Genetic diversity and statistical parameters of 15 autosomal STR loci in the Pomeranian Subpopulation of Espirito Santo State, Brazil

2010 ◽  
Vol 38 (5) ◽  
pp. 3013-3016 ◽  
Author(s):  
Beatriz Candida Silva ◽  
Eldamária de Vargas Wolfgramm ◽  
Vitor Resende da Costa Aguiar ◽  
Frederico Scott Varela Malta ◽  
Amanda Mafia de Castro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Statistical Parameters ◽  
Espírito Santo ◽  
Str Loci ◽  
Autosomal Str ◽  
Espirito Santo

scholarly journals Polymorphic microsatellites of analysis in cultivars of taro

2012 ◽  
Vol 30 (1) ◽  
pp. 106-111 ◽  
Author(s):  
Raquel SC Nunes ◽  
Fernanda R Pinhati ◽  
Luciana P Golinelli ◽  
Tiyoko Nair H Rebouças ◽  
Vânia Margaret F Paschoalin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Local Varieties ◽  
Mato Grosso ◽  
Espírito Santo ◽  
Polymorphic Microsatellites ◽  
Espirito Santo ◽  
Microsatellite Alleles ◽  
Mato Grosso Do Sul ◽  
Simple Sequence

Taro (Colocasia esculenta) is a tuberous plant belonging to the Araceae family whose tuber is the 14th most consumed food crop in the world. Characterized as an unconventional vegetable, taro is grown in Brazil as a subsistence crop, but in recent years began to gain commercial importance, especially in the states of Espirito Santo, Minas Gerais and Rio de Janeiro. To avoid loss of genetic diversity of the local varieties traditionally grown in Brazil a core collection for taro germplasm has been developed by the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural do estado do Espirito Santo (Incaper). The aim of this study was to perform a molecular characterization of the seven regional core collections. Genetic diversity of the cultivars was investigated by using SSR (Simple Sequence Repeats) polymorphisms, in seven loci (Xuqtem55, Xuqtem73, Xuqtem84, Xuqtem88, Xuqtem91, Xuqtem97 and Xuqtem110). Genetic diversity of the cultivars, based on the seven microsatellite alleles, was evaluated by using the software GelCompar II, showed that the loci Xuqtem73, Xuqtem88 and Xuqtem110 were the most informative, featuring 7, 10 and 8 alleles, respectively, a percentage of cultivars with polymorphic alleles of 85, 57 and 100% and identical PIC of 0.91. Based on Xuqtem110 locus analysis, the seven cultivars were grouped in two clusters. Chinês Regional Incaper cultivar was originated from Chinês cultivar which originated the São Bento cultivar, corroborating previous results. Macaquinho and Chinês cultivars were shown to be the primitive ones originating the allelic collections found in the states of Mato Grosso do Sul and Espirito Santo.

Genetic diversity of 17 autosomal STR loci in Meizhou Hakka population

2020 ◽  
Author(s):  
Xiaolong Han ◽  
Anna Shen ◽  
Ting Yao ◽  
Weibin Wu ◽  
Xiaohan Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Str Loci ◽  
Autosomal Str

scholarly journals Low Genetic Diversity of the Endangered Franciscana (Pontoporia blainvillei) in Its Northernmost, Isolated Population (FMAIa, Espírito Santo, Brazil)

2020 ◽  
Vol 7 ◽  
Author(s):  
Vanessa K. M. de Oliveira ◽  
Drienne M. Faria ◽  
Haydée A. Cunha ◽  
Teresa E. C. dos Santos ◽  
Adriana C. Colosio ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Control Region ◽  
Microsatellite Loci ◽  
Distribution Analysis ◽  
Sample Number ◽  
Espírito Santo ◽  
Low Genetic Diversity ◽  
Southwestern Atlantic Ocean ◽  
Espirito Santo ◽  
Pontoporia Blainvillei

The franciscana, Pontoporia blainvillei, is the most endangered small cetacean in the Southwestern Atlantic Ocean, occurring from Itaúnas, Espírito Santo, Brazil to Chubut province, Argentina. This area is divided into four Franciscana Management Areas (FMA). The northern portion of this species distribution is not continuous and a previous genetic study using mitochondrial DNA (mtDNA) separated it into FMAIa (Espírito Santo state) and FMAIb (North of Rio de Janeiro state). In order to increase the information about this population we expanded the sample number and evaluated mitochondrial and nuclear DNA diversity. Samples of 68 franciscanas found stranded on beaches from 2005 to 2020 were analyzed. Analyses included 350 bp of the mtDNA control region (D-loop) and 12 microsatellite loci. We identified three control region haplotypes in FMAIa, two of them not previously observed in this population, one being a new haplotype. Haplotype and nucleotide diversities were 0.0408 and 0.00012 respectively, the lowest reported for all FMAs analyzed until now. The Neutrality tests were not significant and Mismatch Distribution analysis did not reject the hypothesis of population expansion. One of the microsatellite loci was monomorphic, and for the other loci, two to nine alleles were identified, with expected heterozygosities ranging from 0.306 to 0.801. No substructure was revealed and effective population size (Ne) was estimated in 117.9 individuals. Even with an increased sample size, the high mitochondrial genetic homogeneity suggested for the population in a previous study was confirmed. Among six loci previously analyzed in other franciscana populations, five showed the lowest observed heterozygosities for the Espírito Santo population. The novel microsatellite data also showed low genetic diversity and could not reject the hypothesis of a single, panmitic population along the coast of Espírito Santo. This species has been intensively impacted in the last years by incidental capture during fishing activities and habitat degradation, caused by pollution, coastal development and environmental disasters in FMAIa. Considering that this population is small, isolated, and with low levels of genetic diversity, we reinforce the necessity of different conservation actions, focusing mainly on the reduction of bycatch of this species in the region.

