The gene PmYB confers broad-spectrum powdery mildew resistance in the multi-allelic Pm2 chromosome region of the Chinese wheat cultivar YingBo 700

2015 ◽  
Vol 35 (5) ◽  
Author(s):  
Pengtao Ma ◽  
Hongxia Zhang ◽  
Hongxing Xu ◽  
Yunfeng Xu ◽  
Yanwei Cao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Wheat Cultivar ◽  
Chromosome Region ◽  
Mildew Resistance ◽  
Chinese Wheat Cultivar ◽  
Chinese Wheat

Characterization of PmHHXM, a New Broad-spectrum Powdery Mildew Resistance Gene in Chinese Wheat Landrace Honghuaxiaomai

Plant Disease ◽  
2021 ◽  
Author(s):  
Shulin Xue ◽  
Mingxue Lu ◽  
Shanshan Hu ◽  
Hongxing Xu ◽  
Yuyu Ma ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Chinese Wheat

Powdery mildew, caused by fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is one of agronomically important and widespread wheat diseases causing severe yield losses. Deployment of broad‐spectrum disease-resistance genes is the preferred strategy to prevent this pathogen. Chinese wheat landrace Honghuaxiaomai (HHXM) was resistant to all 23 tested Bgt isolates at the seedling stage. The F1, F2, and F2:3 progenies derived from the cross HHXM × Yangmai 158 were used in this study, and genetic analysis revealed that a single dominant gene, designated as PmHHXM, conferred resistance to Bgt isolate E09. Bulked segregant analysis and molecular mapping initially located PmHHXM to the distal region of chromosome 4AL. To fine map PmHHXM, two critical recombinants were identified from 592 F2 plants and delimited PmHHXM to a 0.18-cM Xkasp475200–Xhnu552 interval covering 1.77-Mb, in which a number of disease resistance-related gene clusters were annotated. Comparative mapping of this interval revealed a perturbed synteny among Triticeae species. This study reports the new powdery mildew resistance gene PmHHXM that seems different from three known QTL/genes identified on chromosome 4AL and has significant values for further genetic improvement. Analysis of the polymorphisms of 13 co-segregating markers between HHXM and 170 modern wheat cultivars indicates that Xhnu227 and Xsts478700 developed here are ideal for marker-assisted introgression of this resistance gene in wheat breeding.

scholarly journals Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2645-2651 ◽  
Author(s):  
Yanmin Qie ◽  
Yuan Sheng ◽  
Hongxing Xu ◽  
Yuli Jin ◽  
Feifei Ma ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Recessive Gene ◽  
Genetic Distances ◽  
Linked Gene ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Allelism Tests ◽  
Wheat Lines ◽  
Chinese Wheat

Chinese wheat landrace Dahongtou was resistant to 35 of 38 tested Chinese isolates of Blumeria graminis f. sp. tritici at the seedling stage. Genetic analysis of the F2 populations and their derived F2:3 families of crosses of Dahongtou with the susceptible varieties Mingxian 169 and Huixianhong indicated that the resistance of Dahongtou to B. graminis f. sp. tritici isolate E09 was conferred by a single recessive gene, tentatively designated as pmDHT. The gene was mapped to chromosome arm 7BL and flanked by markers Xwmc526/XBE443877 and Xgwm611/Xwmc511 at genetic distances of 0.8 and 0.3 cM, respectively. The chromosomal position of pmDHT was similar to the multi-allelic Pm5 locus on 7BL. Allelism tests with crosses of Dahongtou with Fuzhuang 30 (Pm5e) and Xiaobaidong (mlxbd) indicated that pmDHT was allelic to both Pm5e and mlxbd. However, pmDHT showed a different pattern of resistance to the 38 B. graminis f. sp. tritici isolates compared with wheat lines with Pm5a, Pm5b, Pm5e, mlxbd, and PmHYM and also differed from PmSGA. Thus, pmDHT was identified most likely as a new allele or at least a closely linked gene of the Pm5 locus. This gene can be transferred into susceptible wheat cultivars/lines and pyramided with other resistance genes through marker-assisted selection to improve powdery mildew resistance.

scholarly journals Naturally Occurring Broad-Spectrum Powdery Mildew Resistance in a Central American Tomato Accession Is Caused by Loss of Mlo Function

2008 ◽  
Vol 21 (1) ◽  
pp. 30-39 ◽  
Author(s):  
Yuling Bai ◽  
Stefano Pavan ◽  
Zheng Zheng ◽  
Nana F. Zappel ◽  
Anja Reinstädler ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Candidate Gene Approach ◽  
Loss Of Function ◽  
Virus Induced Gene Silencing ◽  
Coding Region ◽  
Oidium Neolycopersici ◽  
Tomato Cultivar ◽  
Mildew Resistance

