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2022 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
Chance S. Friesen ◽  
William San Pablo ◽  
Julie Bass ◽  
Uttam Garg ◽  
Jennifer M. Colombo

Background: Disaccharidase (DS) deficiencies have been reported in pediatric patients with inflammatory bowel disease (IBD), but the relationship between duodenal inflammation and DS deficiency has not been evaluated outside of lactase deficiency. Methods: This study assessed DS levels and DS deficiencies in pediatric IBD patients who underwent endoscopy with assessment of DS activity. Records were reviewed for IBD subtype, pathology findings, and the results of DS analysis. Results: A total of 136 patients were identified. Overall, 89 (65.4%) patients had a diagnosis of Crohn’s disease (CD), 31 (22.8%) patients had a diagnosis of ulcerative colitis (UC), and 16 (11.8%) patients had a diagnosis of indeterminant colitis. Lactase deficiency was identified in 55.9% of patients, followed by maltase deficiency (19.9%), sucrase and palatinase deficiency (14%), and pan-deficiency (12.5%). When analyzing only patients with CD, patients with duodenitis were more likely to exhibit sucrase deficiency, palatinase deficiency, and pan-deficiency with a trend towards maltase deficiency. Conclusions: The most common DS deficiency was lactase deficiency; however, this was not related to duodenal inflammation. Pediatric patients with CD and duodenal inflammation exhibit DS deficiencies, namely, sucrase, palatinase, and pan-deficiency. Dietary adjustments may be warranted temporarily until duodenal inflammation is healed in patients with CD and duodenitis.


2021 ◽  
Vol 11 ◽  
Author(s):  
Zong-Tao Chai ◽  
Xiu-Ping Zhang ◽  
Jian-Yang Ao ◽  
Xiao-Dong Zhu ◽  
Meng-Chao Wu ◽  
...  

Portal vein tumor thrombus (PVTT) is one of the most serious forms of hepatocellular carcinoma (HCC) vessel metastasis and has a poor survival rate. However, the molecular mechanism of PVTT has not yet been elucidated. In this study, the molecular mechanism of AXL expressed in tumor-derived endothelial cells (TECs) in vessel metastasis was investigated. High AXL expression was observed in TECs, but not in the tumor cells of HCC patients with PVTT and this was associated with poor overall survival (OS) and disease-free survival (DFS). AXL overexpression was positively associated with CD 31 expression both in vitro and in vivo. AXL promoted the cell proliferation, tube formation, and migration of both TECs and normal endothelial cells (NECs). High expression of AXL in TECs promoted the cell migration, but not the proliferation of HCC cells. Further studies demonstrated that AXL promoted cell migration and tube formation through activation of the PI3K/AKT/SOX2/DKK-1 axis. AXL overexpression in HUVECs promoted tumor growth and liver or vessel metastasis of HCC in xenograft nude mice, which could be counteracted by treatment with R428, an AXL inhibitor. R428 reduced tumor growth and CD 31 expression in HCC in PDX xenograft nude mice. Therefore, AXL over-expression in TECs promotes vessel metastasis of HCC, which indicates that AXL in TECs could be a potential therapeutic target in HCC patients with PVTT.


Author(s):  
Diogo Assis Souza ◽  
Maria Paula Meireles Fenelon ◽  
Isaac Azevedo Silva
Keyword(s):  
Cd 31 ◽  

