An Integrative Single-cell Transcriptomic Atlas of the Post-natal Mouse Mammary Gland Allows Discovery of New Developmental Trajectories in the Luminal Compartment

2021 ◽  
Author(s):  
Martín E. García Solá ◽  
Micaela Stedile ◽  
Inés Beckerman ◽  
Edith C. Kordon
Keyword(s):  
Mammary Gland ◽  
Single Cell ◽  
Developmental Trajectories ◽  
Mouse Mammary Gland ◽  
Luminal Compartment

scholarly journals Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Bhupinder Pal ◽  
Yunshun Chen ◽  
François Vaillant ◽  
Paul Jamieson ◽  
Lavinia Gordon ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Single Cell ◽  
Mouse Mammary Gland ◽  
Rna Profiling

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

2012 ◽  
Vol 34 (3) ◽  
pp. 335-341
Author(s):  
Li-Qiang HAN ◽  
Hong-Ji LI ◽  
Yue-Ying WANG ◽  
Lin-Feng WANG ◽  
Guo-Qing YANG ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mrna Expression ◽  
Mouse Mammary Gland ◽  
Lipogenic Genes
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