Covariates of Cervical Cytokine mRNA Expression by Real-Time PCR in Adolescents and Young Women: Effects of Chlamydia trachomatis Infection, Hormonal Contraception, and Smoking

2006 ◽  
Vol 26 (3) ◽  
pp. 222-232 ◽  
Author(s):  
Mark E. Scott ◽  
Yifei Ma ◽  
Sepideh Farhat ◽  
Stephen Shiboski ◽  
Anna-Barbara Moscicki
Keyword(s):  
Chlamydia Trachomatis ◽  
Mrna Expression ◽  
Real Time ◽  
Young Women ◽  
Real Time Pcr ◽  
Hormonal Contraception ◽  
Cytokine Mrna ◽  
Chlamydia Trachomatis Infection ◽  
Cytokine Mrna Expression

Quantitation of Cytokine mRNA Expression in Cobra Snake Venom Stimulated PBMC of Horses Using Real-Time RT-PCR

2015 ◽  
Vol 9 (6) ◽  
pp. 412-419
Author(s):  
Surasak Akesowan ◽  
Sunutcha Suntrarach ◽  
Suchitra Khunsap ◽  
Wichit Thaveekarn
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Snake Venom ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

Measurement of cytokine mRNA expression in intestinal biopsies of cats with inflammatory enteropathy using quantitative real-time RT-PCR

2006 ◽  
Vol 113 (3-4) ◽  
pp. 404-414 ◽  
Author(s):  
N. Nguyen Van ◽  
K. Taglinger ◽  
C.R. Helps ◽  
S. Tasker ◽  
T.J. Gruffydd-Jones ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

scholarly journals Comparative performance of the Roche COBAS Amplicor assay and an in-house real-time PCR assay for diagnosis of Chlamydia trachomatis infection

2007 ◽  
Vol 56 (3) ◽  
pp. 320-322 ◽  
Author(s):  
Hamid Jalal ◽  
Abdulrahman Al-Suwaine ◽  
Hannah Stephen ◽  
Christopher Carne ◽  
Christopher Sonnex
Keyword(s):  
Chlamydia Trachomatis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Comparative Performance ◽  
Chlamydia Trachomatis Infection ◽  
Roche Cobas

scholarly journals Heterophils isolated from chickens resistant to extra-intestinal Salmonella enteritidis infection express higher levels of pro-inflammatory cytokine mRNA following infection than heterophils from susceptible chickens

2004 ◽  
Vol 132 (6) ◽  
pp. 1029-1037 ◽  
Author(s):  
PAMELA J. FERRO ◽  
CHRISTINA L. SWAGGERTY ◽  
PETE KAISER ◽  
IGAL Y. PEVZNER ◽  
MICHAEL H. KOGUT
Keyword(s):  
Mrna Expression ◽  
Real Time ◽  
Salmonella Enteritidis ◽  
Inflammatory Cytokine ◽  
Rt Pcr ◽  
Cytokine Mrna ◽  
Reciprocal Crosses ◽  
First Report ◽  
Cytokine Mrna Expression

Previous studies showed differences in in vitro heterophil function between parental (A>B) broilers and F1 reciprocal crosses (D>C). Our objectives were to (1) determine if in vitro variations translate to differences in resistance to Salmonella enteritidis (SE) and (2) quantitate cytokine mRNA in heterophils from SE-infected chicks. One-day-old chicks were challenged and organs were cultured for SE. Chicks with efficient heterophils (A and D) were less susceptible to SE compared to chicks with inefficient heterophils (B and C). Heterophils were isolated from SE-infected chicks and cytokine mRNA expression was evaluated using quantitative real-time RT–PCR. Pro-inflammatory cytokine mRNA was up-regulated in heterophils from SE-resistant chicks compared to susceptible chicks. This is the first report to quantitate cytokine mRNA in heterophils from SE-infected chicks. These data show a relationship between in vitro heterophil function, increased pro-inflammatory cytokine mRNA expression, and increased resistance to SE in 1-day-old chicks.

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