A cirurgia de revascularização de miocárdio foi desenvolvida para tratamento de doença arterial coronariana (DAC), em meados nos anos 60, com enxerto, inicialmente de veia safena e, posteriormente, com a artéria torácica interna (ATI), que mudou por completo o tratamento de DAC e o prognóstico dos pacientes. Para ter o máximo em resultados, a qualidade do enxerto é de extrema importância, incluindo as técnicas para dissecção. Muitas técnicas para diminuir a lesão tecidual tem sido estudadas na busca de aumentar a patência vascular. O uso de laser de diodo é uma alternativa para a extração da veia safena. Objetivo: Comparar o grau de lesão tecidual provocado pelo laser de diodo na dissecção da veia safena e da ATI com o grau de lesão tecidual provocado pelo uso do eletrocautério. Metodologia: É um estudo prospectivo e randomizado que comparou o uso de laser de diodo e o uso de eletrocautério na dissecção da veia safena e da ATI em cirurgias de revascularização do miocárdio no período entre janeiro e junho de 2019. A amostra foi composta de 18 pacientes divididos em quatro grupos: Grupo A1: uso de laser de diodo na dissecção de ATI. Grupo A2: uso de laser de diodo na dissecção de veia safena. Grupo B1: uso de eletrocautério na dissecção de ATI. Grupo B2: uso de eletrocautério na dissecção de veia safena. Foram retirados fragmentos da veia safena e da ATI para um estudo de imuno-histoquímica com marcadores CD-31 e CD-34 para avaliação de processo inflamatório, que diminui a patência do enxerto no médio e longo prazo. Resultados: O estudo foi realizado em dezoito pacientes divididos em quatro grupos. O Grupo A1 é o grupo teste (laser de diodo) de dissecção de ATI e foi formado por 5 pacientes (4 do sexo masculino e 1 do sexo feminino) com idade entre 57 e 71 anos (média 62,6 + 6,11). O Grupo A2 é o grupo teste (laser de diodo) de dissecção de veia safena e foi formado por 3 pacientes (1 do sexo masculino e 2 do sexo feminino) com idade entre 61 e 80 anos (média 68,3 + 8,34). O Grupo B1 é o grupo controle (eletrocautério) de dissecção de ATI e foi formado por 5 pacientes (2 do sexo masculino e 3 do sexo feminino) com idade entre 44 e 80 anos (média 61,8 anos ±13,28) e o Grupo B2 é o grupo controle (eletrocautério) de dissecção de veia safena e foi formado por 5 pacientes (1 do sexo masculino e 4 do sexo feminino) com idade entre 44 e 71 anos (média 58,25 + 9,69). Conclusão: Não foi observado variação de lesão tecidual entre o uso de laser de diodo e eletrocautério, mostrando ser factível o uso do laser de diodo para extração de enxerto de veia safena na utilização para cirurgia de revascularização do miocárdio.


Author(s):  
С.М. Діденко
Keyword(s):  

Вступ. Протягом останніх десятиліть захворюваність на цукровий діабет ЦД прийняла масштаби неінфекційної пандемії. Поширеність ЦД кожні 10–15 років подвоюється. Більше ніж у половини хворих виникає діабетична мікро-макроангіопатія. Це ускладнення ЦД пов’язують з високим ризиком розвитку і швидкого прогресування судинної патології. Мета дослідження. Проаналізувати результати дослідження гемомікроциркуляторного русла (ГМЦР) при хронічній критичній ішемії нижньої кінцівки (ХКІНК) у хворих на цукровий діабет (ЦД). Розробити методику розрахунку показника ступеня діабетичної мікроангіопатії (ПСДМ) на підставі визначення кількісних характеристик ураження ГМЦР шкіри у хворих на ЦД із ХКІНК. Матеріали та методи. Були відібрані зразки парафінових блоків шкіри 63 хворих (дослідна група) на ЦД, тип ІІ з ХКІНК на тлі поєднаного стенотично-оклюзійного ураження артерій стегнового та підколінно-гомілкового сегментів в поєднанні з виразково-некротичним ураженням стопи, які проходили лікування в Центрі судинної хірургії Клінічної лікарні «ФЕОФАНІЯ» Державного управління справами протягом 2013–2017 рр. Групою порівняння були вибрані зразки шкіри та м’язів 30 пацієнтів, що не мали системних захворювань. Проводили гістологічні та імуногістохімічні дослідження для виявлення колагену IV – маркера базальних мембран, VEGF – васкулярного ендотеліального росткового фактора; CD 31 – ендотеліального фактора; гладком’язового актину. Вимірювали та порівнювали діаметр кровоносних судин ГМЦР шкіри та м’язів, щільність капілярів на одиницю площі. На підставі отриманих даних проводили розрахунок ПСДМ. Результати досліджень та їх обговорення. Були виявлені характерні зміни морфометричних показників судин ГМЦР шкіри у хворих на ЦД, тип ІІ з хронічною критичною ішемією нижньої кінцівки. Розроблена методика розрахунку ПСДМ. Виявлена кореляція між ступенем діабетичної мікроангіопатії ймовірністю тромбозу після гібридної артеріальної реконструкції та розроблений алгоритм обстеження хворих в післяопераційному періоді. Висновки. Сукупність отриманих даних про якісні та кількісні зміни ГМЦР шкіри дозволила розробити критерії для розрахунку ПСДМ. Виявлена кореляція між ступенем діабетичної мікроангіопатії та ймовірністю тромбозу після гібридної артеріальної реконструкції. Визначення ступеня тяжкості діабетичної мікроангіопатії може бути використане при створенні алгоритму обстеження для встановлення показань до превентивних операцій, що попереджують тромбоз зони артеріальної реконструкції (ЗАР) та характеру консервативного лікування після артеріальних реконструкцій у хворих на ЦД із ХКІНК. Ключові слова: цукровий діабет, синдром діабетичної стопи, діабетична мікроангіопатія, гемомікроциркуляторне русло, ішемія, гібридна артеріальна реконструкція.


