Caffeine does not enhance radiosensitivity of normal liver tissue in vivo

2010 ◽  
Vol 38 (7) ◽  
pp. 4359-4367 ◽  
Author(s):  
Tie-Jun Wang ◽  
Zhong-Shan Liu ◽  
Zhao-Chong Zeng ◽  
Shi-Suo Du ◽  
Ming Qiang ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Normal Liver ◽  
Normal Liver Tissue

scholarly journals Effect of Intrahepatic Arterial Delivery of Sorafenib on Normal Liver Tissue of Rabbit: An Experimental Study

2020 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Eerdunbagena Ning ◽  
Zhijun Wang
Keyword(s):  
Heart Rate ◽  
Rectal Temperature ◽  
Liver Tissue ◽  
High Performance ◽  
Arterial Embolization ◽  
Hepatic Arterial Infusion ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
Breathing Rate ◽  
Local Necrosis

Objective: To assess the safety, feasibility and eluting efficiency of intrahepatic arterial delivery of sorafenib on normal liver tissue of rabbit. Methods: 24 New Zealand rabbits were randomly divided into three groups: group Ⅰ (Lipiodol-sorafenid), group Ⅱ (Lipiodol) and group Ⅲ (Sorafenib). Group Ⅰ and Ⅱ were treated by transcatheter selective hepatic arterial embolization with emulsions of lipiodol and sorafenib or with only lipiodol, while group Ⅲ was given hepatic arterial infusion with sorafenib. Sorafenib concentration in plasma was determined by HPLC (high performance liquid chromatography) in 0 min, 20 min, 1h, 2h, 4h, 8h, 16h, 32h and 48h respectively. The breathing rate, heart rate, rectal temperature and body weight were measured, as well the blood routine test and the function of liver, kidney, and heart. Two animals of each group were respectively killed in the 3rd day, 1st, 3rd and 6th week after treatment. Histopathologic study was done to liver, heart, kinney, lung, brain, gall bladder and intestine. Result: ① The peak sorafenib concentration (Cmax)and AUC(Area under curve) in plasma in groupⅠwas 2.46±0.101μg/ml and 945.72 ± 52.3 μg/mL.min respectively, while in group Ⅲ which was 3.78±0.180 ug/ml and 546.98±21.1μg/mL.min. Compared with groupⅢ, the Cmax and AUC of groupⅠhad a significant statistics difference (p<0.05). ② The breathing rate, heart rate, rectal temperature and AST/ALT,WBC,NEU% of group Ⅰand groupⅢhas a significant statistics difference(p<0.05) in the 3rd day. ③CK ,CK-MB, DB, Cr，BUN,RBC,PLT in plasma did not change in all group.④Local necrosis was seen in group Ⅰand group Ⅱin the 3rd day and 1st week, but they did not seem to be different. Group Ⅲ showed no necrosis. Granulation tissue with bile duty, portal vein and microfossils hyperplasia were seen in local necrosis area in the 3rd week. No pathological changes were found in brain, heart, kidney, intestine and gallbladder. Conclusion: TAE with emulsions of lipiodol and sorafenib is feasible, safe and has some slow-release effect.

Normal liver tissue change after proton beam therapy

2018 ◽  
Vol 36 (9) ◽  
pp. 559-565 ◽  
Author(s):  
Nobuyoshi Fukumitsu ◽  
Shinsei Takahashi ◽  
Toshiyuki Okumura ◽  
Toshiki Ishida ◽  
Keiko Nemoto Murofushi ◽  
...  
Keyword(s):  
Proton Beam ◽  
Liver Tissue ◽  
Normal Liver ◽  
Proton Beam Therapy ◽  
Normal Liver Tissue ◽  
Tissue Change

Accuracy of real-time shear wave elastography in the assessment of normal liver tissue in the guinea pig (cavia porcellus)