The resistant cherry tomato (Solanum lycopersicum var. cerasiforme) line LC-95, derived from an accession collected in Ecuador, harbors a natural allele (ol-2) that confers broad-spectrum and recessively inherited resistance to powdery mildew (Oidium neolycopersici). As both the genetic and phytopathological characteristics of ol-2–mediated resistance are reminiscent of powdery mildew immunity conferred by loss-of-function mlo alleles in barley and Arabidopsis, we initiated a candidate-gene approach to clone Ol-2. A tomato Mlo gene (SlMlo1) with high sequence-relatedness to barley Mlo and Arabidopsis AtMLO2 mapped to the chromosomal region harboring the Ol-2 locus. Complementation experiments using transgenic tomato lines as well as virus-induced gene silencing assays suggested that loss of SlMlo1 function is responsible for powdery mildew resistance conferred by ol-2. In progeny of a cross between a resistant line bearing ol-2 and the susceptible tomato cultivar Moneymaker, a 19-bp deletion disrupting the SlMlo1 coding region cosegregated with resistance. This polymorphism results in a frameshift and, thus, a truncated nonfunctional SlMlo1 protein. Our findings reveal the second example of a natural mlo mutant that possibly arose post-domestication, suggesting that natural mlo alleles might be evolutionarily short-lived due to fitness costs related to loss of mlo function.

scholarly journals Mapping Powdery Mildew Resistance Gene pmYBL on Chromosome 7B of Chinese Wheat (Triticum aestivum L.) Landrace Youbailan

Plant Disease ◽  
2020 ◽  
Vol 104 (9) ◽  
pp. 2411-2417
Author(s):  
Xiaodan Xu ◽  
Wei Liu ◽  
Zhiyong Liu ◽  
Jieru Fan ◽  
Yilin Zhou
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Recessive Gene ◽  
Triticum Aestivum L ◽  
Genetic Distances ◽  
Genomic Region ◽  
Snp Markers ◽  
Single Nucleotide ◽  
Mildew Resistance ◽  
Chinese Wheat

Chinese wheat landrace Youbailan has excellent resistance to powdery mildew caused by Blumeria graminis f. sp. tritici. In the present study, genetic analysis indicated that a recessive gene, tentatively designated pmYBL, was responsible for the powdery mildew resistance of Youbailan. pmYBL was located in the 695-to-715-Mb genomic region of chromosome 7BL, with 19 gene-linked single-nucleotide polymorphism (SNP) markers. It was flanked by SNP1-12 and SNP1-2 with genetic distances of 0.6 and 1.8 centimorgans, respectively. The disease reaction patterns of Youbailan and four cultivars (lines) carrying the powdery mildew (Pm) genes located on chromosome arm 7BL indicated that pmYBL may be allelic or closely linked to these genes. All of the SNP markers linked to pmYBL were diagnostic, indicating that these markers will be useful for pyramiding pmYBL using marker-assisted selection.

Characterization of PmDGM conferring powdery mildew resistance in Chinese wheat landrace Duanganmang

Plant Disease ◽  
2021 ◽  
Author(s):  
Yanan Wu ◽  
Xiaoting Yu ◽  
Xu Zhang ◽  
Lijuan Yan ◽  
Li Gao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Single Nucleotide Polymorphism Array ◽  
Single Nucleotide ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Yield And Quality ◽  
Genome Homology ◽  
Chinese Wheat

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease threatening yield and quality. Host resistance is considered the most effective and preferred means to control this disease. Wheat landrace Duanganmang (DGM) showed high resistance or near immunity to Bgt mixture from Henan province, China. DGM was crossed with highly susceptible Chinese wheat landrace Huixianhong (HXH) and cultivar Shimai 15 (SM15) to produce genetic populations. The resistance of DGM to Bgt isolate E09 was shown to be controlled by a single dominant Mendelian factor, tentatively designated PmDGM. Marker analysis and 55K SNP (single nucleotide polymorphism) array scanning showed that this gene was positioned in the Pm5 interval (2.4 cM or 1.61 Mb) flanked by Xhenu099 and Xmp1158 in the Chinese Spring reference genome. Homology-based cloning and sequence analysis demonstrated that DGM has the identical NLR gene (Pm5e) and RXL gene reported in Fuzhuang 30 (FZ30) conferring and modifying the powdery mildew resistance, respectively. However, based on the different reaction patterns to the Bgt isolate B15 between DGM and FZ30, we speculate that DGM may have two tightly linked genes that could not be separated in the current mapping population, one is PmDGM and the other is Pm5e. Hence, this study provides a valuable resistance resource for improvement of powdery mildew resistance.

Sign in / Sign up

Export Citation Format

Share Document