A simple and cost-effective synthesis of sulfated β-cyclodextrin and its application as chiral mobile phase additive in the separation of cloperastine enantiomers

2021 ◽  
Author(s):  
Krishnapriya Mohanraj ◽  
Krishna Deshpande ◽  
Pranav Pathak ◽  
Vishvas Joshi ◽  
Stephen Barton
Keyword(s):  
Mobile Phase ◽  
Cost Effective ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive ◽  
Effective Synthesis

scholarly journals Enantioselective Chromatographic Analysis of Formoterol Fumarate using Chiral Mobile Phase Additives and Achiral Core-Shell Column

2020 ◽  
Author(s):  
Pranav Pathak ◽  
Krishnapriya Mohanraj
Keyword(s):  
Mobile Phase ◽  
Cost Effective ◽  
Hplc Method ◽  
Enantiomeric Resolution ◽  
Factors Affecting ◽  
Mobile Phase Additives ◽  
Ich Guidelines ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive ◽  
Chiral Mobile Phase Additives

<p>A simple, robust, cost-effective, and rapid RP-HPLC method was developed for the separation of enantiomers of formoterol. The separation was achieved by the chiral mobile phase additive technique on an achiral column. Formoterol is a bronchodilator that consists of 50:50 S, S-formoterol and R, R-formoterol. The bronchodilator activity is attributed to R, R-formoterol. Hence, it is important to develop a method to separate the enantiomers of formoterol. Various factors affecting enantiomeric resolution were investigated and optimized. The enantiomers of formoterol were successfully separated with a resolution of 2.57 with a run-time of 9 minutes. The method was validated in accordance with ICH guidelines. The validated method was successfully applied to the marketed formulation of arformoterol. </p>

A Simple and Cost-Effective Synthesis of Sulfated β-Cyclodextrin and Its Application as Chiral Mobile Phase Additive in the Separation of Cloperastine Enantiomers

2020 ◽  
Author(s):  
Krishna Deshpande ◽  
Pranav Pathak ◽  
Vishvas Joshi ◽  
Stephen barton ◽  
Krishnapriya Mohanraj
Keyword(s):  
Mobile Phase ◽  
Sulfamic Acid ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Enantiomeric Separation ◽  
Reversed Phase ◽  
Recognition Mechanism ◽  
Beta Cyclodextrin ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive

A new, simple and cost-effective method for the synthesis of sulfated beta-cyclodextrin (S-β-CD), one of the most widely used chiral mobile phase additive, using sulfamic acid as sulfonating agent has been described. The method was optimized and the acquired product was characterized and compared with a marketed Sigma Aldrich sulfated beta-cyclodextrin (S-β-CD1). Beta cyclodextrin (β-CD), hydroxypropyl beta-cyclodextrin (HP-β-CD), S-β-CD1 and S-β-CD2 were evaluated as chiral mobile phase additives (CMPAs) for the enantiomeric separation of cloperastine, an antitussive agent, using reversed-phase HPLC. Under the optimized conditions, a resolution of 3.14 was achieved within 15 minutes on an achiral Kromasil C<sub>8</sub> (150 x 4.6 mm, 5 µ) column with a mobile phase of 5mM monopotassium phosphate containing 10mM S-β-CD3 pH 3 and 45% methanol with a run time of 15 min. The method utilizing S-β-CD3 as CMPA was validated as per ICH guidelines and applied for the quantitative determination of cloperastine enantiomers in active pharmaceutical ingredients and pharmaceutical formulations. The selectivity changes imparted by S-β-CD were proven to be beneficial for chiral separation. The chiral recognition mechanism and elution order of the reported enantiomers were determined by simulation studies. It was observed that inclusion complex formation and hydrogen bonding are the major forces for the chiral resolution.

A Simple and Cost-Effective Synthesis of Sulfated β-Cyclodextrin and Its Application as Chiral Mobile Phase Additive in the Separation of Cloperastine Enantiomers

2020 ◽  
Author(s):  
Krishna Deshpande ◽  
Pranav Pathak ◽  
Vishvas Joshi ◽  
Stephen barton ◽  
Krishnapriya Mohanraj
Keyword(s):  
Mobile Phase ◽  
Sulfamic Acid ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Enantiomeric Separation ◽  
Reversed Phase ◽  
Recognition Mechanism ◽  
Beta Cyclodextrin ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive

A new, simple and cost-effective method for the synthesis of sulfated beta-cyclodextrin (S-β-CD), one of the most widely used chiral mobile phase additive, using sulfamic acid as sulfonating agent has been described. The method was optimized and the acquired product was characterized and compared with a marketed Sigma Aldrich sulfated beta-cyclodextrin (S-β-CD1). Beta cyclodextrin (β-CD), hydroxypropyl beta-cyclodextrin (HP-β-CD), S-β-CD1 and S-β-CD2 were evaluated as chiral mobile phase additives (CMPAs) for the enantiomeric separation of cloperastine, an antitussive agent, using reversed-phase HPLC. Under the optimized conditions, a resolution of 3.14 was achieved within 15 minutes on an achiral Kromasil C<sub>8</sub> (150 x 4.6 mm, 5 µ) column with a mobile phase of 5mM monopotassium phosphate containing 10mM S-β-CD3 pH 3 and 45% methanol with a run time of 15 min. The method utilizing S-β-CD3 as CMPA was validated as per ICH guidelines and applied for the quantitative determination of cloperastine enantiomers in active pharmaceutical ingredients and pharmaceutical formulations. The selectivity changes imparted by S-β-CD were proven to be beneficial for chiral separation. The chiral recognition mechanism and elution order of the reported enantiomers were determined by simulation studies. It was observed that inclusion complex formation and hydrogen bonding are the major forces for the chiral resolution.

scholarly journals Enantioselective Chromatographic Analysis of Formoterol Fumarate using Chiral Mobile Phase Additives and Achiral Core-Shell Column

2020 ◽  
Author(s):  
Pranav Pathak ◽  
Krishnapriya Mohanraj
Keyword(s):  
Mobile Phase ◽  
Cost Effective ◽  
Hplc Method ◽  
Enantiomeric Resolution ◽  
Factors Affecting ◽  
Mobile Phase Additives ◽  
Ich Guidelines ◽  
Chiral Mobile Phase Additive ◽  
Mobile Phase Additive ◽  
Chiral Mobile Phase Additives

<p>A simple, robust, cost-effective, and rapid RP-HPLC method was developed for the separation of enantiomers of formoterol. The separation was achieved by the chiral mobile phase additive technique on an achiral column. Formoterol is a bronchodilator that consists of 50:50 S, S-formoterol and R, R-formoterol. The bronchodilator activity is attributed to R, R-formoterol. Hence, it is important to develop a method to separate the enantiomers of formoterol. Various factors affecting enantiomeric resolution were investigated and optimized. The enantiomers of formoterol were successfully separated with a resolution of 2.57 with a run-time of 9 minutes. The method was validated in accordance with ICH guidelines. The validated method was successfully applied to the marketed formulation of arformoterol. </p>

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