Effects of peroxynitrite-induced protein tyrosine nitration on insulin-stimulated tyrosine phosphorylation in HepG2 cells

2009 ◽  
Vol 331 (1-2) ◽  
pp. 49-57 ◽  
Author(s):  
Jun Zhou ◽  
Haidong Li ◽  
Jinhong Zeng ◽  
Kaixun Huang
Keyword(s):  
Tyrosine Phosphorylation ◽  
Hepg2 Cells ◽  
Tyrosine Nitration ◽  
Protein Tyrosine ◽  
Protein Tyrosine Nitration

Diabetes-associated nitration of tyrosine and inactivation of succinyl-CoA:3-oxoacid CoA-transferase

2001 ◽  
Vol 281 (6) ◽  
pp. H2289-H2294 ◽  
Author(s):  
Illarion V. Turko ◽  
Sisi Marcondes ◽  
Ferid Murad
Keyword(s):  
Catalytic Activity ◽  
Matrix Protein ◽  
Ketone Bodies ◽  
Tyrosine Nitration ◽  
Alternative Source ◽  
In Vivo Models ◽  
Protein Tyrosine ◽  
Protein Tyrosine Nitration ◽  
Coa Transferase

High levels of reactive species of nitrogen and oxygen in diabetes may cause modifications of proteins. Recently, an increase in protein tyrosine nitration was found in several diabetic tissues. To understand whether protein tyrosine nitration is the cause or the result of the associated diabetic complications, it is essential to identify specific proteins vulnerable to nitration with in vivo models of diabetes. In the present study, we have demonstrated that succinyl-CoA:3-oxoacid CoA-transferase (SCOT; EC 2.8.3.5 ) is susceptible to tyrosine nitration in hearts from streptozotocin-treated rats. After 4 and 8 wk of streptozotocin administration and diabetes progression, SCOT from rat hearts had a 24% and 39% decrease in catalytic activity, respectively. The decrease in SCOT catalytic activity is accompanied by an accumulation of nitrotyrosine in SCOT protein. SCOT is a mitochondrial matrix protein responsible for ketone body utilization. Ketone bodies provide an alternative source of energy during periods of glucose deficiency. Because diabetes results in profound derangements in myocardial substrate utilization, we suggest that SCOT tyrosine nitration is a contributing factor to this impairment in the diabetic heart.

Biochemistry of Peroxynitrite and Protein Tyrosine Nitration

2018 ◽  
Vol 118 (3) ◽  
pp. 1338-1408 ◽  
Author(s):  
Gerardo Ferrer-Sueta ◽  
Nicolás Campolo ◽  
Madia Trujillo ◽  
Silvina Bartesaghi ◽  
Sebastián Carballal ◽  
...  
Keyword(s):  
Tyrosine Nitration ◽  
Protein Tyrosine ◽  
Protein Tyrosine Nitration

Protein tyrosine nitration and peroxynitrite

2002 ◽  
Vol 16 (9) ◽  
pp. 1144-1144 ◽  
Author(s):  
William M. Deen ◽  
Steven R. Tannenbaum ◽  
Joseph S. Beckman
Keyword(s):  
Tyrosine Nitration ◽  
Protein Tyrosine ◽  
Protein Tyrosine Nitration
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