In vitro characterization of subventricular zone isolated neural stem cells, from adult monkey and rat brain

2021 ◽  
Author(s):  
Razieh Jaberi ◽  
Sara Mirsadeghi ◽  
Sahar Kiani
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
Subventricular Zone ◽  
In Vitro Characterization

In Vivo and in Vitro Characterization of the Angiogenic Effect of CTX0E03 Human Neural Stem Cells

2013 ◽  
Vol 22 (9) ◽  
pp. 1541-1552 ◽  
Author(s):  
Caroline Hicks ◽  
Lara Stevanato ◽  
Robert P. Stroemer ◽  
Ellen Tang ◽  
Sheila Richardson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Neural Stem Cells ◽  
In Vitro Characterization ◽  
Angiogenic Effect

scholarly journals Mesenchymal and neural stem cells labeled with HEDP-coated SPIO nanoparticles: In vitro characterization and migration potential in rat brain

2009 ◽  
Vol 1255 ◽  
pp. 18-31 ◽  
Author(s):  
Gaëtan J.-R. Delcroix ◽  
Matthieu Jacquart ◽  
Laurent Lemaire ◽  
Laurence Sindji ◽  
Florence Franconi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Rat Brain ◽  
In Vitro Characterization ◽  
Migration Potential ◽  
And Migration

Isolation and In Vitro Characterization of Epidermal Stem Cells

2017 ◽  
pp. 67-83 ◽  
Author(s):  
Kasper S. Moestrup ◽  
Marianne S. Andersen ◽  
Kim B. Jensen
Keyword(s):  
Stem Cells ◽  
Epidermal Stem Cells ◽  
In Vitro Characterization

scholarly journals Differentiation Induction as a Response to Irradiation in Neural Stem Cells In Vitro

Cancers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 913 ◽  
Author(s):  
Jana Konířová ◽  
Lukáš Cupal ◽  
Šárka Jarošová ◽  
Anna Michaelidesová ◽  
Jana Vachelová ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Brain Cancer ◽  
Subventricular Zone ◽  
Transcriptional Activity ◽  
Radiation Induced ◽  
Induction Of Differentiation ◽  
Stem Cell Populations ◽  
Neurocognitive Decline

Radiotherapy plays a significant role in brain cancer treatment; however, the use of this therapy is often accompanied by neurocognitive decline that is, at least partially, a consequence of radiation-induced damage to neural stem cell populations. Our findings describe features that define the response of neural stem cells (NSCs) to ionizing radiation. We investigated the effects of irradiation on neural stem cells isolated from the ventricular-subventricular zone of mouse brain and cultivated in vitro. Our findings describe the increased transcriptional activity of p53 targets and proliferative arrest after irradiation. Moreover, we show that most cells do not undergo apoptosis after irradiation but rather cease proliferation and start a differentiation program. Induction of differentiation and the demonstrated potential of irradiated cells to differentiate into neurons may represent a mechanism whereby damaged NSCs eliminate potentially hazardous cells and circumvent the debilitating consequences of cumulative DNA damage.

193 ROBUST PROPAGATION OF SELF-RENEWING PORCINE NEURAL STEM CELLS ISOLATED FROM TRANSGENIC PIGS WITH A GFAP-CreERT2 SYSTEM CAPABLE OF CONTROLLING THE EXPRESSION OF EGFP GENES

2017 ◽  
Vol 29 (1) ◽  
pp. 205
Author(s):  
E. Kim ◽  
H. Kim ◽  
S.-H. Hyun
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Subventricular Zone ◽  
Central Nervous System Diseases ◽  
Transgenic Pigs ◽  
Ample Evidence ◽  
Viable Cells ◽  
Promising Source ◽  
Dna Recombinase

Ample evidence has demonstrated the important roles of pigs because their anatomical, immunologic, and physiological characteristics are fairly similar to humans. In particular, their gyrencephalic brain are more comparable to humans than rodents with similar grey and white matter composition and size. In this study, we isolated and propagated the neural stem cells (GFAP-CreERT2-NSCs) from the transgenic piglet with expression of CreERT2, a fusion protein of the DNA recombinase Cre and mutated ligand-binding domain of the human oestrogen receptor, under the control of the GFAP promoter. The primary culture from tissue of porcine CreERT2 brain led to floating spherical masses of cells that revealed similar morphology and size distribution to neurospheres reported by previous studies. Quantitative analysis indicated a yield of 2.50 ± 0.44 primary spheres per 1,000 viable cells from the neocortex, versus 12.92 ± 1.67 primary spheres per 1,000 viable cells from the periventricular region (PVR) including subventricular zone. Secondary spheres (6.67 ± 1.10 spheres from neocortex versus 23.08 ± 1.96 spheres from PVR cells) were formed from primary spheres at 10 days after passage. Tertiary spheres (8.42 ± 0.99 spheres from neocortex versus 23.08 ± 1.91 spheres from PVR cells) could also be obtained after a second passage, indicating that they were proliferating in vitro. The CreERT2-NSCs showed normal 36+XY karyotype and representative NSC markers, such as NESTIN, SOX2, and VIMENTIN. After differentiation, we were able to obtain populations of astrocytes and neurons expressing GFAP and TUJ1, respectively. In summary, we verified and propagated the isolated GFAP promoter-driven CreERT2-NSCs, which would be considered a promising source of cells for treatment of central nervous system diseases.

Isolation and in Vitro Characterization of Bovine Amniotic Fluid Derived Stem Cells at Different Trimesters of Pregnancy

2014 ◽  
Vol 10 (5) ◽  
pp. 712-724 ◽  
Author(s):  
B. Rossi ◽  
B. Merlo ◽  
S. Colleoni ◽  
E. Iacono ◽  
P. L. Tazzari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Fluid ◽  
In Vitro Characterization

Identification and Characterization of Secondary Neural Tube-Derived Embryonic Neural Stem Cells In Vitro

2015 ◽  
Vol 24 (10) ◽  
pp. 1171-1181 ◽  
Author(s):  
Mohammed R. Shaker ◽  
Joo Yeon Kim ◽  
Hyun Kim ◽  
Woong Sun
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Neural Tube ◽  
Identification And Characterization
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