Polymorphism in the exon 4 of β-lactoglobulin variant B precursor gene and its association with milk traits and protein structure in Chinese Holstein

2011 ◽  
Vol 39 (4) ◽  
pp. 3957-3964 ◽  
Author(s):  
Fan Yang ◽  
Lian Li ◽  
Huiling Liu ◽  
Yafei Cai ◽  
Genlin Wang
Keyword(s):  
Protein Structure ◽  
Milk Traits ◽  
Chinese Holstein ◽  
Exon 4 ◽  
Β Lactoglobulin

scholarly journals Effects of SLC27A3 and β-Lactoglobulin Gene Polymorphisms on The Milk Composition in Hamdani Sheep

2021 ◽  
Author(s):  
Mervan Bayraktar ◽  
Omer Shoshin
Keyword(s):  
Allele Frequency ◽  
Milk Composition ◽  
Milk Traits ◽  
Breeding Programs ◽  
Exon 2 ◽  
Pcr Rflp ◽  
Milk Component ◽  
Component Traits ◽  
Exon 4 ◽  
Β Lactoglobulin

Abstract Background: SLC27A3 and β-Lactoglobulin gene are candidate genes that effects milk traits.Results: This study aimed to determine the effect of polymorphisms of the SLC27A3 gene on Hamdani sheep's milk components. The PCR-RFLP method was used to detect the SLC27A3 polymorphisms at exon 2, exon 3 and exon 4. The allele frequency of SNP1, SNP2 and SNP3 were 0.59(G), 0.41(T); 0.57(G), 0.43(C) and 0.58(A) and 0.42(C) respectively. β-lactoglobulin allele frequency was 0.56(A) and 0.44(B). The statistical analysis showed an association between SLC27A3 and β-lactoglobulin polymorphisms and milk composition. Conclusions: The SLC27A3 and β-lactoglobulin polymorphisms can be used as a genetic marker to select milk component traits in the sheep breeding programs.

scholarly journals Effect of β-lactoglobulin gene polymorphism, lactation stage and breed on milk traits in Chios and Karagouniko sheep breeds

2017 ◽  
Vol 17 (2) ◽  
pp. 371-384 ◽  
Author(s):  
Kostas A. Triantaphyllopoulos ◽  
Panagiota Koutsouli ◽  
Athanassios Kandris ◽  
Dimitris Papachristou ◽  
Kalliopi E. Markopoulou ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Traits ◽  
Weinberg Equilibrium ◽  
Sheep Breeds ◽  
Lactation Stage ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium ◽  
Daily Milk Yield ◽  
Β Lactoglobulin ◽  
Daily Milk

Abstract The animal selection with favourable phenotypes of the past has been, currently, replaced by the genotype selection on quantitative traits, assisted by the expanding molecular techniques in the context of livestock improvement. In this study, the c.112T>C polymorphism in exon II of β-lactoglobulin (β-LG) gene was investigated in Karagouniko and Chios sheep breeds by using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), and possible associations with milk traits were examined. In total, 125 blood DNA samples were isolated for PCR-RFLP analysis and the respective 217 milk samples′ composition profile was obtained. The goodness of fit test to Hardy-Weinberg equilibrium (HWE) for β-LG genotypes was estimated and associations found between β-LG genotypes and raw milk composition. Two alleles and three genotypes were observed (AA, AB and BB) in both breeds, and Chios breed significantly deviated (P≤0.05) from Hardy-Weinberg equilibrium (HWE). Conclusively, linear mixed model analysis on samples, from both breeds collectively, showed significant effects of β-LG genotype on lactose percentage and somatic cell count (SCC), lactation stage on daily milk yield and protein, while the breed effect was significant only on daily milk yield.

scholarly journals Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein

2006 ◽  
Vol 20 (2) ◽  
pp. 166-171 ◽  
Author(s):  
R. F. Zhang ◽  
H. Chen ◽  
C. Z. Lei ◽  
X. T. Fang ◽  
Y. D. Zhang ◽  
...  
Keyword(s):  
Milk Traits ◽  
Chinese Holstein ◽  
Pcr Rflp

scholarly journals Genetic association of variations in the kappa-casein and β-lactoglobulin genes with milk traits in girolando cattle

