Human serum albumin as protecting agent of silver nanoparticles: role of the protein conformation and amine groups in the nanoparticle stabilization

2013 ◽  
Vol 15 (1) ◽  
Author(s):  
Emilio I. Alarcon ◽  
Carlos J. Bueno-Alejo ◽  
Christopher W. Noel ◽  
Kevin G. Stamplecoskie ◽  
Natalia L. Pacioni ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Conformation ◽  
Nanoparticle Stabilization ◽  
Amine Groups

Interaction of human serum albumin with silver nanoparticles functionalized with polyvinylthiol

2015 ◽  
Vol 204 ◽  
pp. 248-254 ◽  
Author(s):  
Mohd. Sajid Ali ◽  
Hamad A. Al-Lohedan ◽  
Ayman M. Atta ◽  
Abdelrahman O. Ezzat ◽  
Sami A.A. Al-Hussain
Keyword(s):  
Silver Nanoparticles ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum

scholarly journals Role of cationic carbosilane dendrons and metallic core of functionalized gold nanoparticles in their interaction with human serum albumin

2018 ◽  
Vol 118 ◽  
pp. 1773-1780 ◽  
Author(s):  
Dzmitry Shcharbin ◽  
Elzbieta Pedziwiatr-Werbicka ◽  
Tatyana Serchenya ◽  
Sylwia Cyboran-Mikolajczyk ◽  
Lena Prakhira ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Metallic Core ◽  
Functionalized Gold Nanoparticles

scholarly journals Secretion of Human Serum Albumin by Kluyveromyces lactis Overexpressing KlPDI1 and KlERO1

2005 ◽  
Vol 71 (8) ◽  
pp. 4359-4363 ◽  
Author(s):  
Tiziana Lodi ◽  
Barbara Neglia ◽  
Claudia Donnini
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Conformation ◽  
Protein Production ◽  
Heterologous Protein ◽  
Kluyveromyces Lactis ◽  
Heterologous Proteins ◽  
Disulfide Bridges ◽  
Genes Encoding

ABSTRACT The control of protein conformation during translocation through the endoplasmic reticulum is often a bottleneck for heterologous protein production. The core pathway of the oxidative folding machinery includes two conserved proteins: Pdi1p and Ero1p. We increased the dosage of the genes encoding these proteins in the yeast Kluyveromyces lactis and evaluated the secretion of heterologous proteins. KlERO1, an orthologue of Saccharomyces cerevisiae ERO1, was cloned by functional complementation of the ts phenotype of an Scero1 mutant. The expression of KlERO1 was induced by treatment of the cells with dithiothreitol and by overexpression of human serum albumin (HSA), a disulfide bond-rich protein. Duplication of either PDI1 or ERO1 led to a similar increase in HSA yield. Duplication of both genes accelerated the secretion of HSA and improved cell growth rate and yield. Increasing the dosage of KlERO1 did not affect the production of human interleukin 1β, a protein that has no disulfide bridges. The results confirm that the ERO1 genes of S. cerevisiae and K. lactis are functionally similar even though portions of their coding sequence are quite different and the phenotypes of mutants overexpressing the genes differ. The marked effects of KlERO1 copy number on the expression of heterologous proteins with a high number of disulfide bridges suggests that control of KlERO1 and KlPDI1 is important for the production of high levels of heterologous proteins of this type.

Role of surface charge on the interaction between carbon nanodots and human serum albumin

2018 ◽  
Vol 204 ◽  
pp. 484-494 ◽  
Author(s):  
Meng-Ying Li ◽  
Chang-Qing Xiao ◽  
Zi-Qiang Xu ◽  
Miao-Miao Yin ◽  
Qi-Qi Yang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Charge ◽  
Carbon Nanodots

Binding of cationic Cm-E2O-Cm gemini surfactants with human serum albumin and the role of β-cyclodextrin

2020 ◽  
Vol 312 ◽  
pp. 113365 ◽  
Author(s):  
Mohd. Akram ◽  
Farah Ansari ◽  
Faizan Abul Qais ◽  
Kabir-ud-Din
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Gemini Surfactants

The role of human serum albumin and neurotoxin associated proteins in the formulation of BoNT/A products

Toxicon ◽  
2019 ◽  
Vol 168 ◽  
pp. 158-163 ◽  
Author(s):  
Anna Kutschenko ◽  
Hans Bigalke ◽  
Florian Wegner ◽  
Kai Wohlfarth
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Associated Proteins
Sign in / Sign up

Export Citation Format

Share Document