportunistic infection in hematologic malignancy patients. The diagnosis of IA is difficult and the outcome is poor despite recent progress in treatment. The detection of galactomannan antigen is useful in diagnosis of IA and is defined as one of EORTC/MSG diagnostic criteria. The (1-3)-beta-D-glucan assay is also used in diagnosis of invasive fungal disease, but the sensitivity and specificity of these tests for IA are variable in recent reports. To evaluate the correlation of them, we analyzed retrospectively the results of these tests in IA cases of hematologic malignancy patients. From January 2006 to December 2008, we reviewed 2914 hematologic malignancy patients (AML=492, ALL=198, MDS=284, NHL=1373, HL=92, MM=375) retrospectively who were recieved chemotherapy or stem cell transplantation(SCT). 607 cases received SCT are included in this study. We diagnosed IA according to the EORTC/MSG criteria, and extracted the data of galactomannan antigen and (1-3)-beta-D-glucan assay examined at diagnosis of each case. 43 cases(1.5%) are diagnosed as invasive fungal disease (Aspergillus=24, Candida=9, Mucor=6, Trichosporon=2, Cryptococcus=1, Geotrichum=1). 23 IA cases are analyzed (proven: 3 cases, probable: 20 cases). All cases are positive for galactomannan antigen test(range, 0.5-5.0< ODI, cut-off value:0.5), but only 9 cases for (1-3)-beta-D-glucan assay (range, 24.5-313pg/ml, cut-off value:20). In the three cases of proven IA, only one case are positive for this assay. There is no difference in patient characteristic and prognosis between the positive and negative group. We conclude that the (1-3)-beta-D-glucan assay is not useful tool for diagnosis of invasive aspergillosis. Disclosures: No relevant conflicts of interest to declare.

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