Genetic analysis of 15 autosomal and 12 Y-STR loci in the Espirito Santo State population, Brazil

2011 ◽  
Vol 5 (3) ◽  
pp. e41-e43 ◽  
Author(s):  
Eldamária de Vargas Wolfgramm ◽  
Beatriz Candida Silva ◽  
Vitor Resende da Costa Aguiar ◽  
Frederico Scott Varela Malta ◽  
Amanda Mafia de Castro ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Espírito Santo ◽  
Str Loci ◽  
State Population ◽  
Espirito Santo

scholarly journals Genetic diversity of 23 autosomal STR loci in a Tibetan population

2017 ◽  
Vol 6 ◽  
pp. e101-e103 ◽  
Author(s):  
Yi Ye ◽  
Yu Liang ◽  
Haibo Luo ◽  
Ying Wang ◽  
Di Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Tibetan Population ◽  
Str Loci ◽  
Autosomal Str

scholarly journals Characterization and genetic diversity of Coffea canephora accessions in a germplasm bank in Espírito Santo, Brazil

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Maria Amélia Gava Ferrão ◽  
Rodolfo Ferreira de Mendonça ◽  
Aymbiré Francisco Almeida Fonseca ◽  
Romário Gava Ferrão ◽  
João Felipe Brites Senra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Coffea Canephora ◽  
Germplasm Bank ◽  
Espírito Santo ◽  
Espirito Santo

scholarly journals Genetic diversity in passion fruit plants at different altitudes

2019 ◽  
pp. 1083-1093
Author(s):  
Khétrin Silva Maciel ◽  
Paula Aparecida Muniz de Lima ◽  
Fernando Zanotti Madalon ◽  
Márcia Flores da Silva Ferreira ◽  
Rodrigo Sobreira Alexandre ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Passion Fruit ◽  
Similar Species ◽  
Passiflora Edulis ◽  
Espírito Santo ◽  
Espirito Santo ◽  
Different Altitudes

Passion fruit belongs to the Passifloraceae family and to the genus Passiflora, which is economically relevant. This genus is originated from Brazil, which has the greatest genetic diversity. The altitudinal gradient may influence the distribution of genetic variation in and between plant populations and genetic diversity may change according to the altitude. The objective of this study was to evaluate the genetic diversity in passion fruit in different altitudes of Espírito Santo, using microsatellite markers (SSR) and inter simple sequence repeat (ISSR). Five matrixes plants of each species of Passiflora edulis Sims f. flavicarpa Degener, P. edulis Sims and P. alata Curtis were studied at three altitudes (low, medium and high) in the state of Espírito Santo. For each sample, 13 microsatellite markers developed for Passiflora edulis and 14 ISSR primers were amplified according to the greatest number of polymorphic fragments and amplification quality. Low values of alleles were found (1-3), and low heterozygosity was expected and slight PIC values were found, while for the ISSR analysis a large number of bands per primer were detected and high polymorphism. The P. alata Curtis species was the most divergent to P. edulis Sims f. flavicarpa Deg. and P. edulis Sims. The SSR and ISSR markers enabled us to indicate differences among the species, but they did not always show variation between and within similar species. The populations of low altitude are different from the others, independent of the species and the marker used. The habitat has a highly important influence on genetic diversity.

scholarly journals Genetic diversity of begomovirus infecting tomato and associated weeds in Southeastern Brazil

2002 ◽  
Vol 27 (4) ◽  
pp. 372-377 ◽  
Author(s):  
LUCIANA P. AMBROZEVICIUS ◽  
RENATA F. CALEGARIO ◽  
ELIZABETH P.B. FONTES ◽  
MURILO G. DE CARVALHO ◽  
F. MURILO ZERBINI
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Rio De Janeiro ◽  
Minas Gerais ◽  
Southeastern Brazil ◽  
Espírito Santo ◽  
Chlorotic Mottle Virus ◽  
Chlorotic Mottle ◽  
Espirito Santo ◽  
High Degree

The genetic diversity of begomovirus isolates from tomato (Lycopersicon esculentum) fields in the Southeastern region of Brazil was analyzed by direct sequencing of PCR fragments amplified by using universal oligonucleotides for the begomovirus DNA-A, and subsequent computer-aided phylogenetic analysis. Samples of tomato plants and associated weeds showing typical symptoms of virus infection were collected at seven locations in the states of Minas Gerais, Espírito Santo and Rio de Janeiro. A total of 137 out of 369 samples were infected with a begomovirus based on PCR analysis. Phylogenetic analysis indicated a high degree of genetic diversity among begomoviruses infecting tomatoes in the sampled area. One species (Tomato chlorotic mottle virus, TCMV) occurs predominantly in Minas Gerais, whereas in Rio de Janeiro and Espírito Santo a distinct species, not yet fully characterized, predominates. Phylogenetic analysis further indicates the presence of an additional four possible new species. This high degree of genetic diversity suggests a recent transfer of indigenous begomovirus from wild hosts into tomatoes. The close phylogenetic relationship verified between begomovirus infecting tomato and associated weeds favors this hypothesis.

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