2019 ◽  
Vol 71 (4) ◽  
pp. 703-710
Author(s):  
Slobodan Loncarevic ◽  
Denis Brajkovic ◽  
Milka Gardasevic ◽  
Olivera Loncarevic ◽  
Nebojsa Ladjevic ◽  
...  

Several studies have investigated the expression of tumor markers, including p53, HER-2, PCNA, EGFR, VEGFR CD-31 and Bcl-2 in patients with oral squamous carcinoma (OSC). This study aimed to determine the expression of proliferating cell nuclear antigen (PCNA), endothelial functions of platelet/endothelial cell adhesion molecule-1 (CD31) and human epidermal growth factor receptor-2 (HER-2) according to OSC stage. The prospective study included 62 patients diagnosed with OSC stages II and III. Surgical specimens were obtained from tumor and peritumoral tissues. We determined the pathohistological degree of tumor differentiation and the immunohistochemical expression of PCNA, CD-31 and HER-2 for each specimen. Immunohistochemical analysis of the expression of PCNA in tumor cells demonstrated poor staining of immunoreactive tumor cells in 23 patients (10 in stage II, 7 in stage IIIa and 6 in stage IIIb). Moderately expressed PCNA-immunoreactivity in the tumor cells in 17 patients (7 in stage II, 6 in stage IIIa and 4 in stage III), and extremely strong PCNA-immunoreactive staining in tumor cells of 10 patients with IIIb stage, was observed. These results suggest that PCNA expression combined with pathohistological findings could possess a prognostic value in determining the survival rates for patients with oral squamous cell carcinoma.


2019 ◽  
Vol 11 ◽  
pp. 175628721983777
Author(s):  
Ricardo G. Alvim ◽  
Christopher Hughes ◽  
Alexander Somma ◽  
Karan K. Nagar ◽  
Nathan C. Wong ◽  
...  

