The Study on synthesis of chitosan prepared by gamma irradiation and multi-stage deacetylation as chitosan-silica membrane has been carried out. Synthesis of chitosan is carried out by deproteination with papain, demineralization, and deacetylation. Deacetylation of chitosan using gamma irradiation with 0, 5, 10, 15, 20, and 25 kGy and two and multi-stage deacetyllation. The synthesis of chitosan silica composite using sol gel method with the ratio of 1:1; 1:2; and 1:3. Based on the study, gamma irradiation increase deacetylation degree of chitosan and minimize the sodium hydroxide needed for deacetylation chitosan. The multi stage deacetylation increase the efficiency procedure for increase deacetylation degree of chitosan. Chitosan-silica composite has been synthesized by sol gel method. The gamma radiation doses have significantly influence on the deacetylation degree of chitosan. Gamma irradiation and multi stage deacetylation increase the efficiency procedure for preparation chitosan and chitosan-silica composite.
Antibacterial and antibiofilm activities of silver-decorated zinc ferrite nanoparticles synthesized by a gamma irradiation-coupled sol–gel method against some pathogenic bacteria from medical operating room surfaces