2017 ◽  
Vol 20 (1) ◽  
pp. 51-56
Author(s):  
K. Glińska-Suchocka ◽  
K. Kubiak ◽  
J. Spużak ◽  
M. Jankowski ◽  
P. Borusewicz
Keyword(s):  
Guinea Pig ◽  
Real Time ◽  
Shear Wave ◽  
Liver Tissue ◽  
Ultrasound Image ◽  
Shear Wave Elastography ◽  
Normal Liver ◽  
Normal Liver Tissue ◽  
Invasive Technique ◽  
Non Invasive

Abstract Shear wave elastography is a novel technique enabling real-time measurement of the elasticity of liver tissue. The color map is superimposed on the classic ultrasound image of the assessed tissue, which enables a precise evaluation of the stiffness of the liver tissue. The aim of the study was to assess the stiffness of normal liver tissue in the guinea pig using shear wave elastography. The study was carried out on 36 guinea pigs using the SuperSonic Imagine Aixplorer scanner, and a 1 to 6 MH convex SC6-1 transducer. An ultrasound guided Try-Cut liver core needle biopsy was carried out in all the studied animals and the collected samples were examined to exclude pathological lesions. The mean liver tissue stiffness ranged from 0.89 to 5.40 kPa. We found that shear wave elastography is an easy, non-invasive technique that can be used to assess the stiffness of liver tissue. The obtained results can be used in future studies to assess the types and changes of liver tissue in the course of various types of liver disease.

Caffeine Biotransformation in Human Hepatocyte Lines Derived from Normal Liver Tissue

1994 ◽  
Vol 201 (2) ◽  
pp. 559-566 ◽  
Author(s):  
E.A. Roberts ◽  
K.N. Furuya ◽  
B.K. Tang ◽  
W. Kalow
Keyword(s):  
Liver Tissue ◽  
Normal Liver ◽  
Human Hepatocyte ◽  
Normal Liver Tissue

scholarly journals Hepatostat: Liver regeneration and normal liver tissue maintenance

Hepatology ◽  
2017 ◽  
Vol 65 (4) ◽  
pp. 1384-1392 ◽  
Author(s):  
George K. Michalopoulos
Keyword(s):  
Liver Regeneration ◽  
Liver Tissue ◽  
Normal Liver ◽  
Normal Liver Tissue

Signal Suppression of Normal Liver Tissue by Phase Corrected Inversion Recovery

1989 ◽  
Vol 13 (4) ◽  
pp. 650-655 ◽  
Author(s):  
Bernt Lehmann ◽  
Ezio Fanucci ◽  
Francesco Gigli ◽  
Detlev Uhlenbrock ◽  
Carlo Bartolozzi
Keyword(s):  
Liver Tissue ◽  
Normal Liver ◽  
Inversion Recovery ◽  
Normal Liver Tissue ◽  
Signal Suppression

scholarly journals Cardiac impact of high-frequency irreversible electroporation using an asymmetrical waveform on liver in vivo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jing Li ◽  
Jingjing Wang ◽  
Xiaobo Zhang ◽  
Xiao Zhang ◽  
Hongmei Gao ◽  
...  
Keyword(s):  
Muscle Relaxant ◽  
High Frequency ◽  
Liver Tissue ◽  
Irreversible Electroporation ◽  
Normal Liver ◽  
Electrical Signal ◽  
Cardiac Complications ◽  
Cardiac Damage ◽  
Electrical Pulses

