Methanol Skin Mucus Extract of Mrigal (Cirrhinus mrigala) Fish Peptide Targeting Viral Particles of Infectious Pancreatic Necrosis Virus (IPNV) and Infectious Salmon Anemia Virus (ISAV): an in silico Approach

2021 ◽  
Vol 27 (2) ◽  
pp. 1429-1440
Author(s):  
Arun Sridhar ◽  
Dinesh Babu Manikandan ◽  
Sathish Kumar Marimuthu ◽  
Manikandan Murugesan ◽  
Thirumurugan Ramasamy
Keyword(s):  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Skin Mucus ◽  
Infectious Salmon Anemia ◽  
Viral Particles ◽  
Salmon Anemia Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Anemia Virus ◽  
Peptide Targeting

scholarly journals Genome Assembly and Particle Maturation of the Birnavirus Infectious Pancreatic Necrosis Virus

2004 ◽  
Vol 78 (24) ◽  
pp. 13829-13838 ◽  
Author(s):  
Rodrigo A. Villanueva ◽  
José L. Galaz ◽  
Juan A. Valdés ◽  
Matilde M. Jashés ◽  
Ana María Sandino
Keyword(s):  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Particle Assembly ◽  
Double Stranded Rna ◽  
Viral Particles ◽  
Agarose Electrophoresis ◽  
Electrophoresis System ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

ABSTRACT In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.

scholarly journals Restriction Fragment Length Polymorphisms and Sequence Analysis: an Approach for Genotyping Infectious Pancreatic Necrosis Virus Reference Strains and Other Aquabirnaviruses Isolated from Northwestern Spain

2004 ◽  
Vol 70 (2) ◽  
pp. 1059-1067 ◽  
Author(s):  
J. M. Cutrín ◽  
J. L. Barja ◽  
B. L. Nicholson ◽  
I. Bandín ◽  
S. Blake ◽  
...  
Keyword(s):  
Pancreatic Necrosis ◽  
Restriction Analysis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Genotype I ◽  
Northwestern Spain ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Reference Strains ◽  
Restriction Fragment Length

ABSTRACT Reference strains of infectious pancreatic necrosis virus resembling the 10 recognized serotypes and local isolates of aquabirnaviruses isolated in northwestern Spain from reservoirs (mollusks) and from asymptomatic and carrier cultured fish were genotyped by restriction fragment length polymorphism (RFLP) and nucleic acid sequence analyses. The RFLP analysis yielded seven genogroups, each of which was clearly correlated with a serotype. Sequence analysis of the three open reading frames provided quite similar results in terms of genogrouping. Based on the results of this study and in order to unify the two types of assays, we propose placing aquabirnaviruses into six genogroups, four of which can be subdivided into two genotypes based on a two-step restriction analysis. The genotyping corresponds with serotyping as follows: genogroup I includes two genotypes corresponding to serotypes A9 (genotype I.1) and A1 (genotype I.2); genogroup II corresponds to serotype A3; genogroup III includes genotypes III.1 (serotype A2) and III.2 (serotype B1); genogroups IV and V include two genotypes, each corresponding to serotypes A5, A6, A7, and A8 (genotypes IV.1, IV.2, V.1, and V.2, respectively);and genogroup VI corresponds to serotype A4. As expected, most local isolates belonged to genotype III.1 and genogroup II. However, a few local isolates corresponded to the American types of genogroup I. Finally, based on the results of this study and due to its simplicity, the two-step restriction analysis assay is proposed as a method for typing new isolates of aquabirnaviruses, and the results correspond to the results of conventional serotyping.

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