Objective: The objective of this study was to examine the impact of dehydrated human amnion/chorion membrane (dHACM) allografts on prostate and bladder cancer growth in the setting of residual disease and positive surgical margins. Materials and methods: A commercially available version of dHACM was used. Cytokines were identified and quantified, followed by comparative analysis of cell growth in two different human cell lines: prostate cancer (LNCaP) and bladder cancer (UM-UC-3), in vitro and in vivo. Tumor growth between the two groups, membrane versus no membrane implant, was compared and immunohistochemistry studies were conducted to quantify CD-31, Ki-67, and vimentin. A Student’s unpaired t-test was used to determine statistical significance. Results: The UM-UC-3 and LNCaP cells grew quicker in medium plus 10% serum and dHACM extract than in the other media ( p = 0.03). A total of 28 distinct cytokines were found in the extract, 11 of which had relatively high concentrations and are associated with prostate and bladder cancer tumor progression. In vivo LNCaP model, after 10 weeks, the median tumor volume in the membrane group was almost threefold larger than the partial resection alone ( p = 0.01). Two weeks after resection, in the UM-UC-3 model, the membrane group reached fourfold larger than the partial resection without membrane group ( p < 0.01). In both groups, the expression of CD-31 and Ki-67 markers were similar and showed no statistical significance ( p > 0.05). It was only in the LNCaP tumors that vimentin expression was significantly higher in the group without membrane compared with the membrane group ( p = 0.008). Conclusion: The use of dHACM after partial tumor resection is related to faster tumor relapse and growth in prostate and urothelial cancer in vivo models, showing a potential risk of rapid local recurrence in patients at high risk of positive margins.


2018 ◽  
Vol 620 ◽  
pp. A36 ◽  
Author(s):  
M. Schindewolf ◽  
P. Németh ◽  
U. Heber ◽  
T. Battich ◽  
M. M. Miller Bertolami ◽  
...  

Aims. Hot subdwarf stars represent a poorly understood late phase of stellar evolution. While binary evolution plays an important role for the formation of B-type subdwarfs (sdB), the origin of the helium dominated subclass of O-type subdwarfs (He-sdO) is still unknown. We search for chemical signatures of their genesis by means of quantitative spectral analyses of high-quality visual and ultraviolet spectra. Methods. Four prototypical He-sdO stars, one belonging to the nitrogen-rich and three to the C-rich subclass, were selected for which archival far-ultraviolet spectra from the FUSE satellite as well as high-resolution visual and UVA spectra taken with the ESO-UVES/FEROS spectrographs are available. Using T LUSTY200/S YNSPEC49 to compute line blanketed-non-local thermodynamic equilibrium (NLTE) model atmospheres and synthetic spectra, atmospheric parameters and the abundances patterns have been derived. The final models included H, He, C, N, O, Ne, Mg, Al, Si, P, S, Fe, and Ni represented by the most detailed model atoms available. Because of the enrichment of either nitrogen or carbon, it turned out, that models including these elements at the appropriate high abundance provide sufficiently accurate approximations to the temperature stratification of full models. Results. No indications for binarity were found, neither radial velocity variations nor photometric evidence for the presence of a companion could be detected. All stars have helium-dominated atmospheres almost free of hydrogen and temperatures between 42 000 K and 47 000 K while their surface gravities lie between log g = 5.4 and 5.7. The abundance pattern of CD–31°4800 displays the signatures of CNO burning, while heavier elements are subsolar by about 0.4 dex, except for Ne and Si which are close to solar. The abundance patterns of the C-rich He-sdOs are more complex. A slightly subsolar metallicity is accompanied by N-enrichment and O-deficiency, less pronounced than in CD–31°4800. Neon is mildly to strongly enriched, up to a factor of ten with respect to the sun in LS IV +10° 9. The nickel-to-iron ratio is significantly super-solar. Using spectral energy distributions and Gaia parallaxes the masses of the stars were determined. They are found to scatter around the canonical mass for the core helium flash, although the uncertainties are large. Conclusions. The abundance pattern observed for CD–31°4800 is consistent with predictions of models for slow (cold) mergers of pairs of equal mass helium WDs except for the low oxygen abundance observed. Models for composite mergers were considered for the C-rich stars, but predict abundance pattern dissimilar to those determined. [CW83] 0904−02, though, may be a candidate for a composite He-WD merger, as it rotates and appears to be more massive than the other program stars. New evolutionary models for the hot flasher scenario predict abundance patterns similar to those determined for the C-rich stars. Hence, C-rich He-sdO may well result from late He flashes with deep-mixing episodes.


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