Validation of reference genes for quantitative real-time PCR in valproic acid rat models of autism

2016 ◽  
Vol 43 (8) ◽  
pp. 837-847 ◽  
Author(s):  
Jinlong Zhou ◽  
Xiaozheng Zhang ◽  
Junrong Ren ◽  
Ping Wang ◽  
Junfeng Zhang ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Rat Models

scholarly journals rpoB and efp are stable candidate reference genes for quantitative real-time PCR analysis in Saccharopolyspora spinosa

2021 ◽  
Vol 35 (1) ◽  
pp. 619-632
Author(s):  
Xiaomeng Liu ◽  
Yunpeng Zhang ◽  
Kexue Huang ◽  
Tie Yin ◽  
Qi Li ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr ◽  
Saccharopolyspora Spinosa

Determination of the panel of reference genes for quantitative real-time PCR in fetal and adult rat intestines

2021 ◽  
Author(s):  
Xiaoqian Lu ◽  
Yi Liu ◽  
Dingmei Zhang ◽  
Kexin Liu ◽  
Qian Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Adult Rat

A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis

2009 ◽  
Vol 129 (1-2) ◽  
pp. 115-118 ◽  
Author(s):  
Yvette M. Schlotter ◽  
Eveline Z. Veenhof ◽  
Bas Brinkhof ◽  
Victor P.M.G. Rutten ◽  
Bart Spee ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Skin Biopsies ◽  
Healthy Dogs ◽  
Selection Of

scholarly journals Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

2018 ◽  
Vol 19 (8) ◽  
pp. 2258 ◽  
Author(s):  
Yuning Hu ◽  
Hongtuo Fu ◽  
Hui Qiao ◽  
Shengming Sun ◽  
Wenyi Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Reference Gene ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Translation Initiation Factor ◽  
Suitable Reference Gene ◽  
Quantitative Real Time Pcr ◽  
Macrobrachium Nipponense ◽  
The Stability ◽  
Suitable Reference

Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used. Recent studies have investigated suitable reference genes for some crustaceans under various conditions, but studies in Macrobrachium nipponense are currently lacking. In this study, we selected the following seven genes from among 35 commonly used housekeeping genes as candidate qPCR reference genes for temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S ribosomal RNA), EF-1α (elongation factor-1α), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (α-tubulin), β-act (β-actin), and RPL18 (Ribosomal protein L18). The stability of each reference gene was evaluated by GeNorm, NormFinder, BestKeeper, and comparative ∆C t methods, and was comprehensively ranked using RefFinder. RPL18 was shown to be the most suitable reference gene for adult M. nipponense tissues, while EIF was the most stable in different ovarian and embryo stages and in white spot syndrome virus infection, and β-act was the most stable reference gene under hypoxia stress. The reliability of the rankings was confirmed by RNA interference experiments. To the best of our knowledge, this represents the first systematic analysis of reference genes for qPCR experiments in M. nipponense, and the results will provide invaluable information for future research in closely related crustaceans.

scholarly journals Immune-Related Gene Expression Profile in Laboratory Common Marmosets Assessed by an Accurate Quantitative Real-Time PCR Using Selected Reference Genes

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56296 ◽  
Author(s):  
Yoshiki Fujii ◽  
Kazutaka Kitaura ◽  
Takaji Matsutani ◽  
Kenji Shirai ◽  
Satsuki Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Related Gene ◽  
Quantitative Real Time Pcr ◽  
Common Marmosets ◽  
Immune Related Gene
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