scholarly journals Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment

2012 ◽  
Vol 110 (3) ◽  
pp. 305-313 ◽  
Author(s):  
Rintaro Hashizume ◽  
Ivan Smirnov ◽  
Sharon Liu ◽  
Joanna J. Phillips ◽  
Jeanette Hyer ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Diffuse Intrinsic Pontine Glioma ◽  
Glioma Cell Line ◽  
Pontine Glioma

scholarly journals Characterization of cells recovered from the xenotransplanted NG97 human-derived glioma cell line subcultured in a long-term in vitro

BMC Cancer ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Camila ML Machado ◽  
Rafael Y Ikemori ◽  
Tatiana Q Zorzeto ◽  
Ana CMA Nogueira ◽  
Suse DS Barbosa ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line

scholarly journals OLIG2 maintenance is not essential for diffuse intrinsic pontine glioma cell line growth but regulates tumor phenotypes

2021 ◽  
Author(s):  
Yunfei Liao ◽  
Zaili Luo ◽  
Yaqi Deng ◽  
Feng Zhang ◽  
Rohit Rao ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Line ◽  
Therapeutic Potential ◽  
Malignant Gliomas ◽  
Diffuse Intrinsic Pontine Glioma ◽  
Glioma Cell Line ◽  
Egfr Signaling ◽  
Pontine Glioma ◽  
Genomic Landscape ◽  
Genome Bisulfite Sequencing

Abstract Background Diffuse intrinsic pontine glioma (DIPG) is a pediatric lethal high-grade brainstem glioma with no effective therapies. OLIG2 was reported to be critical for the growth of a DIPG cell line CCHMC-DIPG-1. Surprisingly, we found that the CCHMC-DIPG-1 cells express little OLIG2 and exhibit a mesenchymal phenotype, which raised a question regarding the role of OLIG2 in the growth of DIPG cells. Methods We evaluated the function of OLIG2 in different DIPG cell lines through molecular and genetic approaches and performed transcriptomic and genomic landscape profiling including whole-genome bisulfite-sequencing, RNA-seq, ATAC-seq, and ChIP-seq. shRNA-mediated knockdown and CRISPR-Cas9-mediated knockout approaches were utilized to assess OLIG2 functions in DIPG cell growth. Results We found that DIPG cells are phenotypically heterogeneous and exhibit the characteristics of distinct malignant gliomas including proneural, classical, and mesenchymal subtypes. OLIG2 knockdown did not impact the growth of CCHMC-DIPG-1 cells, wherein OLIG2 is epigenetically silenced. Moreover, OLIG2 deletion did not substantially impair OLIG2-expressing proneural-like DIPG growth but led to an upregulation of HIPPO-YAP1 and EGFR signaling and a tumor phenotype shift. Targeting HIPPO-YAP1 and EGFR signaling in OLIG2-deficient DIPG cells inhibited tumor cell growth. Conclusions Our data indicate that OLIG2 is dispensable for DIPG growth but regulates the phenotypic switch of DIPG tumor cells. OLIG2 downregulation leads to deregulation of adaptive YAP1 and EGFR signaling. Targeting YAP1 and EGFR pathways inhibits the growth of OLIG2-deficient DIPG cells, pointing to a therapeutic potential by targeting adaptive signaling to treat DIPG tumors with nominal OLIG2 expression.

Synthesis and photophysical characterization of quasi push–pull dicyanodibenzodioxins and their anti-tumor activity against glioma cell line C6

2015 ◽  
Vol 25 (4) ◽  
pp. 753-757 ◽  
Author(s):  
Subhadeep Banerjee ◽  
Anjan Chattopadhyay ◽  
Arnab Banerjee ◽  
Meera Haridas ◽  
Praveen Saini ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line ◽  
Anti Tumor Activity

Ultrastructural characterization of the new NG97ht human-derived glioma cell line using two different electron microscopy technical procedures

2009 ◽  
Vol 72 (4) ◽  
pp. 310-316 ◽  
Author(s):  
Camila Maria Longo Machado ◽  
Tatiane Queiroz Zorzeto ◽  
Juares E. Romero Bianco ◽  
Renata Giardini Rosa ◽  
Selma Candelaria Genari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line ◽  
Ultrastructural Characterization

scholarly journals Characterization of new gangliosides of the lactotetraose series in murine xenografts of a human glioma cell line

FEBS Letters ◽  
1986 ◽  
Vol 201 (1) ◽  
pp. 109-113 ◽  
Author(s):  
J.-E. Månsson ◽  
P. Fredman ◽  
D.D. Bigner ◽  
K. Molin ◽  
B. Rosengren ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line ◽  
Human Glioma ◽  
Human Glioma Cell Line ◽  
Human Glioma Cell

Characterization of hyaluronan synthase from a human glioma cell line

1998 ◽  
Vol 1380 (3) ◽  
pp. 377-388 ◽  
Author(s):  
Tomas Asplund ◽  
Jonas Brinck ◽  
Masanobu Suzuki ◽  
Michael J Briskin ◽  
Paraskevi Heldin
Keyword(s):  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line ◽  
Hyaluronan Synthase ◽  
Human Glioma ◽  
Human Glioma Cell Line ◽  
Human Glioma Cell

Characterization of an established human malignant glioma cell line: LN-18

1981 ◽  
Vol 53 (1) ◽  
pp. 21-28 ◽  
Author(s):  
A. C. Diserens ◽  
N. de Tribolet ◽  
A. Martin-Achard ◽  
A. C. Gaide ◽  
J. F. Schnegg ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Cell Line ◽  
Glioma Cell ◽  
Glioma Cell Line ◽  
Human Malignant Glioma

Characterization of a continuous human glioma cell line DBTRG-05MG: growth kinetics, karyotype, receptor expression, and tumor suppressor gene analyses

1992 ◽  
Vol 28 (9-10) ◽  
pp. 609-614 ◽  
Author(s):  
Carol A. Kruse ◽  
Dawn H. Mitchell ◽  
Bette K. Kleinschmidt-DeMasters ◽  
Wilbur A. Franklin ◽  
Helvise G. Morse ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Cell Line ◽  
Tumor Suppressor Gene ◽  
Growth Kinetics ◽  
Glioma Cell ◽  
Suppressor Gene ◽  
Glioma Cell Line ◽  
Receptor Expression ◽  
Human Glioma
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