The anti-tumour effect of induced pluripotent stem cells against submandibular gland carcinoma in rats is achieved via modulation of the apoptotic response and the expression of Sirt-1, TGF-β, and MALAT-1 in cancer cells

2021 ◽  
Author(s):  
Eman Mohamed Faruk ◽  
Dina Sabry ◽  
Ahmed A. Morsi ◽  
Yasmine Alaa El-Din ◽  
Neama M. Taha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cancer Cells ◽  
Submandibular Gland ◽  
Pluripotent Stem Cells ◽  
Apoptotic Response ◽  
Gland Carcinoma ◽  
Induced Pluripotent ◽  
Anti Tumour Effect

scholarly journals Role of LncRNA MALAT-1 on Induced Submandibular Gland Carcinoma in Albino Rats Treated with Induced Pluripotent Stem Cells (Histological and Immunhistochemical Study)

2019 ◽  
pp. 1-15
Author(s):  
Eman Mohamed Faruk ◽  
Sherifa Abd El-Salam ◽  
Dina Sabry ◽  
Engy Medhat ◽  
Yasmine Alaa El-Din
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Induced Pluripotent Stem Cells ◽  
Submandibular Gland ◽  
Pluripotent Stem Cells ◽  
Treated Group ◽  
Albino Rats ◽  
Group Iii ◽  
Gland Carcinoma ◽  
Induced Pluripotent

Salivary gland tumors show complex histopathology and the treatment depends mainly on the stage of cancer. Induced pluripotent stem cells (iPS) have a great role in regenerative medicine as they can generate pluripotent stem cells from any available cell types as fibroblast. Thus, the aim of this work is to investigate the possible therapeutic effect of (iPS) on induced salivary gland cancer through evaluation of the silent information regulators of sirtuin-1 (Sirt-1), Tgf-β genes and their protein expressions in addition to LncRNA MALAT-1 expression. Thirty male albino rats were employed and divided into three groups (ten rats for each group), group 1 (control): Rats were injected with phosphate buffered saline (PBS), group II induced squamous cell carcinoma (SCC): rats were injected with squamous carcinoma cells (SCC), group III (induced SCC/iPS): SCC treated rats treated with 5 × 106 iPS cells. Submandibular specimens were taken and prepared for histological, histochemical and immunohistochemical studies for Bax and TGF-β3 protein. Also, Real time PCR was performed for Sirt-1, Tgf-β, and MALAT-1 LncRNA genes expression. SIRT-1 and TGF-β protein level expression was assessed by western blot technique. Group III (iPS treated group) revealed more or less normal acinar structure with normal rearrangement of acini and normal intralobular ducts with an increase in their number. In the iPS treated group there was increasing in the amount of mucopoly saccharide in the acinar cells and intensity of BAX immunostaining while, TGF-β3 was decreased in its   intensity in comparison to that of the cancer treated group.  In addition to Sirt-1, Tgf-β, and MALAT-1 LncRNA expressions were increased in cancer group compared to iPS treated and control groups. Induced pluripotent stem cells play a potential therapeutic role in treatment of induced submandibular gland carcinoma.

scholarly journals Advances and Challenges on Cancer Cells Reprogramming Using Induced Pluripotent Stem Cells Technologies

2016 ◽  
Vol 7 (15) ◽  
pp. 2296-2303 ◽  
Author(s):  
Diana Aparecida Dias Câmara ◽  
Lisley Inata Mambelli ◽  
Allan Saj Porcacchia ◽  
Irina Kerkis
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cancer Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Reprogramming of Cancer Cells into Induced Pluripotent Stem Cells Questioned

2019 ◽  
Vol 12 (3) ◽  
pp. 430-439
Author(s):  
Jin Seok Bang ◽  
Na Young Choi ◽  
Minseong Lee ◽  
Kisung Ko ◽  
Yo Seph Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cancer Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Directed differentiation of mouse-induced pluripotent stem cells generates dopaminergic nerve

2010 ◽  
Vol 34 (8) ◽  
pp. S36-S36
Author(s):  
Ping Duan ◽  
Xuelin Ren ◽  
Wenhai Yan ◽  
Xuefei Han ◽  
Xu Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Induced Pluripotent
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