Effect of seaweed extracts and plant growth regulators on high-frequency in vitro mass propagation of Lycopersicon esculentum L (tomato) through double cotyledonary nodal explant

2011 ◽  
Vol 24 (5) ◽  
pp. 1329-1337 ◽  
Author(s):  
S. Vinoth ◽  
P. Gurusaravanan ◽  
N. Jayabalan
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
High Frequency ◽  
Nodal Explant ◽  
Mass Propagation ◽  
Seaweed Extracts

scholarly journals Induction of Flowering and Fruiting in Plantlets of Tomato (Lycopersicon esculentum Mill.)

2019 ◽  
Vol 2 (2) ◽  
pp. 57-63
Author(s):  
Wina dian Savitri ◽  
Florentinus W.N. Florentinus W.N. Nurtyandi ◽  
Popy Hartatie Hardjo
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Flowering ◽  
Lycopersicon Esculentum Mill ◽  
Hybrid Seeds ◽  
Self Fertilization ◽  
Do So

Tomato (Lycopersicon esculentum Mill.) is used as vegetable or fruit by people around the world. The effective and efficient propagation of tomato is needed due to the high demand, while its availability is not enough to meet the consumer demand. In vitro flowering and fruiting is useful in order to produce high quality hybrid seeds. Also, this can be produced in all seasons. Beside, this technique is beneficial to improve genetic diversity in tomato. In addition, tomato has natures that make it compatible as a plant model. This experiment intended to find the best combination of plant growth regulators or plant retardants  to induce flower and fruit from tomato plantlets. The results will be beneficial to overcome self-fertilization in tomato, as well as to promote higher genetic biodiversity in tomato. To do so, some plant growth regulators (6-Benzylaminopurine (BAP), Indoleacetic acid (IAA), and Gibberellin (GA3)) and retardants (Ancymidol and Paclobutrazol) were used to find the best combination in inducing in vitro flowering and fruiting. The results showed that 1 mg.L-1 BAP was the best candidate plant growth regulator to produce the in vitro flowers and fruits from the treated plantlets.

scholarly journals Influence of growth regulators on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 3) ◽  
pp. 118-122
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová ◽  
Z. Jureková
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Explant Type ◽  
Regeneration Capacity

We studied the effect of different plant growth regulators on in vitro regeneration and plant growth of three cultivars of tomato (Lycopersicon esculentum Mill.) from explants derived from hypocotyls and cotyledons of aseptically grown seedlings. The regeneration capacity was significantly influenced by cultivar and explant type. The highest number of shoots regenerated in both types of explants was recorded on MS medium supplemented with 1.0 mg/dm<sup>3</sup> zeatin and 0.1 mg/dm<sup>3</sup> IAA. The cultivar UC 82 showed the best regeneration capacity on all types of used media. The most responsive explants were hypocotyls with 90&ndash;92% regeneration in dependence on the used cultivars and mean production from 0.18 to 0.38 shoots per explant. &nbsp;

Effect of seaweed liquid extracts and plant growth regulators on in vitro mass propagation of brinjal (Solanum melongena L.) through hypocotyl and leaf disc explants

2014 ◽  
Vol 27 (2) ◽  
pp. 993-1002 ◽  
Author(s):  
Lakkakula Satish ◽  
Ramakrishnan Rameshkumar ◽  
Periyasami Rathinapriya ◽  
Subramani Pandian ◽  
Arokiam Sagina Rency ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Solanum Melongena ◽  
Leaf Disc ◽  
Mass Propagation ◽  
Seaweed Liquid Extracts

scholarly journals Asymbiotic Germination, Effect of Plant Growth Regulators, and Chitosan on the Mass Propagation of Stanhopea hernandezii (Orchidaceae)

2020 ◽  
Vol 98 (4) ◽  
pp. 524-533
Author(s):  
Jesús Arellano-García ◽  
Oswaldo Enciso-Díaz ◽  
Alejandro Flores-Palacios ◽  
Susana Valencia-Díaz ◽  
Alejandro Flores-Morales ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Wild Plants ◽  
Average Height ◽  
Market Demand ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Asymbiotic Germination

Background: Stanhopea hernandezii was collected from natural habitat in Mexico for its beautiful fragrant flowers. Biotechnological strategies of propagation may satisfy the market demand and are useful for conservation programs. Hypothesis: Vigorous seedlings of S. hernandezii can be produced in vitro by asymbiotic seed germination techniques and the addition of chitosan to the culture medium in the temporary immersion system (RITA®) and in semi-solid medium systems. Methods: The first step was the in vitro germination of seeds obtained from a mature capsule of wild plants, followed by multiplication via adventitious protocorm induction known as protocorm-like bodies, using plant growth regulators. For this purpose, we utilized Murashige and Skoog (MS) basal medium amended with 0.5 mg/L ?-Naphthaleneacetic acid, combined with different concentrations of 6-Benzylaminepurine (1, 3, and 5 mg/L). The following step comprised the growth and development of protocorms to obtain plantlets in RITA® flasks containing 250 mL of liquid MS medium combined or not with different chitosan concentrations (5, 10, 15, 20, and 25 mg/L). Results: The results showed that media supplemented with 5, 10, and 15 mg/L chitosan concentrations enabled the obtaining of a larger biomass with a range of 40-48 seedlings/RITA® and an average height of 13 mm. The last step was the development from seedlings into plantlets, the latter being, vigorous and achieving up to 100 % survival after 12 weeks of ex vitro cultivation. Conclusion: This paper describes an efficient process of asymbiotic germination and mass propagation of S. hernandezii, a vulnerable orchid species endemic to Mexico.

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