Abstract Background High-Frequency Irreversible Electroporation (H-FIRE) is a novel technology for non-thermal ablation. Different from Irreversible electroporation (IRE), H-FIRE delivers bipolar electrical pulses without muscle contraction and does not cause electrolysis. Currently, little is known regarding the cardiac safety during the administration of H-FIRE on liver. The aim of this study was to evaluate the changes of electrocardiogram (ECG) and biomarkers of cardiac damage during asymmetrical waveform of H-FIRE therapy in vivo. Methods The swines (n = 7) in IRE group, which used 100 pulses (2200 V, 100–100 μs configuration), were administrated with muscle relaxant under anesthesia. In the absence of muscle relaxant, 7 swines in H-FIRE group were performed with 2400 pulses (3000 V, 5–3–3–5 μs configuration). Midazolam (0.5 mg/kg) and xylazine hydrochloride (20 mg/kg) were given to induce sedation, followed by Isoflurane (2.5%, 100% oxygen, 3 L/min) to maintain sedation in all the swines. Limb lead ECG recordings were analyzed by two electrophysiologists to judge the arrhythmia. Cardiac and liver tissue was examined by pathology technique. Results The ablation zones were larger in H-FIRE than IRE. Both IRE and H-FIRE did not affect the autonomous cardiac rhythm. Even when the electrical signal of IRE and H-FIRE fell on ventricular vulnerable period. Moreover, cTnI in IRE group showed an increase in 4 h after ablation, and decreased to baseline 72 h after ablation. However, cTnI showed no significant change during the administration of H-FIRE. Conclusions The study suggests an asymmetrical waveform for H-FIRE is a promising measure for liver ablation. The results were based on normal liver and the swines without potential cardiac diseases. With the limitations of these facts, asymmetrical waveform for H-FIRE of liver tissue seems relatively safe without major cardiac complications. The safety of asymmetrical waveform for H-FIRE needs to evaluate in future.

Effect of Rho/ROCK pathway inhibition on metastasis-free and overall survival in biomarker selected, orthotopic, patient-derived models of pancreatic cancer.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e15759-e15759
Author(s):  
Venessa T. Chin ◽  
Adnan Nagrial ◽  
James Conway ◽  
Claire Vennin ◽  
Lorraine A. Chantrill ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Overall Survival ◽  
Liver Tissue ◽  
Cellular Proliferation ◽  
Imaging System ◽  
Normal Liver ◽  
Human Condition ◽  
Normal Liver Tissue ◽  
Saline Control ◽  
Cd 31

e15759 Background: PC is a highly lethal disease which metastasises early. The sequencing efforts of the Australian Pancreatic Cancer Genome Initiative (APGI) identified ROCK-1 as a target of interest. The Rho/ROCK pathway is essential in cellular movement, metastasis, vasculogenesis and stromal signaling. Fasudil (F) is a ROCK inhibitor which has been shown to be safe for human use. Using unique, patient derived models, we have previously shown that F plus gemcitabine (G) does not reduce cellular proliferation in vitro but reduces tumour size and improves overall survival (OS) in subcutaneous patient-derived xenografts (PDX). However, PDXs are critisised for not fully recapitulating the human condition. Here we aimed to generate a patient-derived orthotopic xenograft (O-PDX) which could be used to assess the effects of F + G on metastasis formation and OS. Methods: One patient-derived cell line (PDCL) was labled with firefly luciferase. This was injected into the pancreas of NOD/Shi- scid/IL-2Rγnull (NSG) mice to generate the O-PDX model. The O-PDX was imaged weekly with the IVIS imaging system. The O-PDXs were treated with saline control (S) (n = 8), gemcitabine (G) (n = 10) fasudil (F) (n = 6) or gemcitabine + fasudil (GF) (n = 10). Mice were culled once radiographic or clinical evidence of ascites was detected. Metastasis free (MFS) and OS were recorded. Vessel quantification was performed via CD-31 staining. Results: Metastases seen (spleen, liver, lung, ovary) on imaging were confirmed using GFP stains on immunohistochemistry. F prolonged MFS but not OS when compared with S. GF significantly prolonged MFS (33.5 versus 43 days; p = 0.0339) and OS (51.5 versus 64 days; p = 0.0035). CD-31 staining showed that treatment with F resulted in increased vascularity in the liver metastases and normal liver tissue but not in the pancreas tumour. Conclusions: The addition of Fasudil to gemcitabine improves MFS and OS in an orthotopic model of PC and is a promising new therapeutic option. The increase in vascularity of the metastases and normal liver tissue suggest that changes in vascularity are important and may result in improved delivery of gemcitabine.

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