2019 ◽  
Vol 20 ◽  
Author(s):  
Severino Benone Paes Barbosa ◽  
Ítala Iara Medeiros de Araújo ◽  
Marta Fonseca Martins ◽  
Elizabete Cristina da Silva ◽  
Laís Aberrachid Jacopini ◽  
...  
Keyword(s):  
Milk Yield ◽  
Dairy Farm ◽  
Transmission Capacity ◽  
Farm Animals ◽  
Substitution Effects ◽  
Milk Traits ◽  
Pcr Rflp ◽  
Dairy Animals ◽  
Hardy Weinberg Equilibrium ◽  
Β Lactoglobulin

SUMMARY In dairy farm animals, one the most important goal of the selection is the improvement of milk yield and composition. Several studies have demonstrated that the candidate genes of the kappa-casein (CSN3) and β - lactoglobulin (β-LG) are associated with milk yield, milk quality and health traits in dairy animals. Therefore the aim of this study was to detect polymorphisms in CSN3 and β-LG genes and its association with milk yield in up to 305 days (305MY) and predicted transmission capacity (PTA) for 305MY in Girolando cattle. Totally, 138 bulls and 729 cows (n=867) were sampled. The genotypes of both genes were obtained by the PCR-RFLP method using HinfI and HaeIII enzymes for CSN3 and β-LG genes, respectively. Statistical results revealed two alleles A and B for both genes. The genotypes and alleles more frequents for CSN3 and β-LG genes were respectively: AA (0.7324) and A (0.8558), and AB (0.4827) and A (0.5017). The x2 test revealed that the two loci were at Hardy-Weinberg equilibrium (p<0.001). The allele substitution effects for the variants were not significant on 305MY and PTA for 305MY (p>0.05). The allele variants of β-LG and CSN3 might be more investigated before include them into future breeding schemes designed for Girolando dairy cattle with objective of improving milk traits as milk yield in up to 305 days (305MY) and predicted transmission capacity (PTA) for 305MY..

scholarly journals ASSOCIATION OF GENETIC VARIANTS OF β-LACTOGLOBULIN GENE WITH MILK TRAITS OF JEZEROPIVSKA SHEEP BREED

2020 ◽  
Vol 66 (3) ◽  
Author(s):  
Milena DJOKIC ◽  
Bozidarka MARKOVIC ◽  
Vesna GANTNER ◽  
Milan MARKOVIC
Keyword(s):  
Genetic Variants ◽  
Sheep Breed ◽  
Milk Traits ◽  
Β Lactoglobulin

scholarly journals β-Lactoglobulin Influences Human Immunity and Promotes Cell Proliferation

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Chun San Tai ◽  
Yi Yun Chen ◽  
Wen Liang Chen
Keyword(s):  
Cell Proliferation ◽  
Protein Structure ◽  
Immune Responses ◽  
Cell Model ◽  
Myeloma Cell ◽  
Promote Cell Proliferation ◽  
Chemically Modified ◽  
Human Immunity ◽  
Human Immune ◽  
Β Lactoglobulin

β-Lactoglobulin (LG) is suspected to enhance or modulate human immune responses. Moreover, LG is also hypothesized to increase human cell proliferation. However, these potential functions of LG have not been directly or thoroughly addressed. In this study, we demonstrated that LG is a potent stimulator of cell proliferation using a hybridoma cell (a splenocyte fused with a myeloma cell) model. LG’s ability to promote cell proliferation was lost when the protein is denatured. To further investigate the influence of LG’s conformation on cell proliferation, we chemically modified LG by either carboxymethylation (CM) or acetylation and observed significantly reduced cell proliferation when the protein structure was altered. Furthermore, we proved that LG enhances cell proliferation via receptor-mediated membrane IgM receptor. These data indicated that nondenatured LG is the major component in milk that modulates cell proliferation. Collectively, our study showed that LG plays a key role in enhancing immune responses by promoting cell proliferation through IgM receptor.

scholarly journals Polymorphism of β-Lactoglobulin Gene and their Effects on Milk Traits in Friesian X Bunaji Cattle

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Gabche A. E. Epse Laisin. ◽  
Adedibu I. I. ◽  
Kabir M. ◽  
Iyiola-Tunji A. O.
Keyword(s):  
Milk Traits ◽  
Β Lactoglobulin
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